Nanoporentechniken zur Sequenzierung und Detektion von Nukleinsäuren

Ying, Yi‐Lun; Zhang, Junji; Gao, Rui; Long, Yi‐Tao

doi:10.1002/ange.201303529

Cited by 24 publications

(4 citation statements)

References 76 publications

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“…Nanopore‐based sensors for nucleic‐acid detection and sequencing is a thriving field of research 99. Nanopores have been exploited also for single‐molecule miRNA detection, as reported by Wanunu et al., who were the first to describe a sensor for miR‐122 based on this approach 100.…”

Section: Nanotechnology‐based Strategiesmentioning

confidence: 99%

Nanotechnology‐Based Strategies for the Detection and Quantification of MicroRNA

Degliangeli

Pompa

Fiammengo

2014

Chemistry A European J

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MicroRNAs (miRNAs) are important regulators of gene expression, and many pathological conditions, including cancer, are characterized by altered miRNA expression levels. Therefore, accurate and sensitive quantification of miRNAs may result in correct disease diagnosis establishing these small noncoding RNA transcripts as valuable biomarkers. Aiming at overcoming some limitations of conventional quantification strategies, nanotechnology is currently providing numerous significant alternatives to miRNA sensing. In this review an up-to-date account of nanotechnology-based strategies for miRNA detection and quantification is given. The topics covered are: nanoparticle-based approaches in solution, sensing based on nanostructured surfaces, combined nanoparticle/surface sensing approaches, and single-molecule approaches.

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Section: Nanotechnology‐based Strategiesmentioning

confidence: 99%

Nanotechnology‐Based Strategies for the Detection and Quantification of MicroRNA

Degliangeli

Pompa

Fiammengo

2014

Chemistry A European J

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“…Viele spezifische Aspekte der Protein-und DNA-Sequenzierung sind in früheren Übersichtsartikeln dargestellt worden und werden hier deshalb nicht detailliert beschrieben. [17] 2. Sequenzierung von Biopolymeren…”

Section: Einführungunclassified

“Lesen” von Polymeren: Die Sequenzierung natürlicher und synthetischer Makromoleküle

Mutlu¹,

Lutz²

2014

Angewandte Chemie

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Die Sequenzierung von Biopolymeren wie Proteine und DNA zählt zu den bedeutendsten wissenschaftlichen Leistungen des 20. Jahrhunderts. Tatsächlich ist es mithilfe moderner chemischer Methoden der Sequenzanalyse möglich geworden, den “Code des Lebens” zu lesen und zu verstehen. Somit spielen Sequenzierungsverfahren derzeit eine wichtige Rolle bei so unterschiedlichen Anwendungen wie der Genomik, der Gentherapie, der Biotechnologie und der Datenspeicherung. Letzten Endes ist die Sequenzierung aber keine spezifisch biologische Methodik, sondern lässt sich allgemein als ein Problem der makromolekularen Chemie auffassen, definiert als die Analyse von Monomersequenzen in einem Polymer. Allerdings haben sich die Verfahren zur Untersuchung von Monomersequenzen in biologischen und synthetischen Polymeren in der Vergangenheit stark unterschieden. Mit der neuesten Entwicklung von synthetischen sequenzkontrollierten Polymeren verschwinden diese “kulturellen” Unterschiede jedoch langsam. Das Ziel dieses Kurzaufsatzes ist es, einen Überblick über die Hilfsmittel zu geben, die gegenwärtig für die Sequenzanalyse in der makromolekularen Wissenschaft zur Verfügung stehen.

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“…Many specific aspects of protein and DNA sequencing have been described in previous reviews and are therefore not described in detail in the present text. [17] Thus, the present Minireview was conceived to be a concise introduction to the important field of macromolecular sequencing.…”

Section: Introductionmentioning

confidence: 99%

Reading Polymers: Sequencing of Natural and Synthetic Macromolecules

2014

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The sequencing of biopolymers such as proteins and DNA is among the most significant scientific achievements of the 20th century. Indeed, modern chemical methods for sequence analysis allow reading and understanding the codes of life. Thus, sequencing methods currently play a major role in applications as diverse as genomics, gene therapy, biotechnology, and data storage. However, in terms of fundamental science, sequencing is not really a question of molecular biology but rather a more general topic in macromolecular chemistry. Broadly speaking, it can be defined as the analysis of comonomer sequences in copolymers. However, relatively different approaches have been used in the past to study monomer sequences in biological and manmade polymers. Yet, these "cultural" differences are slowly fading away with the recent development of synthetic sequence-controlled polymers. In this context, the aim of this Minireview is to present an overview of the tools that are currently available for sequence analysis in macromolecular science.

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Nanoporentechniken zur Sequenzierung und Detektion von Nukleinsäuren

Cited by 24 publications

References 76 publications

Nanotechnology‐Based Strategies for the Detection and Quantification of MicroRNA

Nanotechnology‐Based Strategies for the Detection and Quantification of MicroRNA

“Lesen” von Polymeren: Die Sequenzierung natürlicher und synthetischer Makromoleküle

Reading Polymers: Sequencing of Natural and Synthetic Macromolecules

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