Nanoleakage evaluation at adhesive-dentin interfaces by different observation methods

Abstract

The purpose of this study was to evaluate the capability and characteristics of different nanoleakage observation methods, including light microscope (LM), field-emission scanning electron microscope (FESEM), transmission electron microscope (TEM), and confocal laser scanning microscope (CLSM). Dentin specimens were bonded with either an etch-and-rinse adhesive (SBMP) or a self-etch adhesive (GB), and prepared for nanoleakge evaluation according to different observation methods. LM, FESEM and CLSM results demonstrated that the SBMP group showed more interfacial nanoleakage than the GB group (p<0.05); by contrast, no significant difference was found in TEM results (p>0.05), however, TEM illustrated concrete nanoleakage forms or patterns. The results suggested that different observation methods might exhibit distinct images and a certain degree of variations in nanoleakage statistical results. Researchers should carefully design and calculate the optimum assembly in combination with qualitative and quantitative approaches to obtain objective and accurate nanoleakage evaluation.

Effects of calcium-containing desensitizers on the bonding stability of an etch-and-rinse adhesive against long-term water storage and pH cycling

“…For example, the MTBS of all groups significantly decreased after pH cycling, and the nanoleakage expression obviously increased. This trend echoes the general consensus that high bonding strengths usually correspond to low nanoleakage expression 30,31) . The present study evaluated the effects of two calcium-containing desensitizing pastes on the bonding durability and stability of an E&R adhesive to dentine in vitro.…”

Effects of different etching modes on the nanoleakage of universal adhesives: A systematic review and meta‐analysis

“…FESEM and TEM were the most frequently used methods for observing nanoleakage . FESEM provides additional data and high‐contrast images compared with SEM .…”

Quercetin as a simple but versatile primer in dentin bonding

“…Therefore, a general evaluation of nanoleakage along the bonding interface is necessary. 30 Aer collagenase challenge ( Fig. 3E and F), we found that in some area along the hybrid layer, the morphology of the metallic silver grains in the control group was similar to the "water trees" described by Tay.…”

“…This result might provide support to Hashimoto and Yang, whose researches found no direct relationship between the values of resin-dentin bond strength and the percent of nanoleakage. 30,45 In contrast, Ekambaram et al found that, with the increase in nanoleakage, the resin-dentin bond strength decreased to some degree.…”

“…The failure modes were classied into four groups: (A) adhesive failure; (CD) cohesive failure in dentin; (CC) cohesive failure in composite; and (M) mixed failure. 30 …”