Nanohybrids of Magnetically Intercalated Optical Metamaterials for Magnetic Resonance/Raman Imaging and <i>In Situ</i> Chemodynamic/Photothermal Therapy

Jibin, Kunnumpurathu; Victor, Marina; Saranya, G.; Santhakumar, Hema; Murali, Vishnupriya; Maiti, Kaustabh Kumar; Jayasree, Ramapurath S.

doi:10.1021/acsabm.1c00510

Cited by 28 publications

(26 citation statements)

References 58 publications

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“…SERS spectroscopy is well known for its selectivity and sensitivity as compared to other imaging modalities. It is capable of detecting even a single molecule or single cell with high multiplexing efficacies. , The main challenge encountered during this work was to reduce the nonspecific binding of the Au-rGO@anti-ErbB2 SERS nanotag to other blood cells, which otherwise outstrip CTCs by several orders of magnitude. Initially, in order to increase the sensitivity of detection, control experiments were carried out to nullify the external factors which could lead to false negative results.…”

Section: Resultsmentioning

confidence: 99%

“…The synthesis of the probe was initiated with the synthesis of Au-rGO (gold-reduced graphene oxide nanosystem) which is described elsewhere. , The as-prepared Au-rGO was incubated with PEG molecules (methoxy-PEG, SH-PEG-COOH) to provide high surface coverage and stability to the nanohybrid . After 3 h, free PEG molecules were removed by continuous rounds of washing and centrifugation (8000 rpm, 20 min), and the pellet was dispersed in PBS.…”

Section: Materials and Methodsmentioning

confidence: 99%

“…Owing to these unique detection attributes, SERS-based substrates have been increasingly utilized as bioprobes for the detection of biological components for the last decade. Further, one of the vital advantages of using SERS rather than a conventional fluorescence technique is that SERS gives a specific and sharp fingerprint-like spectral pattern, which is free from other interferences, thereby facilitating a highly sensitive detection response within the complex biological environment. − So, fabrication of a highly efficient CTC isolation platform tethered with a sensitive detection technique such as SERS readouts delivers a promising podium for efficient detection of CTCs.…”

Section: Introductionmentioning

confidence: 99%

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Graphene–Gold Nanohybrid-Based Surface-Enhanced Raman Scattering Platform on a Portable Easy-to-Use Centrifugal Prototype for Liquid Biopsy Detection of Circulating Breast Cancer Cells

Jibin

Babu

Jayasree

2021

ACS Sustainable Chem. Eng.

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Although, cancer is considered as a localized disease in its premature stages, in certain types of cells, it results in metastasis, which accounts for over 90% of the total cancer deaths in the world. During the metastatic stage, cancer cells migrate from the primary tumor site to secondary sites through the circulating bloodstream, resulting in metastasis at unexpected body parts. This situation makes it critical to identify and quantify the circulating tumor cells (CTCs) during the early stages of tumorigenesis. Despite the clinical importance and progress of liquid biopsy and CTC-based cancer diagnostics, it still remains extremely challenging to develop systems to detect CTCs in cancer patients as its presence is extremely low in number (1−10 CTCs per mL of blood plasma). In this article, we discuss about the design and fabrication of a robust and user-friendly custom-designed nanotag-enabled portable filter-based sensor system for the selective separation and isolation of circulating breast cancer cells from whole blood. The filter sensor platform basically consists of a centrifugal prototype to quickly transfer unprocessed blood samples up to 5 mL within 60 s, isolating the circulating breast cancer cells selectively over the anti-EpCAM antibody-immobilized polycarbonate (PC) filter. Moreover, it is also equipped with a sandwich complex of a surface-enhanced Raman scattering (SERS) nanotag comprising a gold− graphene hybrid integrated with anti-ErbB2 antibody (Au-rGO@anti-ErbB2) for the SERS-assisted quantification of CTCs. As compared to the immunoaffinity-based CTC isolation platforms, the centrifugal force-based SERS nanotag enabled filter sensor system proved to be a clog free, highly sensitive, and selective platform toward the rapid isolation of viable breast cancer cells up to 5 tumor cells/mL from whole blood without any sample pretreatment. The high sensitivity, ease of use, and quick availability of results recommends the current filter sensor platform as a universal liquid biopsy technique for the quantification of CTCs.

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Section: Resultsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Graphene–Gold Nanohybrid-Based Surface-Enhanced Raman Scattering Platform on a Portable Easy-to-Use Centrifugal Prototype for Liquid Biopsy Detection of Circulating Breast Cancer Cells

Jibin

Babu

Jayasree

2021

ACS Sustainable Chem. Eng.

