Production, Physico-Chemical Characterization and Biodistribution Studies of Lipid Nanoparticles

Esposito, Elisabetta

doi:10.4172/2157-7439.1000256

Cited by 10 publications

(8 citation statements)

References 24 publications

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“…Indeed, the most important benefit of microscopic technique is that it is feasible to know about the morphology and the diameter of the particles within the same run. However, preparing the sample is very important—nanoparticles should cover the substrate precisely and reagents used in sample preparation should be appropriate [ 110 , 111 ]. In addition, it should be mentioned that microscopic techniques used for the characterization of nano-sized materials could be challenging if the sample to be analyzed is polydispersed due to aggregation.…”

Section: Morphological Characterization Of Nanoparticlesmentioning

confidence: 99%

“…In one study concerning lipid-based fluorescent nanoparticles designed to access in-vivo biodistribution via fluorescent luminescent imaging and in vitro uptake on hCMEC/D3 cell line, SdFFF was utilized to determine the size distribution of particles by converting the fractograms and it was useful to study the influence of different compositions on the overall diameter. For instance, authors were able to confirm that the lipid component and the presence of both fluorescent and tween 80 slightly affected nanoparticle dimensions [ 110 ]. Furthermore, the attained fractograms at fixed density values have been converted into PSD plots (the amount of material per unit change of diameter, according to well-verified equations) by transforming the retention time in a size.…”

Section: Size Measurementsmentioning

confidence: 99%

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Challenges in the Physical Characterization of Lipid Nanoparticles

et al. 2021

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Nano-sized drug transporters have become an efficient approach with considerable commercial values. Nanomedicine is not only limited to drug delivery by means of different administration routes, such as intravenous, oral, transdermal, nasal, pulmonary, and more, but also has applications in a multitude of areas, such as a vaccine, antibacterial, diagnostics and imaging, and gene delivery. This review will focus on lipid nanosystems with a wide range of applications, taking into consideration their composition, properties, and physical parameters. However, designing suitable protocol for the physical evaluation of nanoparticles is still conflicting. The main obstacle is concerning the sensitivity, reproducibility, and reliability of the adopted methodology. Some important techniques are compared and discussed in this report. Particularly, a comparison between different techniques involved in (a) the morphologic characterization, such as Cryo-TEM, SEM, and X-ray; (b) the size measurement, such as dynamic light scattering, sedimentation field flow fractionation, and optical microscopy; and (c) surface properties, namely zeta potential measurement, is described. In addition, an amperometric tool in order to investigate antioxidant activity and the response of nanomaterials towards the skin membrane has been presented.

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Section: Morphological Characterization Of Nanoparticlesmentioning

confidence: 99%

Section: Size Measurementsmentioning

confidence: 99%

Challenges in the Physical Characterization of Lipid Nanoparticles

et al. 2021

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“…Determining the particle size is of great importance in nanostructured formulations as the small particle size contributes to a greater interfacial area, which can then provide better drug partitioning and absorption at the skin surface. [47][48][49] Zeta potential Zeta potential (ZP) is a key factor for evaluation of the stability of nano dispersion. The ZP determination is based on particle electrophoretic mobility in aqueous medium.…”

Section: Particle Size Measurementmentioning

confidence: 99%

Nanostructured Lipid Carriers: A Groundbreaking Approach for Transdermal Drug Delivery

Chauhan¹,

Yasir

Verma³

et al. 2020

Adv Pharm Bull

293

145

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Nanostructured lipid carriers (NLCs) are novel pharmaceutical formulations which are composed of physiological and biocompatible lipids, surfactants and co-surfactants. Over time, as a second generation lipid nanocarrier NLC has emerged as an alternative to first generation nanoparticles. This review article highlights the structure, composition, various formulation methodologies, and characterization of NLCs which are prerequisites in formulating a stable drug delivery system. NLCs hold an eminent potential in pharmaceuticals and cosmetics market because of extensive beneficial effects like skin hydration, occlusion, enhanced bioavailability, and skin targeting. This article aims to evoke an interest in the current state of art NLC by discussing their promising assistance in topical drug delivery system. The key attributes of NLC that make them a promising drug delivery system are ease of preparation, biocompatibility, the feasibility of scale up, non-toxicity, improved drug loading, and stability.

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“…The separation of fluorescent nanoparticles from free fluorophores and the determination of fluorescence content of nanoparticles were made as previously reported. 30 Briefly, 300 µl of nanoparticle samples were loaded in a centrifugal filter (Microcon unit YM-10 membrane, NMWCO 10 kDa, Sigma) and subjected to centrifugation at 8,000 rpm for 60 min using a Spectrafuge™ 24D Digital Microcentrifuge (Woodbridge NJ, USA). Afterwards, the fluorescence in the eluate was determined by fluorescence spectroscopy, taking as reference a previously made calibration curve allowing the determination of the encapsulation efficiency (EE) and loading capacity (LC).…”

Section: Characterization Of Solid Lipid Nanoparticlesmentioning

confidence: 99%

<p>A Correlative Imaging Study of in vivo and ex vivo Biodistribution of Solid Lipid Nanoparticles</p>

Mannucci¹,

Boschi²,

Cisterna³

et al. 2020

IJN

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Solid lipid nanoparticles are largely used in biomedical research and are characterized by high stability and biocompatibility and are also able to improve the stability of various loaded molecules. In vitro studies demonstrated that these nanoparticles are low cytotoxic, while in vivo studies proved their efficiency as nanocarriers for molecules characterized by a low bioavailability. However, to our knowledge, no data on the systemic biodistribution and organ accumulation of solid lipid nanoparticles in itself are presently available. Methods: In this view, we investigated the solid lipid nanoparticles biodistribution by a multimodal imaging approach correlating in vivo and ex vivo analyses. We loaded solid lipid nanoparticles with two different fluorophores (cardiogreen and rhodamine) to observe them with an optical imager in the whole organism and in the excised organs, and with fluorescence microscopy in tissue sections. Light and transmission electron microscopy analyses were also performed to evaluate possible structural modification or damage due to nanoparticle administration. Results: Solid lipid nanoparticles loaded with the two fluorochromes showed good optic characteristics and stable polydispersity. After in vivo administration, they were clearly detectable in the organism. Four hours after the injection, the fluorescent signal occurred in anatomical districts corresponding to the liver and this was confirmed by the ex vivo acquisitions of excised organs. Brightfield, fluorescence and transmission electron microscopy confirmed solid lipid nanoparticles accumulation in hepatocytes without structural damage. Conclusion: Our results support the systemic biocompatibility of solid lipid nanoparticles and demonstrate their detailed biodistribution from the whole organism to organs until the cells.

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Production, Physico-Chemical Characterization and Biodistribution Studies of Lipid Nanoparticles

Cited by 10 publications

References 24 publications

Challenges in the Physical Characterization of Lipid Nanoparticles

Challenges in the Physical Characterization of Lipid Nanoparticles

Nanostructured Lipid Carriers: A Groundbreaking Approach for Transdermal Drug Delivery

<p>A Correlative Imaging Study of in vivo and ex vivo Biodistribution of Solid Lipid Nanoparticles</p>

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