Nanoparticles of Lyotropic Liquid Crystals: A Novel Strategy for the Topical Delivery of a Chlorin Derivative for Photodynamic Therapy of Skin Cancer

Petrilli, Raquel; Praça, Fabíola Silva Garcia; Carollo, Aline Regina Hellmann; Medina, Wanessa Silva Garcia; Oliveira, Kléber T. de; Fantini, M.C.A.; Neves, Maria G. P. M. S.; Cavaleiro, José A. S.; Serra, Osvaldo Antônio; Iamamoto, Yassuko; Bentley, Maria Vitória Lopes Badra

doi:10.2174/1573413711309040003

Cited by 25 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, herein the use of human skin was avoided and the purpose of this study was to provide a comparison of various animal skin types that could serve as skin membrane models for in-vitro penetration studies. The use of porcine ear skin, hairless mouse skin and shed snake skin has been widely studied as animal model membranes to replace human skin (Vecchia and Bunge, 2005;Itoh et al, 1990a;Itoh et al, 1990b;Rigg and Barry, 1990) as well as to evaluate skin permeation enhancers (Campos et al, 2016;Petrilli et al, 2013;Praça et al, 2012;Baby et al, 2008;Lopes et al, 2007;Nunes et al, 2005;Nicolazzo et al, 2003;Hirvonen et al, 1991), once they are easily available, easy to employ and can provide results rapidly (Jung and Maibach, 2015). Porcine ear skin and hairless mouse skin are widely employed, while shed snake skin has also potential to be used as a membrane model based on stratum corneum (SC) penetration rate (Praça et al, 2012;Baby et al, 2008;Nunes et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models

Praça

Medina

Eloy

et al. 2018

European Journal of Pharmaceutical Sciences

Self Cite

View full text Add to dashboard Cite

In vitro skin permeation/penetration studies may be affected by many sources of variation. Herein, we aimed to investigate the major critical procedures of in vitro skin delivery studies. These experiments were performed with model drugs according to official guidelines. The influence of skin source on penetration studies was studied as well as the use of a cryopreservation agent on skin freezing evaluated by transepidermal water loss, electrical resistance, permeation/penetration profiles and histological changes of the skin. The best condition for tape stripping procedure was validated through the evaluation of the distribution of corneocytes, mass of stratum corneum (SC) removed and amount of protein removed using finger pressure, a 2kg weight and a roller. The interchangeability of the tape stripping procedures followed by the epidermis and dermis homogenate and the micrometric horizontal cryostat skin sectioning methods were also investigated, besides the effect of different formulations. Noteworthy, different skin sources were able to ensure reliable interchangeability for in vitro permeation studies. Furthermore, an increased penetration was obtained for stored frozen skin compared to fresh skin, even with the addition of a cryoprotectant agent. The best method for tape stripping was the finger pressure followed by the addition of a propylene glycol solvent leading to better SC removal. Finally, no significant difference was found in skin penetration studies performed by different methods suggesting their possible interchangeability.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models

Praça

Medina

Eloy

et al. 2018

European Journal of Pharmaceutical Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…[1][2][3] The polymorphism of MO self-assembly has been studied extensively. Self-assembled MO nds application as carriers in drug delivery, [6][7][8][9][10][11][12][13] and in gene therapy, [14][15][16] cancer therapy, [17][18][19][20] protein crystallisation, [21][22][23][24][25][26] and MRI imaging. 1).…”

Section: Introductionmentioning

confidence: 99%

Nanostructure and cytotoxicity of self-assembled monoolein–capric acid lyotropic liquid crystalline nanoparticles

et al. 2015

View full text Add to dashboard Cite

Monoolein forms self-assembled nanoparticles with various internally ordered nanostructures, including the lyotropic liquid crystalline inverse hexagonal and inverse bicontinuous cubic phases. This study investigated the influence of a saturated fatty acid, capric acid (decanoic acid), on the formation of different lyotropic liquid crystalline phases in monoolein-based systems. The nanoparticles were characterized by synchrotron small angle X-ray scattering (SAXS), cryogenic transmission electron microscopy (cryo-TEM), dynamic light scattering, and zeta potential measurements. The addition of capric acid to monoolein triggered concentration dependent phase changes with the sequence evolving from an inverse primitive cubic phase to inverse double-diamond cubic, inverse hexagonal (H II ), and emulsified microemulsions. SAXS and cryo-TEM revealed the formation of both single phase and mixed phases within a nanoparticle. To understand the cytotoxicity effects of the different nanoparticles, cellular cytotoxicity and hemolysis assays were performed. Nanoparticles in emulsion and hexagonal phases were found to be less toxic than cubic phase nanoparticles. The hemolysis assays followed the same trend with cubic phase dispersions causing the highest level of hemoglobin release. In summary, this study showed that the internal lyotropic liquid crystal mesophase structure of self-assembled nanoparticles needs careful consideration in the design of drug delivery vehicles. ; Tel: +61 3 9925 4265 † Electronic supplementary information (ESI) available. See

show abstract

“…Another interesting application of lyotropic liquid crystal nanoparticles is for photodynamic therapy (PDT) [85,86]. In PDT, a photosensitizer that can be activated by insertion into the plasma membrane bilayer was visualized.…”

Section: Cryo-tem Of Vitreous Sections (Cemovis)mentioning

confidence: 99%

“…Incorporation of photosensitizers in nanoparticles is one of the strategies to overcome this difficulty. Petrilli et al[85] investigated in-vitro and in-vivo penetration photosensitizer Chlorin activated nanoparticles in animal models and confirmed that the nano-dispersion of hexagonal phase enables a higher drug skin uptake compared to the control. They demonstrated a higher bio-distribution of the chlorin derivative in the skin layers of hairless mice compared to the control.…”

mentioning

confidence: 93%

Liquid crystal nanoparticles for commercial drug delivery

Milleret

Nagaraj

2017

Liquid Crystals Reviews

View full text Add to dashboard Cite

show abstract

Nanoparticles of Lyotropic Liquid Crystals: A Novel Strategy for the Topical Delivery of a Chlorin Derivative for Photodynamic Therapy of Skin Cancer

Cited by 25 publications

References 0 publications

Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models

Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models

Nanostructure and cytotoxicity of self-assembled monoolein–capric acid lyotropic liquid crystalline nanoparticles

Liquid crystal nanoparticles for commercial drug delivery

Contact Info

Product

Resources

About