Nanoparticles Complexed with Gene Vectors to Promote Proliferation of Human Vascular Endothelial Cells

Abstract

Amphiphilic block copolymers containing biodegradable hydrophobic segments of depsipeptide based copolymers have been synthesized and explored as gene carriers for enhancing proliferation of endothelial cells in vitro. These polymers form nanoparticles (NPs) with positive charges on their surface, which could condense recombinant plasmids of enhanced green fluorescent protein plasmid and ZNF580 gene (pEGFP-ZNF580) and protect them against DNase I. ZNF580 gene is efficiently transported into EA.hy926 cells to promote their proliferation, whereby the transfection efficiency of NPs/pEGFP-ZNF580 is approximately similar to that of Lipofectamine 2000. These results indicate that the NPs might have potential as a carrier for pEGFP-ZNF580, which could support endothelialization of cardiovascular implants.

Electrospun Poly(lactide-co-glycolide-co-3(S)-methyl-morpholine-2,5-dione) Nanofibrous Scaffolds for Tissue Engineering

“…The main reason was the degradation product (L-alanine) of MMD in P(LA-co-GA-co-MMD) copolymers might promote the proliferation of HUVECs via a signal pathway [43]. These results were in good agreement with our previous studies [42,46]. MMD) nanofibrous scaffolds increased with the increasing contents of MMD in copolymers and HUVECs on the P(LA-co-GA-co-MMD)3 scaffolds exhibited the highest relative cell viability.…”

“…Moreover, the degradation products of MMD including L-amino acids can stimulate the proliferation of endothelial cells via a signal pathway and be properly metabolized by living tissues [42][43][44][45]. Based on the copolymers of MMD with other cyclic monomers, our group have developed some gene delivery systems for promoting the transfection and migration of endothelial cells [42,[46][47][48][49]. However, to the best of our knowledge, the study on electrospun nanofibrous scaffolds from the copolymers of MMD with other cyclic monomers for vascular tissue engineering has not been reported previously.…”

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

“…22 In our previous studies, several gene carriers to condense with pEGFP-ZNF580 gene (pEGFP-ZNF580 gene is the recombinant plasmid of enhanced GFP plasmid and ZNF580 gene) were prepared with different block copolymers for the proliferation of ECs. [23][24][25][26][27][28][29] As the "gold" standard nonviral gene carrier, polyethylenimine (PEI) is widely used as an effective gene carrier, which exhibits a good performance in condensing DNA due to its high positive charge density. 30 However, the drawbacks of high-molecular weight PEI, including nondegradability and high cytotoxicity, limit its application in vivo.…”

Amphiphilic core shell nanoparticles containing dense polyethyleneimine shells for efficient delivery of microRNA to Kupffer cells

“…[13][14][15][16][17][18] For example, Lv et al reported that a complex micelle consisting of a biodegradable PLGA core and a mixed PEG/PEI shell can be prepared via the self-assembly of two block copolymers (PEG-b-PLGA and PLGA-b-PEI) in aqueous solution. This micelle can be used as a gene carrier for enhancing proliferation of endothelial cells.…”

“…17 Recently, Li et al developed a new kind of gene carrier based on the amphiphilic block copolymers containing biodegradable hydrophobic depsipeptide copolymers and branched PEI. 18 We have also developed a novel type of PEI-based amphiphilic core-shell nanoparticles (NPs) with diameters in the range of 100-300 nm and explored their potential application as nanocarriers for drug and gene deliveries. [19][20][21] Compared with the abovementioned micellarbased biodegradable NPs, the PEI-based core-shell NPs possess several distinct advantages: 1) PEI@poly(methyl methacrylate) (PMMA) core-shell NPs with adjustable diameters ranging from 100 nm to 300 nm can be easily synthesized and scaled up; 2) the PEI shells allow rapid and efficient encapsulation of DNA molecules through the electrostatic complexation; 3) the immobilized PEI is three to four times less toxic than the native free PEI and is at least threefold more efficient as gene carriers in transfecting cells; 4) the amount of PEI needed to form complexed NPs with DNA molecules can be significantly reduced when using preformed uniform core-shell NPs containing PEI shells; and 5) the resulting DNA/NP complexes are highly uniform and homogeneous, providing good delivery system for pharmacokinetic studies.…”