Abstract:A cysteine-terminated C(RGD)(4) peptide film was fabricated on a gold electrode for improving the attachment of cells. The electrochemical signals of cyclic voltammogram from cells on a C(RGD)(4) deposited electrode was enhanced from 0.27 to 0.49 μA compared to a bare electrode. The developed cell-based sensor determined the effect of bisphenol-A (BPA) and dichlorodiphenyltrichloroethane (DTT) on the viability of HEK-293 cells by detecting decrease of reduction peaks (1.12-0.15 μA for BPA and 0.81-0.29 μA for … Show more
“…In which sulfhydryl groups of cysteine terminated RGD molecules were covalently bind to the thiolated Au surface and thus allowing the self-assembling of the molecules in two dimensionally over a gold surface [ 37 ]. Such RGD peptide modified Au surface is extra useful for establishing mammalian cells for electrochemical measurements because gold conducts electricity and makes for excellent electrical contacts [ 3 , 4 ]. Therefore, several researches have been conducted with mutagenically modified RGD tripeptides with cysteine residue (an amino acid that contains a thiol group) [ 33 ].…”
Section: Establishment Of Biomaterials On the Metal Electrode Surfacementioning
confidence: 99%
“…RGD molecules were immobilized on the Au surface either as a homogenous thin film or as a definite nanoscale pattern with such engineered molecules using self-assembly or modified self-assembly methods as shown in Fig. 6 [ 3 , 4 , 10 , 11 ]. Fig.…”
Section: Establishment Of Biomaterials On the Metal Electrode Surfacementioning
confidence: 99%
“…Formation of RGD peptide thin film on the Au surface is relatively simple and can be performed using self-assembly method as discusses in the earlier [ 3 , 4 ]. But, their defined nanoscale pattern on Au surface requires a relatively complex modified self-assembly method [ 10 , 11 ].…”
Section: Establishment Of Biomaterials On the Metal Electrode Surfacementioning
confidence: 99%
“…The nano-rod like arrangement proves better cell immolation ability than their nano dot like arrangement. These materials were proved successful for establishment of rat pheochromocytoma cell (PC-12), Human neuroblastoma cells (SH-SY5Y), Human epithelial carcinoma cells (HeLa), and Human embryonic kidney cells (HEK-293T) on the metal electrode surfaces [ 3 , 4 , 10 , 11 ].…”
Section: Role Of Biomaterials On Cell Adhesion On the Metal Electrodementioning
confidence: 99%
“…Usually adhesion molecules, proteins and peptides are used for insuring the proper cell electrode interaction of a cell based chip. Several studies have been undertaken to search for a suitable material to maintain proper cell-electrode interaction for increasing cell adhesion as well as for enhancing electron transfer efficiencies [ 2 , 3 , 10 , 28 ]. Our previous study reported that large proteins or peptides modified surfaces form a mechanical barrier at the cell electrode interface that impaired electron exchange [ 10 , 28 ].…”
Section: Role Of Biomaterials On Electrochemical Measurementsmentioning
Biomaterials having cell adhesion ability are considered to be integral part of a cell chip. A number of researches have been carried out to search for a suitable material for effective immobilization of cell on substrate. Engineered ECM materials or their components like collagen, Poly-l-Lysine (PLL), Arg-Gly-Asp (RGD) peptide have been extensively used for mammalian cell adhesion and proliferation with the aim of tissue regeneration or cell based sensing application. This review focuses on the various approaches for two- and three-dimensionally patterned nanostructures of a short peptide i.e. RGD peptide on chip surfaces together with their effects on cell behaviors and electrochemical measurements. Most of the study concluded with positive remarks on the well-oriented engineered RGD peptide over their homogenous thin film. The engineered RGD peptide not only influences cell adhesion, spreading and proliferation but also their periodic nano-arrays directly influence electrochemical measurements of the chips. The electrochemical signals found to be enhanced when RGD peptides were used in well-defined two-dimensional nano-arrays. The topographic alteration of three-dimensional structure of engineered RGD peptide was reported to be suitably contacted with the integrin receptors of cellular membrane which results indicated the enhanced cell-electrode adhesion and efficient electron exchange phenomenon. This enhanced electrochemical signal increases the sensitivity of the chip against the target analytes. Therefore, development of engineered cellular recognizable peptides and its 3D topological design for fabrication of cell chip will provide the synergetic effect on bio-affinity, sensitivity and accuracy for the in situ real-time monitoring of analytes.
