Nanoporous gold as a suitable substrate for preparation of a new sensitive electrochemical aptasensor for detection of Salmonella typhimurium

Ranjbar, Saba; Shahrokhian, Saeed; Nurmohammadi, Fatemeh

doi:10.1016/j.snb.2017.08.160

Cited by 99 publications

(39 citation statements)

References 42 publications

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“…Comparing with other electrochemical label-free immunosensors in the literature (Table 1) we achieved to a very good LOD, even some of them accomplished lower values [11,48,49]. Specifically, Zelada-Guillén et al (2009) and Ranjbar et al (2018) works reported LODs as low as 1 cell mL −1 that was aligned to the legislated limits. Although, in Zelada-Guillén et al (2009) work a low ionic strength media was needed to perform the analysis and the method applicability to real samples was not stated.…”

Section: Immunosensor Performancesupporting

confidence: 61%

“…Although, in Zelada-Guillén et al (2009) work a low ionic strength media was needed to perform the analysis and the method applicability to real samples was not stated. Instead, Ranjbar et al (2018) presented the applicability test in a complex sample, despite the multi-step sample preparation and the high contamination levels tested. Likewise, the assumed LOD was quite far from the lower limit of the linear range, increasing the uncertainty at low contamination levels.…”

Section: Immunosensor Performancementioning

confidence: 99%

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In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

Silva

Magalhães

Barroso

et al. 2019

Talanta

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Polymeric ion selective electrodes are highly sensitive to changes in zero current ion flow and this offers a route to signal amplification in label-free potentiometric immunosensors. In this work, a label-free potentiometric immunosensor toward Salmonella typhimurium (ST) assembled in a home-made pipette-tip electrode is described. The signal-output amplification was implemented on a gold nanoparticle polymer inclusion membrane (AuNPs-PIM) which was used as sensing platform and for antibody immobilization. Additionally, a marker ion was used to detect the antibody-antigen binding event at the electrode surface. The immunosensor construction was performed in several steps: i) gold salt ions extraction in PVC membrane; ii) AuNPs formation using Na 2 EDTA as reduction agent; iii) antibody anti-Salmonella conjugation on AuNPs-PIM in pipette-tip electrodes. The potential shift observed in potentiometric measurements was derived simply from the blocking effect in the ionic flux caused by antigen-antibody conjugation, without no extra steps, mimetizing the ion-channel sensors. A detection limit of 6 cells mL −1 was attained. As proof-of-concept, recovery studies were performed in spiked commercial apple juice samples with success. Due to the simplicity of use, the appealing cost of equipment and sensor production and being able to provide a quick analytical response (less than 1 h for a complete assay, including sample preparation for analysis), this scheme represents a good prototype device for the detection of foodborne pathogens like ST or other immune-responsive bacteria.

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Section: Immunosensor Performancesupporting

confidence: 61%

Section: Immunosensor Performancementioning

confidence: 99%

In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

Silva

Magalhães

Barroso

et al. 2019

Talanta

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“…Comparing with other electrochemical immunosensors (Altintas et al, 2018;Bhardwaj et al, 2017;Burrs et al, 2016;Khan et al, 2018) we can assume similar or higher time resolution of method, such as a higher range of quantification. Lower LOD values were attained with potentiometric aptamer resorting to the SWCNTs and graphene as ion-to-electron transducers (Hernández et al, 2014;Zelada-Guillén et al 2009 and nonporous gold modified platforms (Ranjbar et al, 2018), although this is the first time that such low LOD is reached for foodborne pathogens resorting to a potentiometric paper-based strip electrode. Some colorimetric methods have been used for the range reported, although the quantification at this level of infection can't be addressed (Hossain et al, 2012;Li et al, 2017;Srisa-Art et al, 2018;Suaifan et al, 2017a, b).…”

