Nanopatterned silk-coated AZ31 magnesium alloy with enhanced antibacterial and corrosion properties

Mehrjou, Babak; Dehghan-Baniani, Dorsa; Mo, Shi; Shanaghi, Ali; Wang, Guomin; Liu, Liangliang; Qasim, Abdul Mateen; Chu, Paul K.

doi:10.1016/j.msec.2020.111173

Cited by 26 publications

(17 citation statements)

References 60 publications

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“…However, release of Mg +2 results in poor mechanical properties and in the worst case, can lead to loosening of the implant. In fact, a high alkalinity is not suitable for cells and it is important to improve the corrosion properties of magnesium‐based alloys while not compromising the antibacterial properties or even enhancing them 14 in comparison with the results obtained by Ji et al 7,8 …”

Section: Discussionmentioning

confidence: 67%

“…After activation, the optical density of the bacteria was monitored at 600 nm and tuned to 0.1 (OD600 = 0.1) by adding the fresh medium and reactivation for 3 hr. To clean the surface from living organisms, the samples were exposed to ultraviolet (UV) light for about 1 hr before bacteria seeding which was performed according to the literature 11,14‐21 . The bacteria‐containing solution was diluted to (2–3) × 10 5 CFU ml −1 and 100 μl of the solution were spread on a cell culture dish without the sample as a blank and also on the untreated (bare) sample as the control sample.…”

Section: Methodsmentioning

confidence: 99%

“…To clean the surface from living organisms, the samples were exposed to ultraviolet (UV) light for about 1 hr before bacteria seeding which was performed according to the literature. 11,[14][15][16][17][18][19][20][21] The bacteria-containing solution was diluted to (2-3) Â 10 5 CFU ml À1 and 100 μl of the solution were spread on a cell culture dish without the sample as a blank and also on the untreated (bare) sample as the control sample. At time points of 6 and 24 hr, the colonies of E.coli were counted by the Image J software (USA) and examined by SEM.…”

Section: Bacteria and Cell Studiesmentioning

confidence: 99%

“…The MC3T3-E1 (Cell bank of the Chinese Academy of Sciences) osteoblast cell line was used in the cell study. According to the literature, 11,[14][15][16][17][18][19][20][21] 100 μl of the cell suspension with a density of about 20,000 MC3T3 ml À1 were seeded on the samples with 1 ml of the Dulbecco's modified eagle medium (DMEM) on a 24-well plate. After certain time intervals, the samples were examined by fluorescent microscopy, methylthialazole tetrazolium (MTT), and cytoskeleton assays after 24 hr.…”

Section: Bacteria and Cell Studiesmentioning

confidence: 99%

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Enhanced corrosion resistance and reduced cytotoxicity of the AZ91 Mg alloy by plasma nitriding and a hierarchical structure composed of ciprofloxacin‐loaded polymeric multilayers and calcium phosphate coating

Shanaghi

Mehrjou

Chu

2021

J Biomedical Materials Res

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Much effort has made to lessen the cytotoxicity and enhance the corrosion resistance of biodegradable magnesium alloys, for example, by depositing multilayered polymeric coatings containing hydroxyapatite. In this work, a hierarchical structure composed of ciprofloxacin (Cip)‐loaded on polyacrylic acid (PAA) and poly (ethyleneimine) (PEI) as biocompatible polymeric multilayers and calcium phosphate coating as the top layer is formed by the sol–gel method on the AZ91 Mg alloy with an intermediate layer formed by nitrogen plasma immersion ion implantation. The thicknesses of the multilayered coating and nitrided layer (Mg3N2) are 10 μm and 140 nm, respectively. The corrosion current density decreases by 95.6% and the corrosion potential in the polarization curve shifts to the positive direction by 23%. The passivation process which occurs at defects by deposition of corrosion products mitigates both galvanic and localized corrosion. Slight increase in the contact angle and surface free energy, enhanced corrosion resistance, and reduced cytotoxicity are observed from the multilayered structure. The better corrosion resistance enables better control of release of Cip. Biological assessment indicates that the antibacterial activity against Escherichia coli is improved by 100% after culturing for 24 hr and the cell viability and noncytotoxic behavior of the coated AZ91 are enhanced as well. The corrosion behavior and biological results suggest that the strategy of using a hierarchical structure composed of Cip‐loaded polymeric multilayers in conjunction with an intermediate plasma nitrided layer has large potential in the development of biodegradable orthopedic implants made of Mg alloys.

