Nanomechanical properties of individual chondrocytes and their developing growth factor-stimulated pericellular matrix

Ng, Laurel; Hung, Han-Hwa; Sprunt, Alexander D.; Chubinskaya, Susan; Ortiz, Christine; Grodzinsky, Alan J.

doi:10.1016/j.jbiomech.2006.04.004

Cited by 81 publications

(88 citation statements)

References 65 publications

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“…Microfabricated well arrays have been proposed to trap cells before nanoindentation [113][114][115]. This would enable automatic indentation for high-throughput screening methods that may prove useful for cells sorting.…”

Section: A Special Case: Cell Trapped Inside a Wellmentioning

confidence: 99%

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Nanobiomechanics of living cells: a review

Chen

2014

Interface Focus.

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Nanobiomechanics of living cells is very important to understand cellmaterials interactions. This would potentially help to optimize the surface design of the implanted materials and scaffold materials for tissue engineering. The nanoindentation techniques enable quantifying nanobiomechanics of living cells, with flexibility of using indenters of different geometries. However, the data interpretation for nanoindentation of living cells is often difficult. Despite abundant experimental data reported on nanobiomechanics of living cells, there is a lack of comprehensive discussion on testing with different tip geometries, and the associated mechanical models that enable extracting the mechanical properties of living cells. Therefore, this paper discusses the strategy of selecting the right type of indenter tips and the corresponding mechanical models at given test conditions.

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Section: A Special Case: Cell Trapped Inside a Wellmentioning

confidence: 99%

“…For example, finite-element simulations have been used to do inverse analysis to obtain the mechanical properties of cytoplasm [2,51,54], nucleus elastic properties [2,51,54] or the pericellular matrix [114]. As living materials, the external force may trigger passive stress generation in the cell.…”

Section: Inverse Finite-element Analysismentioning

confidence: 99%

Nanobiomechanics of living cells: a review

Chen

2014

Interface Focus.

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“…Fibers containing pristine microcrystals were compared to those containing sonicated microcrystals as well as those that had been either partially or extensively disassembled by lyophilization ( Figure S2). In order to convert the force versus displacement data to elastic modulus values, we applied the nonlinear Hertzian contact model ( Figure S7) 27 corrected for indentation geometry by finite element analysis ( Figure S8). Indeed, there is a dose-dependent increase in average fiber modulus, with the highest QL4 loading (8%) leading to the highest modulus values for each fiber type.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Mechanical Reinforcement of Polymeric Fibers through Peptide Nanotube Incorporation

et al. 2013

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High aspect ratio nanotubular assemblies can be effective fillers in mechanically reinforced composite materials. However, most existing nanotubes used for structural purposes are limited in their range of mechanical, chemical, and biological properties. We demonstrate an alternative approach to mechanical reinforcement of polymeric systems by incorporating synthetic D,L-cyclic peptide nanotube bundles as a structural filler in electrospun poly D-, L-lactic acid fibers. The nanotube bundles self-assemble through dynamic hydrogen bonding from synthetic cyclic peptides to yield structures whose dimensions can be altered based on processing conditions, and can be up to hundreds of micrometers long and several hundred nanometers wide. With 8 wt % peptide loading, the composite fibers are >5-fold stiffer than fibers composed of the polymer alone, according to atomic force microscopy-based indentation experiments. This represents a new use for self-assembling cyclic peptides as a load-bearing component in biodegradable composite materials.

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“…As previously described, Ng et al [2007] recently fabricated microwells that are capable of holding chondrons in place during AFM experimentation, thereby providing a potentially significant experimental modality to be used on single chondrons. In addition, other techniques may employ a type of matrix glue to attach the chondrons onto a given substrate prior to mechanical testing.…”

Section: Discussionmentioning

confidence: 99%

Micromechanical properties of chondrocytes and chondrons: relevance to articular cartilage tissue engineering

Ofek

Athanasiou

2007

JOMMS

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Articular cartilage is a highly mechanical tissue, performing multiple functions to ensure proper joint movement. Degradation of this tissue may be due to improper loading conditions that lead to a debilitating condition known as osteoarthritis. Furthermore, it is believed that mechanical signals transmitted from the tissue to cellular levels are necessary for the production of essential extracellular matrix components responsible for cartilage viability. Examinations of the tissue on its most rudimentary level elucidate mechanical regimens related to cartilage health and disease. A fundamental unit approach has been employed to study the biomechanical properties of single cells with discrete pericellular and extracellular matrix layers. This approach enables researchers to develop definitive relationships between mechanical stimulation and changes in gene expression corresponding to regenerative or catabolic processes. The knowledge gained from these studies sheds light on the etiology of osteoarthritis and elucidate the mechanical loading regimens useful for promoting articular cartilage health. This review article discusses the micromechanical environment of the cartilage cell, the chondrocyte, and the mechanical models and experimental techniques utilized to examine its physical characteristics. This information is then related to changes in cellular behavior and its potential toward tissue engineering of articular cartilage.

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Nanomechanical properties of individual chondrocytes and their developing growth factor-stimulated pericellular matrix

Cited by 81 publications

References 65 publications

Nanobiomechanics of living cells: a review

Nanobiomechanics of living cells: a review

Mechanical Reinforcement of Polymeric Fibers through Peptide Nanotube Incorporation

Micromechanical properties of chondrocytes and chondrons: relevance to articular cartilage tissue engineering

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