Nanoliter-scale, regenerable ion sensor: sensing with a surface functionalized microstructured optical fibre

Heng, Sabrina; Nguyen, Mai-Chi; Kostecki, Roman; Monro, Tanya M.; Abell, Andrew D.

doi:10.1039/c3ra40321a

Cited by 55 publications

(44 citation statements)

References 40 publications

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“…One possible alternative to solution-based measurements is to chemically attach the fluorophore to a surface to allow localised measurement without releasing the fluorophore. [7][8][9] Thus, by immobilising a H 2 O 2 sensitive fluorophore peroxyfluor-1 (PF1) to glass surfaces, detection of H 2 O 2 can be performed without potential detrimental effects on the embryo.…”

Section: Introductionmentioning

confidence: 99%

Localised hydrogen peroxide sensing for reproductive health

et al. 2015

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Authors, or their employers in the case of works made for hire, retain the following rights: 1. All proprietary rights other than copyright, including patent rights. 2. The right to make and distribute copies of the Paper for internal purposes. 3. The right to use the material for lecture or classroom purposes. 4. The right to prepare derivative publications based on the Paper, including books or book chapters, journal papers, and magazine articles, provided that publication of a derivative work occurs subsequent to the official date of publication by SPIE.5. The right to post an author-prepared version or an official version (preferred version) of the published paper on an internal or external server controlled exclusively by the author/employer, provided that (a) such posting is noncommercial in nature and the paper is made available to users without charge; (b) a copyright notice and full citation appear with the paper, and (c) a link to SPIE's official online version of the abstract is provided using the DOI (Document Object Identifier) link. Citation format:Author(s), "Paper Title," Publication Title, Editors, Volume (Issue) Number, Article (or Page) Number, (Year). Copyright notice format:Copyright XXXX (year) Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. ABSTRACTThe production of reactive oxygen species (ROS) is known to affect the developmental competence of embryos. Hydrogen peroxide (H 2 O 2 ) an important reactive oxygen species, is also known to causes DNA damage and defective sperm function. Current techniques require incubating a developing embryo with an organic fluorophore which is potentially hazardous for the embryo. What we need is a localised ROS sensor which does not require fluorophores in solution and hence will allow continuous monitoring of H 2 O 2 production without adversely affect the development of the embryo. Here we report studies on such a fibre-based sensor for the detection of H 2 O 2 that uses a surface-bound aryl boronate fluorophore carboxyperoxyfluor-1(CPF1). Optical fibres present a unique platform due to desirable characteristics as dip sensors in biological solutions. Attempts to functionalise the fibre tips using polyelectrolyte layers and (3-aminopropyl)triethoxysilane (APTES) coatings resulted in a limited signal and poor fluorescent response to H 2 O 2 due to a low tip surface density of the fluorophore. To increase the surface density, CPF1 was integrated into a polymer matrix formed on the fibre tip by a UV-catalysed polymerisation process of acrylamide onto a methacrylate silane layer. The polyacrylamide containing CPF1 gave a much higher surface density than previous surface attachment methods and the sensor was found to effectively detect H 2 O 2 . Using this method, biologically relevant concentrations of H 2 O 2 ...

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Section: Introductionmentioning

confidence: 99%

Localised hydrogen peroxide sensing for reproductive health

et al. 2015

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“…By the suitable selection of glass or polymer material and geometry the dispersion, nonlinearity, birefringence, polarization, evanescent field and mode area of the propagating light can be optimized to specific applications. This has led to innovations in supercontinuum generation [4], fiber lasers [5], terahertz wave guiding [6], fibers with high numerical apertures [7,8], sensors [9][10][11][12], and makes MOFs an excellent candidate for new high-capacity transmission multicore and endlessly single mode telecommunications fibers [13].…”

Section: Introductionmentioning

confidence: 99%

Predicting the drawing conditions for Microstructured Optical Fiber fabrication

Kostecki¹,

Ebendorff-Heidepriem²,

Warren‐Smith³

et al. 2013

Opt. Mater. Express

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The efficient and accurate fabrication of Microstructured optical fibers (MOFs) requires a practical understanding of the 'draw process' beyond what is achievable by trial and error, which requires the ability to predict the experimental drawing parameters needed to produce the desired final geometry. Our results show that the Fitt et al. fluid-mechanics model for describing the draw process of a single axisymmetric capillary fiber provides practical insights when applied to more complex multi-hole symmetric and asymmetric MOF geometries. By establishing a method to relate the multi-hole MOF geometry to a capillary and understanding how material temperature varies with the draw tower temperature profile, it was found that analytical equations given by the Fitt model could be used to predict the parameters necessary for the chosen structure. We show how this model provides a practical framework that contributes to the efficient and accurate fabrication of the desired MOF geometries by predicting suitable fiber draw conditions.

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“…For such applications it is necessary to immobilize sensor molecules on the glass surface of the MOF exposed core, which can then be used directly within the medium as a sensor without requiring prior pre-mixing of analyte with sensor molecules. Functionalization methods traditionally used include silanes [7,8] or polyelectrolytes [9,10], which provide a functional group on the surface to which the sensor molecules can be covalently attached. These processes require several steps that typically take many hours to perform, due to the incubation times needed to achieve consistent maximized binding efficiency [8,10].…”

Section: Introductionmentioning

confidence: 99%