Nanoindentation and whole-bone bending estimates of material properties in bones from the senescence accelerated mouse SAMP6

Silva, Matthew J.; Brodt, Michael D.; Fan, Zaifeng; Rho, Jae-Young

doi:10.1016/j.jbiomech.2004.02.018

Cited by 98 publications

(90 citation statements)

References 36 publications

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“…Both the strain gauge data and FEA results indicated that the tibia of the SAMP6 mouse was approximately 20 -25% stiffer than the SAMR1 tibia. The increased stiffness of the SAMP6 tibia is consistent with the increased bending rigidity and moment of inertia (Silva et al, 2002) and the increased elastic modulus of cortical bone (Silva et al, 2004a) that we have documented previously for the SAMP6 mouse compared to SAMR1. Therefore, the two different mouse strains had unique forceendocortical strain relations and higher levels of force are required to produce the same target values of strain in the SAMP6 tibia compared to SAMR1.…”

Section: Discussionsupporting

confidence: 89%

“…Another strength of our methods was that we assigned cortical bone modulus a priori based on a previous nanoindentation study in which we reported a small but significant difference of 10% in modulus between SAMP6 and SAMR1 bones (Silva et al, 2004a). Therefore, differences in material stiffness were accounted for in this study and contributed to the differences in stiffness between the two mouse strains.…”

Section: Discussionmentioning

confidence: 99%

“…Cortical bone modulus was assigned a priori based on a previous study (Silva et al, 2004a). In that study, we measured the nanoindentation modulus of dry tibial samples and reported average values of 30 GPa for SAMP6 and 28 GPa for SAMR1.…”

Section: Finite-element Analysis Methodsmentioning

confidence: 99%

“…However, for FEA, we needed to assign modulus values for wet bone at the continuum (macroscopic) level. Based on relative comparisons of nanoindentation and continuum modulus values taken from the literature (Reilly and Burstein, 1975;Rho et al, , 2002Turner et al, 1999), we estimated that the continuum modulus of wet bone is approximately two-thirds the nanoindentation modulus of dried bone (Silva et al, 2004a). Thus, for the finiteelement model, we scaled the measured nanoindentation values and assigned values of 20 and 18.5 GPa for SAMP6 and SAMR1 cortical bone, respectively.…”

Section: Finite-element Analysis Methodsmentioning

confidence: 99%

“…Because SAMP6 and SAMR1 tibiae have different bone sizes (Silva et al, 2002) and different values of cortical bone modulus (Silva et al, 2004a), and also because there is no way to measure endocortical strains directly, we employed finite-element analysis (FEA). Our objective was to develop a method to estimate the peak endocortical strains generated during three-point bending of the tibia in SAMP6 and control SAMR1 mice in order to estimate the forces needed to produce target endocortical strains of 1,000 and 2,000 microstrain.…”

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confidence: 99%

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Finite element analysis of the mouse tibia: Estimating endocortical strain during three‐point bending in SAMP6 osteoporotic mice

2005

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To support future studies of tibial bending in a murine model of senile osteoporosis (SAMP6), we sought to determine the relationship between applied external bending force and peak endocortical strain in the tibiae of SAMP6 and control SAMR1 mice. The lower hindlimbs of mice were loaded by three-point bending in the lateral-medial plane with a support length of 10 mm. Force-periosteal strain relations were first determined using standard strain gauge methods. Finite-element analysis (FEA) models of the tibia-fibula were generated based on microcomputed tomography images. After choosing appropriate boundary conditions, FEA predictions of periosteal strains were within 15% of measured values. FEA revealed a narrow (3-4 mm) region of the central tibia with well-developed bending strains (tension medially, compression laterally); outside this region, we observed high shear strains. Both the strain gauge data and the finite-element simulations indicated that the tibia of the SAMP6 mouse was 20 -25% stiffer than the SAMR1 tibia, consistent with a larger moment of inertia and higher cortical bone modulus. Thus, higher levels of force are required to produce the same target values of strain in the SAMP6 tibia. The ratio of periosteal to endocortical strain in the region of interest was similar for the two mouse strains (1.5-1.6). Based on these ratios, we scaled the strain gauge data to estimate the force-endocortical strain relations for the two mouse strains. In conclusion, FEA, with supporting strain gauge measurements, has provided unique insight regarding the strain environment throughout the tibia during three-point bending in mice.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Finite-element Analysis Methodsmentioning

confidence: 99%

Section: Finite-element Analysis Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Finite element analysis of the mouse tibia: Estimating endocortical strain during three‐point bending in SAMP6 osteoporotic mice

2005

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Osteoporosis and Osteopetrosis

Boskey¹

2007

Handbook of Biomineralization

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Osteopontin deficiency and aging on nanomechanics of mouse bone

Kavukcuoglu

Denhardt

Güzelsu

et al. 2007

J Biomedical Materials Res

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Osteoporosis is a bone disease characterized by low bone mass and deterioration of the tissue leading to increased fragility. Osteopontin (OPN), a noncollageneous bone matrix protein, has been shown to play an important role in osteoporosis, bone resorption, and mineralization. However, OPN's role in bone mechanical properties on the submicron scale has not been studied in any detail. In this study, nanoindentation techniques were utilized to investigate how OPN and aging affect bone mechanical properties. Hardness and elastic modulus were calculated and compared between the OPN-deficient mice (OPN(-/-)) and their age and sex-matched wild-type (OPN(+/+)) controls. The results show that the mechanical properties of the young OPN(-/-) bones (age < 12 weeks) are significantly lower than that of the youngest OPN(+/+) bones. This finding was confirmed by additional microindentation testing. Biochemical analysis using micro-Raman spectroscopy indicated more mineral content in young OPN(+/+) bones. Older (age > 12 weeks) bones did not show any significant differences in mechanical properties with genotype. In addition, OPN(+/+) bones show a decrease in mechanical properties between young and older age groups. By contrast, OPN(-/-) bones showed no significant change in mechanical properties with aging.

show abstract

Nanoindentation and whole-bone bending estimates of material properties in bones from the senescence accelerated mouse SAMP6

Cited by 98 publications

References 36 publications

Finite element analysis of the mouse tibia: Estimating endocortical strain during three‐point bending in SAMP6 osteoporotic mice

Finite element analysis of the mouse tibia: Estimating endocortical strain during three‐point bending in SAMP6 osteoporotic mice

Osteoporosis and Osteopetrosis

Osteopontin deficiency and aging on nanomechanics of mouse bone

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