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Section: Discussionmentioning

confidence: 67%

Section: Methodsmentioning

confidence: 99%

Section: Bacteria and Cell Studiesmentioning

confidence: 99%

Section: Bacteria and Cell Studiesmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced corrosion resistance and reduced cytotoxicity of the AZ91 Mg alloy by plasma nitriding and a hierarchical structure composed of ciprofloxacin‐loaded polymeric multilayers and calcium phosphate coating

Shanaghi

Mehrjou

Chu

2021

J Biomedical Materials Res

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“…Mg alloy is extremely sensitive to galvanic corrosion, leading to a rapid corrosion [3][4][5]. In addition, Mg alloy also has a tendency of localized corrosion, leading to a quick failure of mechanical properties [6,7]. To protect Mg alloy against corrosion and to maintain their mechanical integrity, various kinds of anti-corrosion techniques have been developed, such as phosphating treatment, microarc oxidation and polymer coating [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic Treatment Induced Fluoride Conversion Coating without Pores for High Corrosion Resistance of Mg Alloy

Sheng¹,

Yi²,

Zhu³

et al. 2020

Coatings

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Fluoride conversion (MgF2) coating with facile preparation and good adhesion is promising to protect Mg alloy, but defects of pores in the coating lead to limited corrosion resistance. In this study, a compact and dense MgF2 coating was prepared by the combination of fluoride treatment and ultrasonic treatment. The ultrasonically treated MgF2 coating showed a compact and dense structure without pores at the frequency of 28 kHz. The chemical compositions of the coating were mainly composed of F and Mg elements. The corrosion potential of the ultrasonically treated Mg alloy shifted towards the noble direction in the electrochemical tests. The corrosion current density decreased due to the protectiveness of MgF2 coating without defects of pores or cracks. During immersion tests for 24 h, the ultrasonically treated Mg alloy exhibited the lowest H2 evolution (0.32 mL/cm2) and pH value (7.3), which confirmed the enhanced anti-corrosion ability of MgF2 coating. Hence, the ultrasonically treated fluoride coating had great potentials for their use in anti-corrosion applications of Mg alloy.

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A Biomimetic Nano‐Engineered Platform for Functional Tissue Engineering of Cartilage Superficial Zone

Dehghan-Baniani

Mehrjou

Chu

et al. 2020

Adv Healthcare Materials

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Articular cartilage has limited regeneration capacity because of its acellular and avascular nature. Although tissue engineering has been shown to be life‐saving, reforming cartilage zones required by the appropriate tissue functions are challenging. Herein, the need is addressed by designing and producing a nano‐engineered structure mimicking the superficial zone (SZ) of articular cartilage. The substrate is based on silk with good mechanical properties in conjunction with nano‐topographical and biochemical cues. Nanopillar arrays are produced on the silk surface to regulate the stem cell morphology rendering them with a flattened ellipsoidal shape that is similar to that of chondrocytes in the SZ of natural cartilage. The cell interactions are enhanced by nitrogen ion implantation and the biomolecule, kartogenin (KGN), is loaded to promote chondrogenesis of the stem cells and furthermore, a thermosensitive chitosan hydrogel is formed on the nanopatterned silk to produce rheological properties similar to those of a synovial fluid. Based on the in vitro results and mechanical properties, it is a desirable implantable smart structure mimicking the cartilage SZ with the ability of continuous drug release for cartilage regeneration.

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Nanopatterned silk-coated AZ31 magnesium alloy with enhanced antibacterial and corrosion properties

Cited by 26 publications

References 60 publications

Enhanced corrosion resistance and reduced cytotoxicity of the AZ91 Mg alloy by plasma nitriding and a hierarchical structure composed of ciprofloxacin‐loaded polymeric multilayers and calcium phosphate coating

Enhanced corrosion resistance and reduced cytotoxicity of the AZ91 Mg alloy by plasma nitriding and a hierarchical structure composed of ciprofloxacin‐loaded polymeric multilayers and calcium phosphate coating

Ultrasonic Treatment Induced Fluoride Conversion Coating without Pores for High Corrosion Resistance of Mg Alloy

A Biomimetic Nano‐Engineered Platform for Functional Tissue Engineering of Cartilage Superficial Zone

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