Nanoscale features influence epithelial cell morphology and cytokine production

Andersson, Ann‐Sofie; Bäckhed, Fredrik; Euler, Anne von; Richter‐Dahlfors, Agneta; Sutherland, Duncan S.; Kasemo, Bengt Herbert

doi:10.1016/s0142-9612(03)00208-4

Cited by 329 publications

(243 citation statements)

References 42 publications

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“…Functional changes corresponding to changes in cell morphology can range from apoptosis to proliferation, differentiation, contractility, rate of cell migration, and gene expression. [24][25][26][27]. The cellular response and sensitivity towards growth factors and mitogens may also be altered.…”

Section: Discussionmentioning

confidence: 99%

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

et al. 2008

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Peripheral nerve regeneration can be enhanced by the stimulation of formation of bands of Büngner prior to implantation. Aligned electrospun poly(ε-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to human Schwann cells. After 7 days of culture, cell cytoskeleton and nuclei were observed to align and elongate along the fiber axes, emulating the structure of bands of Büngner. Microarray analysis revealed a general down-regulation in expression of neurotrophin and neurotrophic receptors in aligned cells as compared to cells seeded on twodimensional PCL film. Real-time-PCR analyses confirmed the up-regulation of early myelination marker, MAG, and the down-regulation of NCAM-1, a marker of immature Schwann cells. Similar gene expression changes were also observed on cells cultured on randomly-oriented PCL electrospun fibers. However, up-regulation of the myelin-specific gene, P0, was observed only on aligned electrospun fibers, suggesting the propensity of aligned fibers in promoting Schwann cell maturation.

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Section: Discussionmentioning

confidence: 99%

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

et al. 2008

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“…The elongation (E) parameter describes the extent to which the equimomental eclipse is lengthened or stretched out [8]. It was calculated as the ratio of the long axis over the short axis minus one.…”

Section: Elongation and Alignment Characterizationmentioning

confidence: 99%

“…Cells in their natural environment interact with extracellular matrix (ECM) components in the nanometer scale [6]. Recent findings underscore the phenomenon that mammalian cells do respond to nanoscale features on a synthetic surface [7][8][9][10][11][12]. Nanoscaled topography of synthetic materials has been receiving increasing attention because of its resemblance to in vivo surroundings.…”

Section: Introductionmentioning

confidence: 99%

Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage

Yim

Pang

Leong

2007

Experimental Cell Research

705

677

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Human mesenchymal stem cells (hMSCs) have been shown to trans-differentiate into neuronal-like cells by culture in neuronal induction media, although the mechanism is not well understood. Topography can also influence cellular responses including enhanced differentiation of progenitor cells. As extracellular matrix (ECM) in vivo comprises topography in the nanoscale, we hypothesize that nanotopography could influence stem cell differentiation into specific non-default pathways, such as transdifferentiation of hMSC. Differentiation and proliferation of hMSCs were studied on nano-gratings of 350nm width. Cytoskeleton and nuclei of hMSCs were aligned and elongated along the nano-gratings. Gene profiling and immunostaining showed significant up-regulation of neuronal markers such as microtubule-associated protein 2 (MAP2) compared to unpatterned and micropatterned controls. The combination of nanotopography and biochemical cues such as retinoic acid further enhanced the upregulation of neuronal marker expressions, but nanotopography showed a stronger effect compared to retinoic acid alone on unpatterned surface. This study demonstrated the significance of nanotopography in directing differentiation of adult stem cells.

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“…Response of cells to topographical cues and the concept of contact guidance have been known for decades [8,9]. Various topographical features such as grooves, ridges, stops, pores, wells and nodes in micro-or nanoscale [10][11][12] have been presented to a wide variety of cells: fibroblasts [13][14][15][16][17], BHK cells [18], neuronal cells [19], macrophages [20,21], epithelial cells [22], endothelial cells, and smooth muscle cells (SMC) [23][24][25][26]. Topography can influence cellular responses from initial attachment and migration to differentiation and production of new tissue [10,12,27].…”

Section: Introductionmentioning

confidence: 99%

“…Topography can influence cellular responses from initial attachment and migration to differentiation and production of new tissue [10,12,27]. While the great majority of these cell-substrate interaction studies have been conducted on features in the micron range, recent findings underscore the phenomenon that mammalian cells do respond to nanoscale features on a synthetic surface [13,14,[22][23][24]26]. Nanoscaled topography has been receiving increasing attention because of its resemblance to in vivo surroundings.…”

Section: Introductionmentioning

confidence: 99%

Nanopattern-induced changes in morphology and motility of smooth muscle cells

Yim¹,

et al. 2005

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Cells are known to be surrounded by nanoscale topography in their natural extracellular environment. The cell behavior, including morphology, proliferation, and motility of bovine pulmonary artery smooth muscle cells (SMC) were studied on poly(methyl methacrylate) (PMMA) and poly (dimethylsiloxane) (PDMS) surfaces comprising nanopatterned gratings with 350 nm linewidth, 700 nm pitch, and 350 nm depth. More than 90% of the cells aligned to the gratings, and were significantly elongated compared to the SMC cultured on non-patterned surfaces. The nuclei were also elongated and aligned. Proliferation of the cells was significantly reduced on the nanopatterned surfaces. The polarization of microtubule organizing centers (MTOC), which are associated with cell migration, of SMC cultured on nanopatterned surfaces showed a preference towards the axis of cell alignment in an in vitro wound healing assay. In contrast, the MTOC of SMC on non-patterned surfaces preferentially polarized towards the wound edge. It is proposed that this nanoimprinting technology will provide a valuable platform for studies in cell-substrate interactions and for development of medical devices with nanoscale features.

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Nanoscale features influence epithelial cell morphology and cytokine production

Cited by 329 publications

References 42 publications

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage

Nanopattern-induced changes in morphology and motility of smooth muscle cells

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