2019
DOI: 10.1038/s41467-019-10147-7
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Nanopore blockade sensors for ultrasensitive detection of proteins in complex biological samples

Abstract: Nanopore sensors detect individual species passing through a nanoscale pore. This experimental paradigm suffers from long analysis times at low analyte concentration and non-specific signals in complex media. These limit effectiveness of nanopore sensors for quantitative analysis. Here, we address these challenges using antibody-modified magnetic nanoparticles ((anti-PSA)-MNPs) that diffuse at zero magnetic field to capture the analyte, prostate-specific antigen (PSA). The (anti-PSA)-MNPs are magnetically driv… Show more

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Cited by 139 publications
(161 citation statements)
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“…Furthermore, some assay formats at the single molecule level have the ability to differentiate specific from nonspecific signals. This is what we achieved with the nanopore blockade sensor where nonspecific blockades could be differentiated from specific signals by using the magnet to pull the blocking magnetic nanoparticles out of the nanopore . Similarly, we also showed with single molecule SERS experiments that employed poly( N ‐isopropylacrylamide) traps to capture single molecules, and that nonspecific effects due to molecules adsorbing onto the polymer could be differentiated based on the magnitude of the single molecule SERS signal …”
Section: How Nanoparticles Solve the Challenges Faced By Single Molecsupporting
confidence: 54%
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“…Furthermore, some assay formats at the single molecule level have the ability to differentiate specific from nonspecific signals. This is what we achieved with the nanopore blockade sensor where nonspecific blockades could be differentiated from specific signals by using the magnet to pull the blocking magnetic nanoparticles out of the nanopore . Similarly, we also showed with single molecule SERS experiments that employed poly( N ‐isopropylacrylamide) traps to capture single molecules, and that nonspecific effects due to molecules adsorbing onto the polymer could be differentiated based on the magnitude of the single molecule SERS signal …”
Section: How Nanoparticles Solve the Challenges Faced By Single Molecsupporting
confidence: 54%
“…As such, this becomes a really important question as in many of the systems discussed above, the single molecule binding pair appears to have an unlimited lifetime. This is certainly the case for not only the SiMoA system11a and the nanopore blockade sensor where the nanoparticles are confined into small volumes but also for systems described above where nanoparticles simply bind to a surface 10a,19,28,29b,c. In all cases, where long lifetimes are observed however, the nanoparticles have many receptors conjugated.…”
Section: How Nanoparticles Solve the Challenges Faced By Single Molecmentioning
confidence: 93%
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