Nanoparticulate lipid dispersions for bromocriptine delivery: Characterization and in vivo study

Esposito, Elisabetta; Mariani, Paolo; Ravani, Laura; Contado, Catia; Volta, Mattia; Bido, Simone; Drechsler, Markus; Mazzoni, Serena; Menegatti, Enea; Morari, Michele; Cortesi, Rita

doi:10.1016/j.ejpb.2011.10.015

Cited by 114 publications

(58 citation statements)

References 48 publications

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“…The aliquots of 20 ml of each sample were injected and analysed at 305 nm. [19] The standard curve was plotted in the concentration range of 0.0078À5mg/ml. The retention time for BCT was 4.5 min and the lowest detection limit was found to be 0.0078 mg/ml.…”

Section: Hplc Analysis Of Bctmentioning

confidence: 99%

“…[18] Esposito et al demonstrated the potential of lipid nanocarriers to improve the therapeutic effects of the BCT for Parkinson's disease. [19] Chitosan gel mediated delivery of BCT revealed the effectiveness of transdermal route as an alternate to oral delivery. [20] Literature suggests the application of transdermal delivery system to improve the therapeutic efficacy of BCT by avoiding hepatic metabolism and manitaining optimal plasma concentrations for a long period.…”

Section: Introductionmentioning

confidence: 99%

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Proniosomal gel-mediated transdermal delivery of bromocriptine:in vitroandex vivoevaluation

Lather¹,

Sharma²,

Pandita³

2016

Journal of Experimental Nanoscience

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Vesicular systems endow large opportunities for the transdermal delivery of therapeutics. The present study was designed to investigate the potential of a novel class of vesicular system 'proniosome' as a carrier for transdermal delivery of bromocriptine (BCT). Proniosome formulations were prepared by the coacervationphase separation method and the influence of factors like surfactant type and its amount, lipid concentration, cholesterol amount and drug content were studied. Span 60 was the most appropriate surfactant, and yielded vesicle size and percentage encapsulation efficiency of 1.3 mm and 98.9%, respectively. The developed system was characterised w.r.t. morphology, transition temperature, drug release, skin permeation and skin irritancy. Proniosomes exhibited a sustained release pattern of BCT in vitro. Skin permeation study revealed high penetration of proniosomes with sustained release of BCT through rat skin. The optimised proniosomal formulation showed enhanced transdermal flux of 16.15 mg/cm 2 /h as compared to 3.67 mg/cm 2 /h for drug dispersion. The developed formulations were observed as non-irritant to the rat skin and were found as quite stable at 4 and 25 C for 90 days w.r.t. vesicle size and drug content. The dried proniosomal formulation could act as a promising alternative to niosomes and preferably for transdermal delivery of BCT.

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Section: Hplc Analysis Of Bctmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proniosomal gel-mediated transdermal delivery of bromocriptine:in vitroandex vivoevaluation

Lather¹,

Sharma²,

Pandita³

2016

Journal of Experimental Nanoscience

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“…Therefore only the amounts of trackers needed for the specific determinations were included. Nevertheless, previous studies from our research group demonstrated that different drugs could be entrapped in lipid nanoparticles in amounts higher enough to exert a therapeutic effect [13,17,27].…”

Section: Preparation Of Lipid Nanoparticlesmentioning

confidence: 99%

“…Cryogenic transmission electron microscopy (cryo-TEM) analysis: Samples were vitrified as previously described [13]. The vitrified specimen was transferred to a Zeiss EM922Omega transmission electron microscope for imaging using a cryoholder (CT3500, Gatan).…”

Section: Sedimentation Field Flow Fractionation (Sdfff) Analysismentioning

confidence: 99%

Production, Physico-Chemical Characterization and Biodistribution Studies of Lipid Nanoparticles

Esposito¹

2015

J Nanomed Nanotechnol

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This study describes the preparation, characterization, in vitro uptake and in vivo biodistribution in mice of solid lipid nanoparticles and nanostructured lipid carriers. Biodistribution of solid lipid nanoparticles treated by polysorbate 80 was evaluated by fluorescent luminescent imaging after intraperitoneal administration in mice. The in vivo images indicate that nanoparticles were able to reach the brain, even if they prevalently accumulated in liver and spleen.

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“…Considering the properties of biodegradable NPs essentially grand bioavailability, control release, less toxicity and better encapsulation, they are frequently used as a vehicle in drug delivery. Biodegradable polymers such as methoxy polyethylene glycol-poly (caprolactone) (MPEG-PCL), poly lactic-co-glycolic acid, poly lactic acid, chitosan, gelatin, polycaprolactone, and poly-alkylcyanoacrylales are used for nanoformulations [6,7]. MPEG-PCL which possesses the idiosyncratic features such as non-toxicity, hydrophilicity, biodegradability, mechanical strength, biocompatibility, and physical inertness used as a carrier for targeted drug delivery in neurodegenerative disorder [8,9].…”

Section: Introductionmentioning

confidence: 99%

Pramipexole Dihydrochloride Loaded Mpegpcl Nanosuspension by Modified Nanoprecipitation: In Vitro and in Vivo Evaluation

Sundaram

2016

Asian J Pharm Clin Res

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Objective: Nanosuspension in drug delivery is known to improve solubility, dissolution, and eventually bioavailability of the drugs in the brain. This study was carried out to show the neuroprotective effect of pramipexole dihydrochloride nanosuspension for the potential treatment in Parkinson's disease and to evaluate the effectiveness in both in vitro and in vivo models.Methods: Nanosuspension of pramipexole dihydrochloride was prepared with methoxy polyethylene glycol-poly (caprolactone) (MPEG-PCL) and Pluronic F68 by the process of modified nanoprecipitation technique with different concentrations of MPEG-PCL. The particle size, zeta potential, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and in vitro drug release were performed. The cell viability study was performed using SH-SY5Y cells. Further, the formulation is evaluated for its antioxidant potential against rotenone-induced neuronal damage in male Wister rats such as enzymatic, non-enzymatic antioxidants, and histopathological evaluation.Result: The nanoformulation shows least particle size of 143 nm and maximum zeta potential value of 33.4 mv with 88.53% entrapment efficiency were observed with PMPNP 2 formulation. The SEM, TEM, and in vitro drug release of PMPNP 2 were showed spherical shape with controlled release when compared to other formulations. Further, the MTT assay was performed using SH-SY5Y cells which show more than 50% cell viability with 50 μl of PPMNP 2 nanoformulation. Moreover, the antioxidant potential done in rotenone-induced neuronal damage in Wister rats. The results showed elevation in the levels of enzymatic and non-enzymatic antioxidants compared with the neuronal toxic group, and the nanoformulation group showed a decrease in levels of lipid peroxidation which correlates with histopathological architecture. Conclusion:This study concluded that our nanoformulation shows a significant advantage of neuroprotective effect than the plain formulation for the treatment of Parkinson's disease in both in vitro and in vivo studies.

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Nanoparticulate lipid dispersions for bromocriptine delivery: Characterization and in vivo study

Cited by 114 publications

References 48 publications

Proniosomal gel-mediated transdermal delivery of bromocriptine:in vitroandex vivoevaluation

Proniosomal gel-mediated transdermal delivery of bromocriptine:in vitroandex vivoevaluation

Production, Physico-Chemical Characterization and Biodistribution Studies of Lipid Nanoparticles

Pramipexole Dihydrochloride Loaded Mpegpcl Nanosuspension by Modified Nanoprecipitation: In Vitro and in Vivo Evaluation

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