Nanoliter-scale, regenerable ion sensor: sensing with surface functionalized microstructured optical fiber

Heng, Sabrina; Nguyen, Mai-Chi; Kostecki, Roman; Monro, Tanya M.; Abell, Andrew D.

doi:10.1117/12.2016510

Cited by 5 publications

(5 citation statements)

References 34 publications

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“…Each fiber was precleaned and hydroxylated by immersion in nitric acid followed by piranha solution and then functionalized with alternating layers of poly(allylamine hydrochloride) (PAH), poly(acrylic acid) (PAA) and further PAH 26 for the exposed core fiber and APTES for the suspended core fiber. 27 The SP2maltose-liposomes were then coupled onto to the surfacebound free amines using standard coupling conditions 27 (HATU/DIPEA; Supporting Information) to give the functionalized exposed core and suspended core sensors. The ability of the exposed core fiber-based sensor to bind and detect Zn 2+ was studied using the optical set up depicted in Figure 5.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Microstructured Optical Fiber-based Biosensors: Reversible and Nanoliter-Scale Measurement of Zinc Ions

Heng

McDevitt

Kostecki

et al. 2016

ACS Appl. Mater. Interfaces

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The new agreement specifically addresses what authors can do with different versions of their manuscript -e.g. use in theses and collections, teaching and training, conference presentations, sharing with colleagues, and posting on websites and repositories. The terms under which these uses can occur are clearly identified to prevent misunderstandings that could jeopardize final publication of a manuscript (Section II, Permitted Uses by Authors). Easy Reference User Guide Posting Accepted and Published Works on Websites and Repositories:A digital file of the Accepted Work and/or the Published Work may be made publicly available on websites or repositories (e.g. the Author's personal website, preprint servers, university networks or primary employer's institutional websites, third party institutional or subject-based repositories, and conference websites that feature presentations by the Author(s) based on the Accepted and/or the Published Work) under the following conditions:• It is mandated by the Author(s)' funding agency, primary employer, or, in the case of Author(s) employed in academia, university administration.• If the mandated public availability of the Accepted Manuscript is sooner than 12 months after online publication of the Published Work, a waiver from the relevant institutional policy should be sought. If a waiver cannot be obtained, the Author(s) may sponsor the immediate availability of the final Published Work through participation in the ACS AuthorChoice program-for information about this program see http://pubs.acs.org/page/policy/authorchoice/index.html.• If the mandated public availability of the Accepted Manuscript is not sooner than 12 months after online publication of the Published Work, the Accepted Manuscript may be posted to the mandated website or repository. The following notice should be included at the time of posting, or the posting amended as appropriate: "This document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request authordirected link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html]." • The posting must be for non-commercial purposes and not violate the ACS' "Ethical Guidelines to Publication of Chemical Research" (see http://pubs.acs.org/ethics).• Regardless of any mandated public availability date of a digital file of the final Published Work, Author(s) may make this file available only via the ACS AuthorChoice Program. For more information, see http://pubs.acs.org/page/policy/authorchoice/index.html. sensor provides a significant advantage in that it allows the use of nL sampling when compared to ICP-MS (mL) and . This work paves the way to a generic approach for developing surface-based ion sensors using a range of sensor molecules, which can be attached to a surface without the need for its chemical modifica...

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Section: ■ Results and Discussionmentioning

confidence: 99%

Microstructured Optical Fiber-based Biosensors: Reversible and Nanoliter-Scale Measurement of Zinc Ions

Heng

McDevitt

Kostecki

et al. 2016

ACS Appl. Mater. Interfaces

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“…Details on the synthesis of SHL are reported in the synthesis section of the Supplemental Information. In brief, the Li 1 sensor was prepared from 1-aza-15-crown-5 and 1-(2-hydroxyethyl)-2,3,3-trimethyl-3H-indol-1ium to give SHL with an overall yield of 20% using a modification to existing methodology (Heng et al, 2013;Stubing et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Development of a Photoswitchable Lithium-Sensitive Probe to Analyze Nonselective Cation Channel Activity in Migrating Cancer Cells

