Nanoparticle binding attenuates the pathobiology of gastric cancer-associatedHelicobacter pylori

Abstract

Enteric bacteria may cause severe diseases, including gastric cancer-associated Helicobacter pylori. Their infection paths overlap with the oro-gastrointestinal uptake route for nanoparticles, increasingly occurring during environmental or consumer/medical exposure. By comprehensive independent analytical methods, such as live cell fluorescence, electron as well as atomic force microscopy and elemental analysis, we show that a wide array of nanoparticles (NPs) but not microparticles form complexes with H. pylori and enteric pathogens without the need for specific functionalization. The NP-assembly that occurred rapidly was not influenced by variations in physiological temperature, though affected by the NPs' physico-chemical characteristics. Improved binding was observed for small NPs with a negative surface charge, whereas binding could be reduced by surface 'stealth' modifications. Employing human gastric epithelial cells and 3D-organoid models of the stomach, we show that NP-coating did not inhibit H. pylori's cellular attachment. However, even the assembly of non-bactericidal silica NPs attenuated H. pylori infection by reducing CagA phosphorylation, cytoskeletal rearrangement, and IL-8 secretion. Here we demonstrate that NP binding to enteric bacteria may impact their pathobiology which could be further exploited to rationally modulate the (patho)biology of microbes by nanomaterials.

Bioelectricity, Its Fundamentals, Characterization Methodology, and Applications in Nano‐Bioprobing and Cancer Diagnosis

“…Several studies reported on the effects of nanoparticles on the surface properties of bacteria and bio‐behaviors in terms of nanoparticles shape, size, and surface charge, Lyden studied the strong electrostatic interaction between hyphae and carboxylate‐functionalized nanoparticles . As shown in Figure 9 A,B, the surface‐absorbed nanoparticles on C. albicans hyphae is evident via electrostatic interaction.…”

Boosting nanotoxicity to combat multidrug-resistant bacteria in pathophysiological environments

“…We tested NMs varying in size and/or surface functionalization, including NMs that have been proven as reliable models in previous studies, to dene determinants for bacteria-NM interactions and antibiotic effects (Table 1). 13,35,[55][56][57][58] For one, we rst analyzed the interaction of various uorescent polymer and silica NMs with auto-uorescent pathogenic as well as apathogenic bacterial genera in situ using mainly live cell microscopy as a non-invasive method (Fig. 1a, b and e).…”

“…Silica, polymer, and metal-based NMs as well as various microparticles were synthesized and provided by the DENANA/ NanoBEL Research consortium, purchased (Merck, Invitrogen, MSC UG) or synthesized as indicated. 22,29,35,[55][56][57][80][81][82] NMs were characterized by EM, DLS, and zeta potential measurements as reported. 55,57,83 Clinical isolates and cultivation of bacteria MDR bacteria were isolated and respective resistance phenotypes characterized at the University Medical Centers of Essen and Mainz using microbial methods as described.…”

“…22,29,35,[55][56][57][80][81][82] NMs were characterized by EM, DLS, and zeta potential measurements as reported. 55,57,83 Clinical isolates and cultivation of bacteria MDR bacteria were isolated and respective resistance phenotypes characterized at the University Medical Centers of Essen and Mainz using microbial methods as described. 22,32,33,81 Identity conrmation and susceptibility testing were performed by the semi-automated systems VITEK 2 (bioMérieux, Germany) and MicroScan WalkAway (Siemens, Germany) or by MALDI Biotyper® System (Bruker, Germany).…”

Toxico‐Metabolomics of Engineered Nanomaterials: Progress and Challenges

“…Due to the complicated mutualistic relationships between microbes and their human host, recent changes in human lifestyles due to a greater incorporation of nanotechnology has put significant pressure on the consortia of gut microbiota . Therefore, the exposure to ENMs ( Table 2 ) may modify the composition and diversity of the consortia of the microbiome . The exposure to ZnO NPs (30 nm) for 9 weeks at the dietary dose of 100 mg kg ‐1 altered the richness of the bacterial community and significantly reduced the Lactobacillus in the ileal digesta and plasma metabolites of hens.…”