Nanomaterials-Based Optical Techniques for the Detection of Acetylcholinesterase and Pesticides

Xia, Ning; Wang, Qinglong; Liu, Lin

doi:10.3390/s150100499

Cited by 61 publications

(30 citation statements)

References 73 publications

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“…Biosensors for inhibition of AChE have been thus developed for the detection of pesticides. In line with the rapid development of nanotechnology, nanomaterials have attracted great attention and have been intensively studied in biological analysis due to their unique chemical, physical and size properties (Xia et al, 2015).…”

Section: Inhibition Of Acetylcholinesterase (Ache) By Pesticidesmentioning

confidence: 99%

Effect of some Plants and Pesticides on Acetylcholinesterase

Khorshid¹,

Hassan²,

El-Gawad³

et al. 2015

American J. of Food Technology

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The principal role of acetylcholinesterase (AChE)is the termination of nerve impulse transmission at the cholinergic synapses by rapid hydrolysis of acetylcholine (ACh). Inhibition of AChE serves as a strategy for the treatment of Alzheimer's Disease (AD). Several acetylcholinesterase inhibitors (AChEIs) are used for the symptomatic treatment of AD A potential source of AChE inhibitors is certainly provided by the abundance of plants in nature. The aim of this article is study the effect of some plants and pesticides on acetylcholinesterase. Several plants considered as a source of acetylcholinesterase inhibitors and pesticides special organophosphorus.

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Section: Inhibition Of Acetylcholinesterase (Ache) By Pesticidesmentioning

confidence: 99%

Effect of some Plants and Pesticides on Acetylcholinesterase

Khorshid¹,

Hassan²,

El-Gawad³

et al. 2015

American J. of Food Technology

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“…Their degradation is mediated by plants and microorganisms through their metabolites [3]. In addition to the abovementioned conventional methods that are used for the detection and detoxification of pesticides, in recent years, nanotechnology based protocols have been developed to address these issues [4][5][6][7]. In general, iron, gold, silver and silica nanoparticles are used for such applications [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Silica nanoparticle based techniques for extraction, detection, and degradation of pesticides

Bapat

Labade

Chaudhari

et al. 2016

Advances in Colloid and Interface Science

100

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“…Low‐cost, simple, and sensitive methods for the determination of organophosphorus and carbamate pesticides are therefore crucial for both environmental monitoring and diagnosis . Many pesticide‐detection methods are currently available . Among these methods, electrochemical methods have been widely employed because of their simple operation and small instrument size, which make them particularly well suited for point‐of‐care testing and on‐site detection .…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Electrochemical Detection of Carbaryl Based on Enzyme Inhibition and Thiocholine Oxidation Mediated by a Ruthenium(III) Complex

Fang

Park

et al. 2016

Electroanalysis

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In the electrochemical detection method for pesticides that measures their inhibitory effects on acetylcholinesterases (AChEs), the direct electrooxidation of the enzyme product (thiocholine, SCh) is slow at conventional electrodes. To overcome this limitation, an electron mediator is required to lower the applied potential and facilitate the transfer of electrons between the enzyme product and electrode. In this study, [Ru(NH3)5py]3+ is introduced as an electron mediator in inhibition‐based pesticide detection. To obtain a better signal‐to‐background ratio, [Ru(NH3)5py]3+, which undergoes a fast outer‐sphere reaction, is combined with low‐electrocatalytic indium‐tin‐oxide (ITO) electrodes at which many interfering species undergo slow redox reactions. AChE is immobilized onto an avidin‐modified ITO electrode via the direct adsorption of avidin onto ITO followed by the biospecific binding of biotinylated AChE to the avidin. SCh is generated from acetylthiocholine by AChE. Subsequently, SCh converts [Ru(NH3)5py]3+ to [Ru(NH3)5py]2+, which is then oxidized at the ITO electrode. This procedure allows the sensitive detection of carbaryl at a low applied potential of 0.15 V vs Ag/AgCl. The calculated detection limit for carbaryl is approximately 0.3 pM. This simple and sensitive pesticide sensor is thus very promising and should be extendable to the onsite environmental monitoring of other pesticides.

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Nanomaterials-Based Optical Techniques for the Detection of Acetylcholinesterase and Pesticides

Cited by 61 publications

References 73 publications

Effect of some Plants and Pesticides on Acetylcholinesterase

Effect of some Plants and Pesticides on Acetylcholinesterase

Silica nanoparticle based techniques for extraction, detection, and degradation of pesticides

Rapid and Sensitive Electrochemical Detection of Carbaryl Based on Enzyme Inhibition and Thiocholine Oxidation Mediated by a Ruthenium(III) Complex

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