“…In which sulfhydryl groups of cysteine terminated RGD molecules were covalently bind to the thiolated Au surface and thus allowing the self-assembling of the molecules in two dimensionally over a gold surface [ 37 ]. Such RGD peptide modified Au surface is extra useful for establishing mammalian cells for electrochemical measurements because gold conducts electricity and makes for excellent electrical contacts [ 3 , 4 ]. Therefore, several researches have been conducted with mutagenically modified RGD tripeptides with cysteine residue (an amino acid that contains a thiol group) [ 33 ].…”
Section: Establishment Of Biomaterials On the Metal Electrode Surfacementioning
confidence: 99%
“…RGD molecules were immobilized on the Au surface either as a homogenous thin film or as a definite nanoscale pattern with such engineered molecules using self-assembly or modified self-assembly methods as shown in Fig. 6 [ 3 , 4 , 10 , 11 ]. Fig.…”
Section: Establishment Of Biomaterials On the Metal Electrode Surfacementioning
confidence: 99%
“…Formation of RGD peptide thin film on the Au surface is relatively simple and can be performed using self-assembly method as discusses in the earlier [ 3 , 4 ]. But, their defined nanoscale pattern on Au surface requires a relatively complex modified self-assembly method [ 10 , 11 ].…”
Section: Establishment Of Biomaterials On the Metal Electrode Surfacementioning
confidence: 99%
“…The nano-rod like arrangement proves better cell immolation ability than their nano dot like arrangement. These materials were proved successful for establishment of rat pheochromocytoma cell (PC-12), Human neuroblastoma cells (SH-SY5Y), Human epithelial carcinoma cells (HeLa), and Human embryonic kidney cells (HEK-293T) on the metal electrode surfaces [ 3 , 4 , 10 , 11 ].…”
Section: Role Of Biomaterials On Cell Adhesion On the Metal Electrodementioning
confidence: 99%
“…Usually adhesion molecules, proteins and peptides are used for insuring the proper cell electrode interaction of a cell based chip. Several studies have been undertaken to search for a suitable material to maintain proper cell-electrode interaction for increasing cell adhesion as well as for enhancing electron transfer efficiencies [ 2 , 3 , 10 , 28 ]. Our previous study reported that large proteins or peptides modified surfaces form a mechanical barrier at the cell electrode interface that impaired electron exchange [ 10 , 28 ].…”
Section: Role Of Biomaterials On Electrochemical Measurementsmentioning
Biomaterials having cell adhesion ability are considered to be integral part of a cell chip. A number of researches have been carried out to search for a suitable material for effective immobilization of cell on substrate. Engineered ECM materials or their components like collagen, Poly-l-Lysine (PLL), Arg-Gly-Asp (RGD) peptide have been extensively used for mammalian cell adhesion and proliferation with the aim of tissue regeneration or cell based sensing application. This review focuses on the various approaches for two- and three-dimensionally patterned nanostructures of a short peptide i.e. RGD peptide on chip surfaces together with their effects on cell behaviors and electrochemical measurements. Most of the study concluded with positive remarks on the well-oriented engineered RGD peptide over their homogenous thin film. The engineered RGD peptide not only influences cell adhesion, spreading and proliferation but also their periodic nano-arrays directly influence electrochemical measurements of the chips. The electrochemical signals found to be enhanced when RGD peptides were used in well-defined two-dimensional nano-arrays. The topographic alteration of three-dimensional structure of engineered RGD peptide was reported to be suitably contacted with the integrin receptors of cellular membrane which results indicated the enhanced cell-electrode adhesion and efficient electron exchange phenomenon. This enhanced electrochemical signal increases the sensitivity of the chip against the target analytes. Therefore, development of engineered cellular recognizable peptides and its 3D topological design for fabrication of cell chip will provide the synergetic effect on bio-affinity, sensitivity and accuracy for the in situ real-time monitoring of analytes.
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