Nanoreinforcement of Poly(propylene fumarate)-Based Networks with Surface Modified Alumoxane Nanoparticles for Bone Tissue Engineering

Horch, R. Adam; Shahid, Naureen; Mistry, Amit S.; Timmer, Mark D.; Mikos, Antonios G.; Barron, Andrew R.

doi:10.1021/bm049768s

Cited by 101 publications

(84 citation statements)

References 30 publications

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“…Recently, carbon nanotubes and alumoxane nanoparticles have been examined as reinforcing fillers for biodegradable polymers. [40][41][42] The effect of a filler on mechanical properties is dependent on the size, shape, and dispersion of the filler. In addition, the interaction between the filler and the organic matrix can also impact the level of reinforcement.…”

Section: Nanocomposite Constructsmentioning

confidence: 99%

“…Therefore, optimal performance is achieved when the small particles are uniformly dispersed throughout the polymer and interact strongly with the organic matrix. 41 Both nanophase additives have a tendency to aggregate, losing their nanoscale size and corresponding properties. 41,42 Therefore, surface modification is necessary to improve miscibility such that a uniform dispersion may be achieved.…”

Section: Nanocomposite Constructsmentioning

confidence: 99%

See 1 more Smart Citation

Nanobiomaterial applications in orthopedics

Christenson

Anseth

Beucken

et al. 2006

Journal Orthopaedic Research

Self Cite

327

203

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Advancements in nanobiotechnology are revolutionizing our capability to understand biological intricacies and resolve biological and medical problems by developing subtle biomimetic techniques. Nanocomposites and nanostructured materials are believed to play a pivotal role in orthopedic research since bone itself is a typical example of a nanocomposite. This article reviews current strategies using nanobiomaterials to improve current orthopedic materials and examines their applications in bone tissue engineering. Preliminary investigations support the potential of nanobiomaterials in orthopedic applications; however, significant advancements are necessary to achieve clinical use. Overall, current trends in nanobiotechnology foreshadow a bright future through the use of nanobiomaterials in the orthopedic domain. ß

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Section: Nanocomposite Constructsmentioning

confidence: 99%

Section: Nanocomposite Constructsmentioning

confidence: 99%

Nanobiomaterial applications in orthopedics

Christenson

Anseth

Beucken

et al. 2006

Journal Orthopaedic Research

Self Cite

327

203

View full text Add to dashboard Cite

show abstract

“…[34][35][36] Further, studies show that SWCNT-incorporated biodegradable polymer scaffolds are osteoconductive and allow noninvasive magnetic resonance imaging to evaluate nanotube release during the polymer degradation process and their biodistribution on release from the polymer matrix. 37,38 Our results indicate that PA stimulation of SWCNT-incorporated bone tissue engineering polymer scaffolds should assist the process of osteoinduction.…”

Section: Figmentioning

confidence: 99%

A Novel Nanoparticle-Enhanced Photoacoustic Stimulus for Bone Tissue Engineering

Sitharaman

Avti²,

Schaefer³

et al. 2011

Tissue Engineering Part A

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In this study, we introduce a novel nanoparticle-enhanced biophysical stimulus based on the photoacoustic (PA) effect. We demonstrate that the PA effect differentiates bone marrow-derived marrow stromal cells (MSCs) grown on poly(lactic-co-glycolic acid) (PLGA) polymer films toward osteoblasts. We further show that the osteodifferentiation of the MSCs due to PA stimulation is significantly enhanced by the presence of single-walled carbon nanotubes (SWCNTs) in the polymer. MSCs, without the osteogenic culture supplements (0.01 M bglycerophosphate, 50 mg/L ascorbic acid, 10 -8 M dexamethasone), were seeded onto plain glass slides, glass slides coated with PLGA, or glass slides coated with SWCNT-PLGA films and photoacoustically stimulated by a 527 nm Nd:YLF pulse laser, with a 200 ns pulse duration, and 10 Hz pulse frequency for 10 min a day for 15 consecutive days. The study had four control groups; three baseline controls similar to the three experimental groups but without PA stimulation, and one positive control where MSCs were grown on glass slides without PA stimulation but with osteogenic culture supplements. The osteogenic differentiation of all the groups was evaluated using quantitative assays (alkaline phosphatase, calcium, osteopontin) and qualitative staining (alizarin red). After 15 days, the PA stimulated groups showed up to a 350% increase in calcium content when compared with the non-PA stimulated positive control. Further, within the PA stimulated group, the PLGA-SWCNT group had 130% higher calcium values than the PLGA film without SWCNTs. These results were further corroborated by the analysis of osteopontin secretion, alkaline phosphatase expression, and qualitative alizarin red staining of extracellular matrix calcification. The results indicate that PA stimulation holds promise for bone tissue engineering and that the nanomaterials which enhance the PA effect should allow the development of biophysical rather than biochemical strategies to induce osteoinductive properties into tissue engineering scaffolds.

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“…High porosity is a major factor that is desired to increase the specific surface area for cell attachment and tissue in-growth in scaffolds [10]. The pore size must be large enough to allow accommodation of cells so that interconnected pores may facilitate uniform distribution of cells, diffusion of oxygen and nutrient, and waste exchange by cells deep within the construct [11]- [14].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Tissue Engineering Scaffold from Hydroxyapatite/Alginate Composite

Nazarpak¹,

Pourasgari²

2014

IJBBB

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Abstract-Scaffolds for bone tissue engineering must meet the functional requirements, porosity, biocompatibility, and biodegradability. In this study, hydroxyapatite was prepared by wet chemical method and incorporated into the alginate gel solution to improve both the mechanical and cell-attachment properties of the scaffolds. Next, composite scaffolds were fabricated from hydroxyapatite/alginate with different weight ratios by freeze drying method and then some of them coated with triblock copolymer and compared with others. Microstructure observation with SEM suggests the formation of about 50 micrometer size porous structure and interconnected porosity. Then, human mesenchymal stem cells were cultured on the composite scaffolds. Cells adhesion to the scaffolds was observed after three days by DAPI fluorescence microscopy in which more cells adhesion to the coated scaffolds and cells diffusion into the pores are visible. Also, cell adhesion within the structure was observed by SEM in which showed cell attachment was well in depth which confirms DAPI results. These results suggest that the triblock-coated HA/Alg porous scaffolds could provide enhanced cell adhesion and proliferation which may be a promising approach for bone tissue-engineering applications.

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Nanoreinforcement of Poly(propylene fumarate)-Based Networks with Surface Modified Alumoxane Nanoparticles for Bone Tissue Engineering

Cited by 101 publications

References 30 publications

Nanobiomaterial applications in orthopedics

Nanobiomaterial applications in orthopedics

A Novel Nanoparticle-Enhanced Photoacoustic Stimulus for Bone Tissue Engineering

Fabrication of Tissue Engineering Scaffold from Hydroxyapatite/Alginate Composite

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