Nanomaterial-based electrochemical biosensors for cytochrome c using cytochrome c reductase

Manickam, Pandiaraj; Thangamuthu, Madasamy; Gollavilli, Paradesi Naidu; Muthukutty, Balamurugan; Kotamraju, Srigiridhar; Rao, Venkat K.; Bhargava, Kalpana; Karunakaran, C.

doi:10.1016/j.bioelechem.2012.09.004

Cited by 62 publications

(26 citation statements)

References 37 publications

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“…Figure 11. Furthermore, the CcR-CNT based cyt c biosensor was applied to quantify the cytosolic cyt c released from the mitochondria of apoptotic human lung carcinoma A549 cells and the results were validated with the standard western blot analysis (Pandiaraj et al, 2013b).…”

Section: Cytochrome C Reductase Based Biosensormentioning

confidence: 99%

“…The detection limit of the sensor is 2 nM with a dynamic range spanning to 1 µM. (Zhao et al, 2008) sBLM/GNP/PC/LA/GCE LSV 0.1 µM -3.2 µM 50 nM (Liu and Wei, 2008) CcO/NiONPs/MWCNT/PANI/Au CV 5 pM -0.5 µM 5 pM (Batra et al, 2013) CcO/DDAB/Au SWV 0.2 µM -800 µM 0.2 µM (Ashe et al, 2007) CcR/SAM/GNP/PPy/Pt CV 5 µM -600 µM 2 µM (Pandiaraj et al, 2013b) CcR/CNT/PPy/Pt CV 1 µM -1000 µM 0.5 µM (Pandiaraj et al, 2013b) CcR/CNT/PPy/SPE CV 50 nM -500 µM 50 nM (Pandiaraj et al, 2014a) anti-cyt c/SAM/GNP/PPy/SPE CV 2 nM -150 µM 2 nM (Pandiaraj et al, 2014b) anti-cyt c/CNT/PPy/SPE CV 10 nM -50 µM 10 nM (Pandiaraj et al, 2014b) PEG/Aptamer/Epoxy-Graphite EIS 50 pM -50 nM 63.2 pM (Ocaña et al, 2014) Aptamer/EDC-NHS/MUA/Gold SPR 80 nM -80 pM ≥50 pM (Loo et al, 2014) DNA-Cellulose/CPE DPV 0.1 mM -1µM 0.5 µM (Lee et al, 2004) Single-strand DNA (ssDNA), Supported bilayer lipid membrane (sBLM), Soybean Phosphatidylcholine, (PC), Lauric acid (LA), Glassy carbon electrode (GCE), Nickel oxide nanoparticles (NiONPs), Polyaniline (PANI), didodecyldimethylammonium bromide (DDAB), Polyethylene glycol (PEG), 11-mercaptoundecanoic acid (MUA), Carbon paste electrode (CPE).…”

Section: Aptamer Based Cyt C Biosensorsmentioning

confidence: 99%

“…Schematic illustration of electron transport through various active sites of mitochondrial complex III.Our group reported a novel approach for the detection of oxidized form of cyt c (Fe 3+ ) for the first time using CcR immobilized on nanoparticles decorated electrodes(Pandiaraj et al, 2014a(Pandiaraj et al, , 2013a. Two different kinds of nanomaterial decorated biosensor platforms were used for the construction of biosensors:(a) carbon nanotubes (CNT) incorporated polypyrrole (PPy) matrix on Pt electrode and (b) self-assembled monolayer (SAM) functionalized gold nanoparticles (GNP) in PPy-Pt.…”

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confidence: 99%

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Recent advances in cytochrome c biosensing technologies

Manickam

Kaushik

Karunakaran³

et al. 2017

Biosensors and Bioelectronics

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This review is an attempt, for the first time, to describe advancements in sensing technology for cytochrome c (cyt c) detection, at point-of-care (POC) application. Cyt c, a heme containing metalloprotein is located in the intermembrane space of mitochondria and released into bloodstream during pathological conditions. The release of cyt c from mitochondria is a key initiative step in the activation of cell death pathways. Circulating cyt c levels represents a novel in-vivo marker of mitochondrial injury after resuscitation from heart failure and chemotherapy. Thus, cyt c detection is not only serving as an apoptosis biomarker, but also is of great importance to understand certain diseases at cellular level. Various existing techniques such as enzyme-linked immunosorbent assays (ELISA), Western blot, high performance liquid chromatography (HPLC), spectrophotometry and flow cytometry have been used to estimate cyt c. However, the implementation of these techniques at POC application is limited due to longer analysis time, expensive instruments and expertise needed for operation. To overcome these challenges, significant efforts are being made to develop electrochemical biosensing technologies for fast, accurate, selective, and sensitive detection of cyt c. Presented review describes the cutting edge technologies available in the laboratories to detect cyt c. The recent advancements in designing and development of electrochemical cyt c biosensors for the quantification of cyt c are also discussed. This review also highlights the POC cyt c biosensors developed recently, that would prove of interest to biologist and therapist to get real time informatics needed to evaluate death process, diseases progression, therapeutics and processes related with mitochondrial injury.

