Nanoparticle-based non-viral CRISPR delivery for enhanced immunotherapy

Shin, Heungsoo; Kim, Jaeyun

doi:10.1039/d1cc05999h

Cited by 7 publications

(3 citation statements)

References 110 publications

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“…In prokaryotes, the CRISPR-Cas system confers resistance to foreign plasmids and phage sequences and recognizes and silences invading functional elements, thereby acting as the immune system ( Dong et al, 2022 ; Shin and Kim, 2022 ). Types I, II, III, IV, V, and VI of CRISPR systems have been reported so far ( Wright et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative genomic analysis of Lacticaseibacillus paracasei SMN-LBK from koumiss

Wang

Wang²,

Li³

et al. 2022

Front. Microbiol.

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Lacticaseibacillus paracasei SMN-LBK, which was isolated in Xinjiang, has been shown to be a probiotic strain and used as the auxiliary starter for dairy fermentation. Comparative genomic analysis was performed to investigate the metabolic preference and ethanol tolerance mechanisms of L. paracasei SMN-LBK. The results of comparative genomics showed that L. paracasei strains had high conservation and genetic diversity. SMN-LBK encoded various genes related to carbohydrate and amino acid metabolism pathways, which endow this strain with good fermentation potential. In addition, 6 CRISPR sequences and 8 cas proteins were found in SMN-LBK, and these could play vital roles in the immune system. Furthermore, a unique cluster of potential secondary metabolism genes related to bacteriocins was detected in the genome of SMN-LBK, and this could be important for the preservation of fermented foods. Multiple genes related to alcohol tolerance were also identified. In conclusion, our study explained the traits that were previously demonstrated for SMN-LBK as phenotypes and provided a theoretical basis for the application of SMN-LBK in the food industry.

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Section: Discussionmentioning

confidence: 99%

Comparative genomic analysis of Lacticaseibacillus paracasei SMN-LBK from koumiss

Wang

Wang²,

Li³

et al. 2022

Front. Microbiol.

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“…These vehicles can be broadly categorized into natural EVs, modified EVs, VLPs, and exosome-liposome hybrid vesicles. Due to the potential safety concerns associated with viral delivery systems, such as high immunogenicity and the risk of viral gene integration, 91 non-viral CRISPR delivery systems are gaining prominence. These systems offer significant advantages, including high loading capacity, stability, and biocompatibility, thereby ensuring safe and efficient delivery.…”

Section: Comparison Of Different Evsmentioning

confidence: 99%

Functionalized extracellular nanovesicles as advanced CRISPR delivery systems

Wang,

Kong,

Zhuo

et al. 2024

Biomater. Sci.

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“…So far, various types of NPs including gold NPs, polymer NPs, lipid NPs, peptide NPs, and DNA nanostructures have been employed to deliver the CRISPR/Cas9 system. In general, in CRISPR designs, NPs have outstanding advantages such as easy synthesis, high loading capacity, in vitro and in vivo applicability, low cost, and high efficiency [ 26 – 33 ]. In this study, Bovine Serum Albumin (BSA) coated with polyethylenimine (PEI) was used to deliver CRISPR/Cas9 system in plasmid and ribonucleoprotein (RNP) forms into the MDA-MB-231 cells; as in this structure PEI binds to the surface of BSA through electrostatic interactions.…”

Section: Introductionmentioning

confidence: 99%

BSA-PEI Nanoparticle Mediated Efficient Delivery of CRISPR/Cas9 into MDA-MB-231 Cells

et al. 2022

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The discovery of bacterial-derived Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has revolutionized genome engineering and gene therapy due to its wide range of applications. One of the major challenging issues in CRISPR/Cas system is the lack of an efficient, safe, and clinically suitable delivery of the system’s components into target cells. Here, we describe the development of polyethylenimine coated-bovine serum albumin nanoparticles (BSA-PEI NPs) for efficient delivery of CRISPR/Cas9 system in both DNA (px458 plasmid) and ribonucleoprotein (RNP) forms into MDA-MB-231 human breast cancer cell line. Our data showed that synthesized BSA-PEI (BP) NPs delivered plasmid px458 at concentrations of 0.15, 0.25, and 0.35 µg/µl with efficiencies of approximately 29.7, 54.8, and 84.1% into MDA-MB-231 cells, respectively. Our study demonstrated that Cas9/sgRNA RNP complex efficiently (~ 92.6%) delivered by BSA-PEI NPs into the same cells. Analysis of toxicity and biocompatibility of synthesized NPs on human red blood cells, MDA-MB-231 cells, and mice showed that the selected concentration (28 µg/µl) of BSA-PEI NPs for transfection had no remarkable toxicity effects. Thus, obtained results suggest BSA-PEI NPs as one of the most promising carrier for delivering CRISPR/Cas9 to target cells.

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Nanoparticle-based non-viral CRISPR delivery for enhanced immunotherapy

Abstract: The CRISPR Cas9 system has received considerable attention due to its simplicity, efficiency, and high precision for gene editing. The development of various therapeutic applications of the CRISPR system is...

Cited by 7 publications

References 110 publications

Comparative genomic analysis of Lacticaseibacillus paracasei SMN-LBK from koumiss

Comparative genomic analysis of Lacticaseibacillus paracasei SMN-LBK from koumiss

Functionalized extracellular nanovesicles as advanced CRISPR delivery systems

BSA-PEI Nanoparticle Mediated Efficient Delivery of CRISPR/Cas9 into MDA-MB-231 Cells

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