Nanopore detection of double stranded DNA using a track-etched polycarbonate membrane

Keçeci, Kaan; San, Nevim; Kaya, Dila

doi:10.1016/j.talanta.2015.06.005

Cited by 25 publications

(26 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 246 ] As solid‐state nanopores, track‐etched polymer nanopores are also used for DNA detection. [ 233,234,247 ] Kaya et al. investigated the effect of the pore geometry on DNA translocation by fabricating conical PET nanopores with different angles using methanol.…”

Section: Applicationmentioning

confidence: 99%

Track‐Etched Nanopore/Membrane: From Fundamental to Applications

2020

View full text Add to dashboard Cite

Biomimetic and bio‐inspired membranes draw great attention and generate extensive efforts to solve environmental, health, and energy issues. In this field, track‐etched membranes present the advantage to be tuned from a single pore to a large‐scale multipore membrane. On one hand, from the large‐scale membrane to a single nanopore, the research becomes more fundamental, meticulous, and quantitative to describe concrete processes inside confined spaces. This allows understanding the role of the surface phenomena and nanoscale effects. On the other hand, working from a single pore to a multipore membrane allows perfect control of the membrane properties. This ensures a promising ability for upscale from the lab findings to applications. In this review, a global insight on the track‐etched nanopore/membrane is taken through presentation of fabrication and functionalization methods, transport properties, and the related applications. By functionalization, track‐etched nanopores can be used to understand ion transport under confined spaces with high aspect ratio, to analyze single molecules, to design stimuli‐responsive membranes, and to generate energy from salinity gradient and light.

show abstract

Section: Applicationmentioning

confidence: 99%

Track‐Etched Nanopore/Membrane: From Fundamental to Applications

2020

View full text Add to dashboard Cite

show abstract

“…Conically shaped nanopores were obtained using a previously discussed asymmetric chemical etching technique [37]. Shortly, the membrane was mounted between the two halves of a conductivity cell with UV-treated side of the membrane facing the alkaline etching solution (9 M NaOH) and the other side facing the stopping solution (1 M HCOOH and 1 M KCl).…”

Section: Fabrication Of Nanoporesmentioning

confidence: 99%

Transport Characteristics of Selected Dyes Through Track-Etched Multiporous Pet Membranes

Kaya¹,

Keçeci²

2018

HJBC

Self Cite

View full text Add to dashboard Cite

Ö Z E tkin ayırma ve algılama sağlayabilecek uygulamaların elde edilebilmesi amacıyla iz-aşındırılmış poli (etilen tereftalat) (PET) membranlardan taşınım özellikleri araştırılmıştır. Simetrik ve asimetrik izaşındırma yöntemleri ile PET membranlarda silindirik ve konik nanogözenekler oluşturulmuştur. Elde edilen nanogözenekler yüklü boyar maddelerin taşınımı amacıyla kullanılmıştır. Kristal Viyole boyar maddesi için potansiyel, sıcaklık ve gözenek geometrisinin taşınıma etkileri incelenmiştir. Metil Oranj boyar maddesinin taşınımı da ayrıca incelenmiş ve PET nanogözeneklerinin duvarında bulunan negatif yüklü karboksil grupları ve uygulanan potansiyelin katyonların seçici taşınımını arttırdığı gösterilmiştir. Sıcaklık ve konik geometrinin de katyonların taşınımını arttırdığı bulunmuştur.Anahtar Kelimeler iz-aşındırılmış nanogözenek; kütle taşınımı; PET membran; kristal viyole, metil oranj. A B S T R A C TW e have investigated the transport properties of track-etched poly(ethylene terephthalate) (PET) membranes in order to pursue possible applications for effective separation and sensing purposes. We have obtained cylindrical and conical nanopores on PET membranes using symmetrical and asymmetrical track-etch methods, respectively. We have used the fabricated nanopores for the transport of charged dye molecules. Effect of applied potential, temperature and pore geometry was shown for crystal violet dye. We have also investigated the transport of methyl orange and shown negatively charged carboxylate groups on the PET nanopore walls along with applied potential enhanced the selective transport of cations. The temperature and the conical geometry were also found to promote the transport of cations.

show abstract

“…This signal is called a resistive pulse and carries information about the size, number or charge of the particles such as proteins, [5] viruses [6] and DNA [7] . Synthetic nanopores are particularly preferred in RPS since they allow control over the size and geometry of the pore and they have some desired properties such as being reproducible, stable and chemically and thermally robust [8,9] …”

Section: Introductionmentioning

confidence: 99%

Role of Nanopore Geometry in Particle Resolution by Resistive‐Pulse Sensing

et al. 2021

Self Cite

View full text Add to dashboard Cite

Nanopores as resistive pulse sensors can effectively detect single‐particles of several types including molecules without the need of labelling. Although pore geometry has a significant role in the formation of the obtained signals, it has been investigated relatively little. In the present study, we run simulations to consider the effects of pore geometry and compare conical, cigar and hourglass shaped pores at the conditions where both the resistive and conductive pulses occur in the conical pore. We examine the pulse magnitudes in relation to variations in size and charge for spherical particles. We also compare the pore geometries for when the particles pass through an off‐axis trajectory, close to the pore wall. The results show that distorting the conical shape into an hourglass or a cigar shape can significantly enhance the sensing capability of the nanopore. We show that at identical conditions, the hourglass pore only generates resistive pulses unlike the other two geometries. In the absence of the conductive pulses, the hourglass pore is robust to the variations in the particle charge and its pulse magnitudes are less affected by the particle trajectory than the cigar pore. The comparison shows that the hourglass pore may yield the best performance for size discrimination purposes. On the other hand, the conductive pulses carry valuable information and accordingly, by the aid of conductive pulses, the cigar pore can outperform the other geometries for the detection of charged particles.

show abstract

Nanopore detection of double stranded DNA using a track-etched polycarbonate membrane

Cited by 25 publications

References 60 publications

Track‐Etched Nanopore/Membrane: From Fundamental to Applications

Track‐Etched Nanopore/Membrane: From Fundamental to Applications

Transport Characteristics of Selected Dyes Through Track-Etched Multiporous Pet Membranes

Role of Nanopore Geometry in Particle Resolution by Resistive‐Pulse Sensing

Contact Info

Product

Resources

About