Self Cite

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“…Hydrogen peroxide (H 2 O 2 ), hypochlorite acid (HOCl), ultraoxygen anion (O 2− ·), and hydroxyl free radical (·OH), collectively known as reactive oxygen species (ROS), [ 92 ] play an essential role in cancer processes, such as oncogene expression, [ 93 ] apoptosis, [ 94 ] angiogenesis, [ 95 ] and cell signal transduction. [ 96 ] As a characteristic feature of the tumor microenvironment, high levels of ROS promote the invasion of cancer cells by activating epidermal growth factor receptors (EGFRs).…”

Section: Tumor Metabolite‐responsive Sers Probesmentioning

confidence: 99%

Emergence of Responsive Surface‐Enhanced Raman Scattering Probes for Imaging Tumor‐Associated Metabolites

Yin

Jin

Duan

et al. 2022

Adv Healthcare Materials

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As a core hallmark of cancer, metabolic reprogramming alters the metabolic networks of cancer cells to meet their insatiable appetite for energy and nutrient. Tumor-associated metabolites, the products of metabolic reprogramming, are valuable in evaluating tumor occurrence and progress timely and accurately because their concentration variations usually happen earlier than the aberrances demonstrated in tissue structure and function. As an optical spectroscopic technique, surface-enhanced Raman scattering (SERS) offers advantages in imaging tumor-associated metabolites, including ultrahigh sensitivity, high specificity, multiplexing capacity, and uncompromised signal intensity. This review first highlights recent advances in the development of stimuli-responsive SERS probes. Then the mechanisms leading to the responsive SERS signal triggered by tumor metabolites are summarized. Furthermore, biomedical applications of these responsive SERS probes, such as the image-guided tumor surgery and liquid biopsy examination for tumor molecular typing, are summarized. Finally, the challenges and prospects of the responsive SERS probes for clinical translation are also discussed.

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“…In addition, owing to the presence of Mn 2+ , Fe 3 O 4 @MnO 2 can be used as a contrast agent for enhanced T 1 -weighted magnetic resonance imaging (MRI). 33 After the consumption of MnO 2 , the exposed core Fe 3 O 4 can react with endogenous H 2 O 2 to generate ·OH, which can further kill tumor cells. Moreover, MnO 2 can decompose H 2 O 2 to O 2 to alleviate hypoxia.…”

Section: Introductionmentioning

confidence: 99%

Effective Combination of Isoniazid and Core-Shell Magnetic Nanoradiotherapy Against Gastrointestinal Tumor Cell Types

Chen¹,

Zhu²,

Guo³

et al. 2022

IJN

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Introduction Radiotherapy is a conventional treatment for gastrointestinal tumors. However, its therapeutic effect might not be satisfactory because of factors such as radio-resistance of tumor cells and dose reduction applied to avoid damage to normal tissues. We developed a novel combination therapy involving the use of isoniazid (INH) and core-shell magnetic nanospheres (NPs) to enhance the efficacy of radiotherapy. Methods Magnetic core-shell NPs were synthesized. The shell manganese dioxide (MnO 2 ) reacted with intracellular glutathione to produce Mn 2+ , which decomposed hydrogen peroxide (H 2 O 2 ) to hydroxyl radicals (·OH) in the presence of INH to produce sufficient amount of reactive oxygen species. In addition to this chemodynamic therapy, MnO 2 catalyzed H 2 O 2 to O 2 , which alleviated hypoxia in tumors and thus enhanced the effect of radiotherapy. In addition, iron oxide (Fe 3 O 4 ) and reduced Mn 2+ were potential candidates for T 1 –T 2 dual-mode magnetic resonance imaging (MRI) with remarkable magnetic targeting ability. Results NPs exhibited efficient tumor targeting performance under the magnetic field and improved T 1 /T 2 dual-mode MRI, which elevated oxygen levels without toxicity to the mice to achieve remarkable therapeutic outcomes, reaching a tumor inhibition rate of 93.2%. Moreover, chemodynamic therapy mediated by INH and NPs enhanced the therapeutic effect of radiotherapy both in vivo and in vitro. Conclusion The results demonstrated that the combination of INH and NPs could be a novel strategy for radiosensitization with clinical potential.

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Nanohybrids of Magnetically Intercalated Optical Metamaterials for Magnetic Resonance/Raman Imaging and In Situ Chemodynamic/Photothermal Therapy

Cited by 28 publications

References 58 publications

Graphene–Gold Nanohybrid-Based Surface-Enhanced Raman Scattering Platform on a Portable Easy-to-Use Centrifugal Prototype for Liquid Biopsy Detection of Circulating Breast Cancer Cells

Graphene–Gold Nanohybrid-Based Surface-Enhanced Raman Scattering Platform on a Portable Easy-to-Use Centrifugal Prototype for Liquid Biopsy Detection of Circulating Breast Cancer Cells

Emergence of Responsive Surface‐Enhanced Raman Scattering Probes for Imaging Tumor‐Associated Metabolites

Effective Combination of Isoniazid and Core-Shell Magnetic Nanoradiotherapy Against Gastrointestinal Tumor Cell Types

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