NanoMOFs with Encapsulated Photosensitizer: Accumulation inChlamydia trachomatisInclusions and Antimicrobial Effects

Abstract

Metal-organic framework nanoparticles (nanoMOFs) are a promising class of hybrid nanomaterials for biomedical applications. Some of them, including biodegradable porous iron carboxylates are proposed for encapsulation and delivery of antibiotics. Due to the high drug loading capacity and fast internalization kinetics nanoMOFs are more beneficial for the treatment of intracellular bacterial infections compared to free antibacterial drugs, which poorly accumulate inside the cells because of the inability to cross membrane barriers or have low intracellular retention. However, nanoparticle internalization does not ensure their accumulation in the cell compartment that shelters a pathogen. This study shows the availability of MIL-100(Fe) MOF nanoparticles to co-localize with Chlamydia trachomatis, an obligate intracellular bacterium, in the infected RAW264.7 macrophages. Furthermore, nanoMOFs loaded with photosensitizer methylene blue (MB) exhibit complete photodynamic inactivation of Chlamydia trachomatis growth. Simultaneous infection and treatment of RAW264.7 cells with empty nanoMOFs resulted in a 3-fold decrease in bacterial load that indicates an intrinsic anti-chlamydial effect of this iron-containing nanomaterial. Thus, our findings suggest the use of iron-based nanoMOFs as a promising drug delivery platform, which contributes to antibacterial effect, for the treatment of chlamydial infections.

Metal-Organic Framework-Based Nanomedicines for the Treatment of Intracellular Bacterial Infections

“…The use of nanoparticles as DDS for the treatment of chlamydial infections is an attractive strategy. Recently, we showed the feasibility of MIL-100(Fe) nanoMOFs to co-localize with Ctr in the infected RAW264.7 macrophages ( Figure 4 B) [ 103 ]. In the same study, a photodynamic strategy was used to eradicate Ctr using photosensitizer-loaded nanoMOFs that could find a potent application for the treatment of mucosal infections.…”

“…It has been revealed that the nanoMOF that encapsulated the photosensitizer methylene blue (MB) exhibited significant photodynamic inactivation of Ctr in infected cells resulting in a two order of magnitude bacterial burden decrease in comparison with the non-treated infected control and more than a one order of magnitude greater Ctr inhibition in comparison with free MB. Interestingly, it was found that MIL-100(Fe) nanoMOFs have an intrinsic anti-chlamydial effect presumably due to involvement into a Fenton reaction [ 103 ]. Thus, our findings suggest the use of iron(III)-based nanoMOFs as a promising drug delivery nanoplatform, which contributes to antibacterial effect, for the treatment of mucus chlamydial infections by local administration.…”