et al. 2019

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This is the first work to use a newly designed Li 1-selective photoswitchable probe Sabrina Heng Lithium (SHL) in living colon cancer cells to noninvasively monitor cation channel activity in real time by the appearance of lithium hot spots detected by confocal microscopy. Punctate Li 1 hot spots are clustered in the lamellipodial leading edges of HT29 human colon cancer cells and are colocalized with aquaporin-1 (AQP1) channels. AQP1 is a dual water and cyclic-nucleotidegated cation channel located in lamellipodia and is essential for rapid cell migration in a subset of aggressive cancers. Both the Li 1 hot spots and cell migration are blocked in HT29 cells by the AQP1 ion channel antagonist AqB011. In contrast, Li 1 hot spots are not evident in a poorly migrating colon cancer cell line, SW620, which lacks comparable membrane expression of AQP1. Knockdown of AQP1 by RNA interference in HT29 cells significantly impairs Li 1 hot spot activity. The SHL probe loaded in living cells shows signature chemical properties of ionic selectivity and reversibility. Dynamic properties of the Li 1 hot spots, turning on and off, are confirmed by time-lapse imaging. SHL is a powerful tool for evaluating cation channel function in living cells in real time, with particular promise for studies of motile cells or interlinked networks not easily analyzed by electrophysiological methods. The ability to reset SHL by photoswitching allows monitoring of dynamic signals over time. Future applications of the Li 1 probe could include high-throughput optical screening for discovering new classes of channels, or finding new pharmacological modulators for nonselective cation channels.

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“…Benzylic bromide ( 2 ) was prepared by reaction of the N -substituted benzylaldehyde with paraformaldehyde in the presence of HBr. Reaction of these halides with bis(2-pyridylmethyl) amine gave 3 , respectively, which was reacted with 4 , 39 with final purification by normal-phase chromatography, giving the desired sensor 5 , as detailed in the Supporting Information section. 1 H, 13 C NMR spectra for sensor 5 can be found in the Supporting Information section.…”

Section: Resultsmentioning

confidence: 99%

“…Compound 4 (Scheme 1) was prepared as previously described. 39 1 H and 13 C NMR spectra were recorded on a Varian 500 MHz and Varian Inova 600 MHz instruments in the indicated solvents. Chemical shifts are reported in ppm (δ).…”

Section: ■ Conclusionmentioning

confidence: 99%

Rationally Designed Probe for Reversible Sensing of Zinc and Application in Cells

Heng¹,

Reineck

Vidanapathirana³

et al. 2017

ACS Omega

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Biologically compatible fluorescent ion sensors, particularly those that are reversible, represent a key tool for answering a range of fundamental biological questions. We report a rationally designed probe with a 6′-fluoro spiropyran scaffold (5) for the reversible sensing of zinc (Zn2+) in cells. The 6′-fluoro substituent overcomes several limitations normally associated with spiropyran-based sensors to provide an improved signal-to-background ratio and faster photoswitching times in aqueous solution. In vitro studies were performed with 5 and the 6′-nitro analogues (6) in HEK 293 and endothelial cells. The new spiropyran (5) can detect exogenous Zn2+ inside both cell types and without affecting the proliferation of endothelial cells. Studies were also performed on dying HEK 293 cells, with results demonstrating the ability of the key compound to detect endogenous Zn2+ efflux from cells undergoing apoptosis. Biocompatibility and photoswitching of 5 were demonstrated within endothelial cells but not with 6, suggesting the future applicability of sensor 5 to study intracellular Zn2+ efflux in these systems.

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Nanoliter-scale, regenerable ion sensor: sensing with surface functionalized microstructured optical fiber

Cited by 5 publications

References 34 publications

Microstructured Optical Fiber-based Biosensors: Reversible and Nanoliter-Scale Measurement of Zinc Ions

Microstructured Optical Fiber-based Biosensors: Reversible and Nanoliter-Scale Measurement of Zinc Ions

Development of a Photoswitchable Lithium-Sensitive Probe to Analyze Nonselective Cation Channel Activity in Migrating Cancer Cells

Rationally Designed Probe for Reversible Sensing of Zinc and Application in Cells

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