Nanopore long-read transcriptome data of fungal pathogen of chalkbrood disease,<i>Ascosphaera apis</i>

Du, Yasong; Chen, H; Wang, J; Zhu, Zhenggang; Zheng, Yusheng; Chen, D; Guo, Rong

doi:10.1101/2020.03.12.989863

Cited by 5 publications

(5 citation statements)

References 2 publications

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“…As shown in Table 3 in Fujian Agriculture and Forestry University. According to the previous method described by Jensen et al [2] with some minor modifications [3], mycelium sampel and spore sample were respectively preparaed and frozen in liquid nitrogen, followed by storage at -80 °C until PacBio SMRT sequencing.…”

Section: Data Descriptionmentioning

confidence: 99%

PacBio SMRT-based full-length transcriptome data ofAscosphaera apismycelium and spore

Wang

Chen

et al. 2020

Preprint

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Ascosphaera apis is an entomopathogenic fungus that exclusively infects honeybee larvae, resulting in chalkbrood disease, a widespread fungal disease damaging the beekeeping industry all over the world. In this article, purified mycelia (Aam) and spores (Aas) of A. apis pure culture under lab condition were sequenced using PacBio Sequel platform. In total, 13,302,489 and 9,911,345 subreads were yielded from Aam and Aas, respectively; 394,142 and 274,928 circular consensus sequence (CCS) reads were identified as being full-length non-chimeric (FLNC) reads, with a mean length of 2820 bp and 2602 bp, respectively. Furthermore, 174,095 and 103,845 corrected isoforms were identified, with a N50 length of 3543 bp and 3262 bp, respectively. The reported full-length transcriptome data of A. apis mycelium and spore will provide a valuable resource for improvement of genome and transcriptome annotations as well as better understanding of transcript structure such as alternative splicing and polyadenylation. Value of the data• Current dataset offers a set of high-quality full-length transcripts of A. apis.• The data can facilitate the improvement of A. apis genome and transcriptome annotations.• This dataset benefits further exploration of alternative splicing and polyadenylation of A. apis mRNAs.

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Section: Data Descriptionmentioning

confidence: 99%

PacBio SMRT-based full-length transcriptome data ofAscosphaera apismycelium and spore

Wang

Chen

et al. 2020

Preprint

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“…apis was previously isolated from a fresh chalkbrood mummy of Apis mellifera ligustica larva [1] and kept at the Honeybee Protection Laboratory of the College of Animal Sciences (College of Bee Science) in Fujian Agriculture and Forestry University. A. apis mycelia and spores were respectively purified based on the previously developed method by Jensen et al [2] with some modifications [3]. After purification, mycelium sample and spores sample were immediately frozen in liquid nitrogen and stored at -80 °C until MeRIP-seq and RNA-seq.…”

Section: Sample Collectionmentioning

confidence: 99%

MeRIP-seq and RNA-seq data of mycelium and spore ofAscosphaera apis, a widespread bee fungal pathogen

Jiang

Zhu

et al. 2020

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Ascosphaera apis is widespread fungal pathogen of honeybee larvae, causing chalkbrood, a chronic disease that weakens bee health and colony productivity. In this article, mecylia and spores of A. apis were respectively purified followed by RNA isolation, cDNA library construction, MeRIP-seq and RNA-seq. A total of 62,551,172, 41,773,158, 49,535,092 and 61,569,610 raw reads were produced from Aam_IP, Aas_IP, Aam_input and Aas_input groups, respectively. After quality control, 58,484,368, 37,381,432, 44,655,434 and 58,739,742 clean reads were obtained. Furthermore, 47,706,205, 31,356,690, 35,259,810 and 44,319,061 clean reads were mapped to the reference genome of A. apis, including 39,337,036, 26,731,957, 31,987,396 and 40,017,855 unique mapped reads, and 8,369,169, 4,624,733, 3,272,414 and 4,301,206 multiple mapped reads. Among them, 96.31%, 96.51% , 96.82% and 97.11% of clean reads were mapped to exons; 2.09%, 2.31%,1.83% and 1.81% to introns; 1.60%, 1.18%, 1.35% and 1.08% to intergenic regions.

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“…University. Based on previously described approach [2] with several minor modifications [3], fungal mycelium sample and spore sample were respectively purified and immediately frozen in liquid nitrogen followed by storage at -80 °C until WGBS.…”

Section: Data Descriptionmentioning

confidence: 99%

Whole genome bisulfite sequencing dataset of mycelium and spore of chalkbrood disease pathogenAscosphaera apis

Jiang

Chen

et al. 2020

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Chalkbrood, a widespread fungal disease of bee larvae, is caused by the fungus Ascosphaera apis. In this article, mecylia and spores of A. apis were respectively collected followed by DNA isolation, bisulfite conversion, cDNA library construction and next-generation sequencing. Using whole genome bisulfite sequencing (WGBS), 69,844,360 and 60,570,990 raw reads were yielded from Aam and Aas, and after quality control, 9,982,386,951 and 8,825,601,434 clean reads were obtained, respectively. In addition, 67,685,866 and 58,886,072 clean reads were mapped to the reference genome of A. apis, including 37,643,592 and 31,568,442 unique mapped clean reads, and 49,686 and 13,348 multiple mapped clean reads. Furthermore, after bisulfite treatment, the conversion ratio of clean reads from Aam and Aas were 99.38% and 99.51%, respectively. The WGBS data ducumented here contributes to genome-wide identification of 5mC methylation sites in A. apis and comparison of methylomes between mycelium and spore. Value of the dataThis dataset can be used for genome-wide identification of 5mC methylation sites in A. apis.The accessible data could be used to systematically compare methylomes between mycelium and spore of A. apis.Current data provides a useful resource for further study on DNA methylation-mediated mechanism underlying mycelium growth, spore germination and sexual reproduction of mycelium with the opposite sex.

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Nanopore long-read transcriptome data of fungal pathogen of chalkbrood disease,Ascosphaera apis

Cited by 5 publications

References 2 publications

PacBio SMRT-based full-length transcriptome data ofAscosphaera apismycelium and spore

PacBio SMRT-based full-length transcriptome data ofAscosphaera apismycelium and spore

MeRIP-seq and RNA-seq data of mycelium and spore ofAscosphaera apis, a widespread bee fungal pathogen

Whole genome bisulfite sequencing dataset of mycelium and spore of chalkbrood disease pathogenAscosphaera apis

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