Nanomedicine: Interaction of biomimetic apatite colloidal nanoparticles with human blood components

Abstract

This contribution investigates the interaction of two types of biomimetic-apatite colloidal nanoparticles (negatively-charged 47nm, and positively-charged 190nm NPs) with blood components, namely red blood cells (RBC) and plasma proteins, with the view to inspect their hemocompatibility. The NPs, preliminarily characterized by XRD, FTIR and DLS, showed low hemolysis ratio (typically lower than 5%) illustrating the high compatibility of such NPs with respect to RBC, even at high concentration (up to 10mg/ml). The presence of glucose as water-soluble matrix for freeze-dried and re-dispersed colloids led to slightly increased hemolysis as compared to glucose-free formulations. NPs/plasma protein interaction was then followed, via non-specific protein fluorescence quenching assays, by contact with whole human blood plasma. The amount of plasma proteins in interaction with the NPs was evaluated experimentally, and the data were fitted with the Hill plot and Stern-Volmer models. In all cases, binding constants of the order of 10(1)-10(2) were found. These values, significantly lower than those reported for other types of nanoparticles or molecular interactions, illustrate the fairly inert character of these colloidal NPs with respect to plasma proteins, which is desirable for circulating injectable suspensions. Results were discussed in relation with particle surface charge and mean particle hydrodynamic diameter (HD). On the basis of these hemocompatibility data, this study significantly complements previous results relative to the development and nontoxicity of biomimetic-apatite-based colloids stabilized by non-drug biocompatible organic molecules, intended for use in nanomedicine.

Apatite nanoparticles strongly improve red blood cell cryopreservation by mediating trehalose delivery via enhanced membrane permeation

“…Although slightly larger than for incubation in physiological NaCl solution, these results indicate that the apatite NP studied here are biocompatible with regard to RBC and suitable for applications in blood transfusion. These findings agree well with recent studies where the good hemocompatibility of apatite NP was reported, especially concerning direct contact with RBC [31,33].…”

“…1b). Based on this hypothesis, and taking into account good hemocompatibility of colloidal apatite NP [33], the aim of the present work was to investigate, for the first time, the possibility of using AEP/HMP stabilized colloidal apatite nanoparticles for facil itating the delivery of trehalose into RBC, in view of promoting their cryopreservation without the use of glycerol. This paper reports experimental data on the role of colloidal apatite NP on RBC viability after freeze thawing cycle and explores, at the molecular scale and by using synthetic lipid bilayers, the mechanism of in teractions between RBC and apatite NP.…”

Biophytum sensitivum nanomedicine reduces cell viability and nitrite production in prostate cancer cells

“…There is no aggregation monitored in WBCs, RBCs, and platelets suggesting the level of interaction between plasma proteins and our BS extract are proven aspect to consider for inspecting haemocompatibility. Our data BS intended for use in contact with blood and interaction with plasma proteins which is supporting with bioavailability of NPs alteration and phagocytosis mechanism [48,49].…”

Interaction of Nanoparticles with Blood Components and Associated Pathophysiological Effects

“…Nanomaterial interaction with these cells has different effects depending on their intrinsic characteristics. Venkatesan et al [20] and Choimet et al [21] recently reported the high hemocompatibility of chitosan nanoparticles loaded with siRNA-Npr3 and nanoparticles formed with colloidal apatite; however, chitosan nanoparticles dissolved with tripolyphosphate (TPP) to acid pH produce hemolysis [22]. Kim et al [23] carried out rheological measurements and showed that, at concentrations of 12.5 μg/mL, silica nanomaterials caused hemolysis, deformation, and aggregation of erythrocytes.…”