Section: Immunosensor Performancementioning

confidence: 88%

“…Important goals have been achieved with the developed electrochemical biosensors. Recently, some potentiometric (Hernández et al, 2014;Zelada-Guillén et al 2009 and impedimetric (Ranjbar et al, 2018) label-free aptasensors such as a amperometric genosensor (Liébana et al, 2009) presented capabilities to detect a single cell in a small buffered sample volume in a few minutes. Despite this important outcoming in rapid zero tolerance electrochemical biosensing systems, the final product is still expensive due to the high cost of the electrodes and bioreceptors.…”

Section: Introductionmentioning

confidence: 99%

Development of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogen

Silva

Almeida

Magalhães

et al. 2019

Biosensors and Bioelectronics

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This work reports a new paper-based sensing platform and its application in a label-free potentiometric immunosensor for Salmonella typhimurium detection based on the blocking surface principle. A paper-based strip electrode was integrated with a filter paper pad which acted as a reservoir of the internal solution. The design offers a convenient platform for antibody immobilization and sampling, proving also that is a simple and affordable methodology to control an ionic flux through a polymer membrane. Two different immunosensing interfaces were assembled on the developed paper-strip electrode. The simplest interface relied on direct conjugation of the antibody to the polymer membrane and the second one resorted to an intermediate layer of a polyamidoamine dendrimer, with an ethylenediamine core from the fourth generation. Electrochemical impedance spectroscopy was used to assess the successive interface modification steps and the resulting analytical performance of both immunosensors was compared. For such, the potential shift derived from the blocking effect of the ionic flux caused by antigen-antibody conjugation was correlated with the logarithm of the Salmonella typhimurium concentration in the sample. In optimized conditions, a limit of detection of 5 cells mL −1 was achieved. As a proof-of-concept, the proposed method was applied to apple juice samples, demonstrating to be a suitable prototype to be used in real scenarios in useful time (< 1 h assay).

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“…Herein, we focused on impedimetric aptamer sensors. Previously reported targets include: Small molecules like progesterone [9] or Ochratoxin A (OTA) [10], proteins such as clinically relevant Vascular Endothelial Growth Factor (VEGF) [11,12] or carcinoembryonic antigen (CEA) [13], spore simulants of Bacillus anthracis [14], and various bacterial pathogens (e.g., Salmonella typhimurium [15,16], Escherichia coli (E. coli) [17][18][19][20], Staphylococcus aureus [21,22]). Infections with specific pathogenic E. coli, for instance, are the cause of multiple severe medical conditions [23].…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Flow Cells for Aptamer-Based Impedimetric Detection of E. coli Crooks Strain

Siller

Preuß

Urmann

et al. 2020

Sensors

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Electrochemical spectroscopy enables rapid, sensitive, and label-free analyte detection without the need of extensive and laborious labeling procedures and sample preparation. In addition, with the emergence of commercially available screen-printed electrodes (SPEs), a valuable, disposable alternative to costly bulk electrodes for electrochemical (bio-)sensor applications was established in recent years. However, applications with bare SPEs are limited and many applications demand additional/supporting structures or flow cells. Here, high-resolution 3D printing technology presents an ideal tool for the rapid and flexible fabrication of tailor-made, experiment-specific systems. In this work, flow cells for SPE-based electrochemical (bio-)sensor applications were designed and 3D printed. The successful implementation was demonstrated in an aptamer-based impedimetric biosensor approach for the detection of Escherichia coli (E. coli) Crooks strain as a proof of concept. Moreover, further developments towards a 3D-printed microfluidic flow cell with an integrated micromixer also illustrate the great potential of high-resolution 3D printing technology to enable homogeneous mixing of reagents or sample solutions in (bio-)sensor applications.

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Nanoporous gold as a suitable substrate for preparation of a new sensitive electrochemical aptasensor for detection of Salmonella typhimurium

Cited by 99 publications

References 42 publications

In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

Development of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogen

3D-Printed Flow Cells for Aptamer-Based Impedimetric Detection of E. coli Crooks Strain

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