NanoJ: a high-performance open-source super-resolution microscopy toolbox

Laine, Romain F.; Tosheva, Kalina L.; Gustafsson, Nils; Gray, Robert D.; Almada, Pedro; Albrecht, David; Risa, Gabriel Tarrason; Hurtig, Fredrik; Lindås, Ann-Christin; Baum, Buzz; Mercer, Jason; Leterrier, Christophe; Pereira, Pedro M.; Culley, Siân; Henriques, Ricardo

doi:10.1101/432674

Cited by 31 publications

(41 citation statements)

References 54 publications

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“…Islets were allowed to equilibrate for another 2 min, followed by multiple imaging series of 320-s cycles. Image series were concatenated, drift was corrected by the Nano J-Core ImageJ plugin (75), and intensity profiles and kymographs were generated for quantification.…”

Section: Static Secretionmentioning

confidence: 99%

Primary cilia control glucose homeostasis via islet paracrine interactions

Hughes

Cho

Conway

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Pancreatic islets regulate glucose homeostasis through coordinated actions of hormone-secreting cells. What underlies the function of the islet as a unit is the close approximation and communication among heterogeneous cell populations, but the structural mediators of islet cellular cross talk remain incompletely characterized. We generated mice specifically lacking β-cell primary cilia, a cellular organelle that has been implicated in regulating insulin secretion, and found that the β-cell cilia are required for glucose sensing, calcium influx, insulin secretion, and cross regulation of α- and δ-cells. Protein expression profiling in islets confirms perturbation in these cellular processes and reveals additional targets of cilia-dependent signaling. At the organism level, the deletion of β-cell cilia disrupts circulating hormone levels, impairs glucose homeostasis and fuel usage, and leads to the development of diabetes. Together, these findings demonstrate that primary cilia not only orchestrate β-cell–intrinsic activity but also mediate cross talk both within the islet and from islets to other metabolic tissues, thus providing a unique role of cilia in nutrient metabolism and insight into the pathophysiology of diabetes.

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Section: Static Secretionmentioning

confidence: 99%

Primary cilia control glucose homeostasis via islet paracrine interactions

Hughes

Cho

Conway

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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“…CdvB is part of the ESCRT-III-mediated cell division ring in archaea, and was previously suggested to be a core part of the cell division machinery (15)(16)(17)31). Given this, why would an increase in the level of this key division protein be associated with the inability to divide?…”

Section: Proteasomal Targets During Cell Divisionmentioning

confidence: 99%

Proteasome-mediated protein degradation resets the cell division cycle and triggers ESCRT-III-mediated cytokinesis in an archaeon

Risa

Hurtig

Bray

et al. 2019

Preprint

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The archaeon Sulfolobus acidocaldarius is a relative of eukaryotes known to progress orderly through its cell division cycle despite lacking obvious CDK/cyclin homologues. Here, in exploring the mechanisms underpinning archaeal cell division cycle control, we show that the proteasome of S. acidocaldarius, like its eukaryotic counterpart, regulates the transition from the end of one cell division cycle to the beginning of the next. Further, we identify the archaeal ESCRT-III homologue CdvB as a key target of the proteasome, and show that state-dependent degradation of CdvB triggers archaeal cell division by allowing constriction of a CdvB1:CdvB2 ESCRT-III division ring. These findings suggest an ancient role for proteasome-mediated degradation in resetting the cell division cycle in both archaea and eukaryotes. ESCRT-III | Cell cycle | Evolution | Proteasome

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“…Graph in Supp. Notes S1.2) was compensated using image post-processing inside the localization software ThunderSTORM [52] or the Nano-J suite [53] in Fiji [49]. A second MQTT-enabled smartphone acting as a remote control enables the coupling efficiency and focus position to be adjusted without touching the imaging device.…”

Section: Sr Reconstruction Of Compressed and Raw Measurements And Flumentioning

confidence: 99%

Nanoscopy on the Chea(i)p

Diederich

Helle

Then

et al. 2020

Preprint

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Super-resolution microscopy allows for stunning images with a resolution well beyond the optical diffraction limit, but the imaging techniques are demanding in terms of instrumentation and software. Using scientific-grade cameras, solid-state lasers and top-shelf microscopy objective lenses drives the price and complexity of the system, limiting its use to well-funded institutions. However, by harnessing recent developments in CMOS image sensor technology and low-cost illumination strategies, super-resolution microscopy can be made available to the mass-markets for a fraction of the price. Here, we present a 3D printed, self-contained super-resolution microscope with a price tag below 1000 $ including the objective and a cellphone. The system relies on a cellphone to both acquire and process images as well as control the hardware, and a photonic-chip enabled illumination. The system exhibits 100nm optical resolution using single-molecule localization microscopy and can provide live super-resolution imaging using light intensity fluctuation methods. Furthermore, due to its compactness, we demonstrate its potential use inside bench-top incubators and high biological safety level environments imaging SARS-CoV-2 viroids. By the development of low-cost instrumentation and by sharing the designs and manuals, the stage for democratizing super-resolution imaging is set.

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NanoJ: a high-performance open-source super-resolution microscopy toolbox

Cited by 31 publications

References 54 publications

Primary cilia control glucose homeostasis via islet paracrine interactions

Primary cilia control glucose homeostasis via islet paracrine interactions

Proteasome-mediated protein degradation resets the cell division cycle and triggers ESCRT-III-mediated cytokinesis in an archaeon

Nanoscopy on the Chea(i)p

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