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Section: Cytochrome C Reductase Based Biosensormentioning

confidence: 99%

Section: Aptamer Based Cyt C Biosensorsmentioning

confidence: 99%

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confidence: 99%

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Recent advances in cytochrome c biosensing technologies

Manickam

Kaushik

Karunakaran³

et al. 2017

Biosensors and Bioelectronics

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“…Nanotechnolgy is defined as the creation of functional systems through control of matter at the 1–100 nm scale. Nanomaterials exhibit interesting physical, chemical, and electronic properties such as size and shape dependent surface plasmon resonance, high surface energy and surface to volume ratio and tunable surface properties [ 18 , 19 ]. For example, the physical properties of nanomaterials can be explored for cell imaging, drug delivery systems and to design optical assays and bioassays.…”

Section: Functions Of Nanomaterialsmentioning

confidence: 99%

Current Trends in Nanomaterial-Based Amperometric Biosensors

Hayat

Catanante

Marty

2014

Sensors

107

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The last decade has witnessed an intensive research effort in the field of electrochemical sensors, with a particular focus on the design of amperometric biosensors for diverse analytical applications. In this context, nanomaterial integration in the construction of amperometric biosensors may constitute one of the most exciting approaches. The attractive properties of nanomaterials have paved the way for the design of a wide variety of biosensors based on various electrochemical detection methods to enhance the analytical characteristics. However, most of these nanostructured materials are not explored in the design of amperometric biosensors. This review aims to provide insight into the diverse properties of nanomaterials that can be possibly explored in the construction of amperometric biosensors.

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“…Despite its good selectivity and low detection limits, it requires extensive, time-consuming sample preparation and derivatization steps prior to sample analysis. [18][19][20][21] Hence, there is growing interest in the development of direct and indirect electrochemical methods for the determination of HcySH. chemiluminescence, 14 fluorescence, 15 and UV-Vis detection protocols, 16,17 which are also employed for clinical HcySH assays, involve expensive reagents and instrumentation systems.…”

Section: Introductionmentioning

confidence: 99%

A miniaturized electrochemical assay for homocysteine using screen-printed electrodes with cytochrome c anchored gold nanoparticles

Thangamuthu

Santschi

Martin

2015

Analyst

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Determination of homocysteine (HcySH) is highly beneficial in human physiology and pathophysiology for diagnosis and prognosis of cardiovascular diseases (CVD). Unfortunately, the practicability of the existing methodologies for the determination of HcySH is limited in terms of sample requirements, preparation time and instrumentation cost. To overcome these limitations, we have developed a new miniaturized electrochemical assay for HcySH in which cytochrome c (cyt c) immobilized on gold nanoparticle (GNP) modified screen printed carbon electrode (SPE) is employed as a biosensing element. The electrochemical characterization of the biosensor (cyt c-GNP-SPE) shows quasi-reversible redox peaks at the potentials 0 and -0.2 V, confirming the cyt c binding. The methodology of quantification is based on the electrochemical oxidation of HcySH by the Fe(3+)/Fe(2+) crevice of cyt c, observed at a potential of +0.56 V. Using the amperometric technique, the detection limit of HcySH is found to be 0.3 ± 0.025 μM in the linear range between 0.4 μM and 700 μM, with a sensitivity of 3.8 ± 0.12 nA μM(-1) cm(-2). The practical application of the present assay is validated through the measurement of HcySH in blood plasma samples and the selectivity is ensured by eliminating the impact of the common interfering biological substrates using a Nafion membrane. This biosensor shows striking analytical properties of good repeatability, reproducibility (2.85% SD) and high stability (83% of its initial current response after 4 weeks). This work paves the way for cheap, efficient and reliable point-of-care biosensors for screening one of the major causes of deaths both in the developed and developing countries.

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Nanomaterial-based electrochemical biosensors for cytochrome c using cytochrome c reductase

Cited by 62 publications

References 37 publications

Recent advances in cytochrome c biosensing technologies

Recent advances in cytochrome c biosensing technologies

Current Trends in Nanomaterial-Based Amperometric Biosensors

A miniaturized electrochemical assay for homocysteine using screen-printed electrodes with cytochrome c anchored gold nanoparticles

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