Nanoscale Swelling Heterogeneities in Type I Collagen Fibrils

Spitzner, Eike-Christian; Röper, Stephanie; Zerson, Mario; Bernstein, Anke; Magerle, R.

doi:10.1021/nn503637q

Cited by 39 publications

(70 citation statements)

References 54 publications

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“…1 is consistent with previous studies, especially the regular D-band periodicity, which is the typical characteristics of collagen fibril. The length of the D-band periodicity is also in agreement with previous studies on the type I collagen fibril1720.…”

Section: Resultssupporting

confidence: 91%

“…The height and cantilever deflection signals were simultaneously collected during the entire indentation for elastic modulus measurement. In previous studies, it was reported that the elastic modulus of collagen fibril showed a remarkable dependence on the content of water17. Therefore, AFM measurements were carried out at a fixed humidity of 20% under RT.…”

Section: Methodsmentioning

confidence: 99%

“…Atomic force microscopy (AFM) based nanoindentation has been well established as a powerful technique for the investigation of the mechanics at the nanoscale level916171819. To have a better understanding of the effect of abnormal calcium homeostasis on soft tissues’ mechanics, we attempted to employ AFM-based nanoindentation to study the mechanical behavior of type II collagen fibril extracted from AC.…”

mentioning

confidence: 99%

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Unraveling the role of Calcium ions in the mechanical properties of individual collagen fibrils

Pang

Tang

2017

Sci Rep

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Collagen, the dominating material in the extracellular matrix, provides the strength, elasticity and mechanical stability to the organisms. The mechanical property of collagen is mainly dominated by its surrounding environments. However, the variation and origin of the mechanics of collagen fibril under different concentrations of calcium ions (χCa) remains unknown. By using the atomic force microscopy based nanoindentation, the mechanics and structure of individual type II collagen fibril were first investigated under different χCa in this study. The results demonstrate that both of the mechanical and structural properties of the collagen fibril show a prominent dependence on χCa. The mechanism of χCa-dependence of the collagen fibril was attributed to the chelation between collagen molecules and the calcium ions. Given the role of calcium in the pathology of osteoarthritis, the current study may cast new light on the understanding of osteoarthritis and other soft tissue hardening related diseases in the future.

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Section: Resultssupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Unraveling the role of Calcium ions in the mechanical properties of individual collagen fibrils

Pang

Tang

2017

Sci Rep

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“…These techniques are currently used for mechanical characterization in cell biology (living cells) and structural biology, including testing of cartilage (Heu et al, 2012), bones (Spitzner et al, 2015), soft tissues (Burgert and Keplinger, 2013), and wood (Farahi et al, 2017). Similar to nanoindentation, the main principle of force measurements is to calculate hardness and elastic modulus from a load-displacement curve recorded during a local indentation (Figures 3B,D-F).…”

Section: Quantitative Force-volume Mapping (Qfm): Mechanical Propertiesmentioning

confidence: 99%

Nanometrology of Biomass for Bioenergy: The Role of Atomic Force Microscopy and Spectroscopy in Plant Cell Characterization

et al. 2018

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Ethanol production using extracted cellulose from plant cell walls (PCW) is a very promising approach to biofuel production. However, efficient throughput has been hindered by the phenomenon of recalcitrance, leading to high costs for the lignocellulosic conversion. To overcome recalcitrance, it is necessary to understand the chemical and structural properties of the plant biological materials, which have evolved to generate the strong and cohesive features observed in plants. Therefore, tools and methods that allow the investigation of how the different molecular components of PCW are organized and distributed and how this impacts the mechanical properties of the plants are needed but challenging due to the molecular and morphological complexity of PCW. Atomic force microscopy (AFM), capitalizing on the interfacial nanomechanical forces, encompasses a suite of measurement modalities for nondestructive material characterization. Here, we present a review focused on the utilization of AFM for imaging and determination of physical properties of plant-based specimens. The presented review encompasses the AFM derived techniques for topography imaging (AM-AFM), mechanical properties (QFM), and surface/subsurface (MSAFM, HPFM) chemical composition imaging. In particular, the motivation and utility of force microscopy of plant cell walls from the early fundamental investigations to achieve a better understanding of the cell wall architecture, to the recent studies for the sake of advancing the biofuel research are discussed. An example of delignification protocol is described and the changes in morphology, chemical composition and mechanical properties and their correlation at the nanometer scale along the process are illustrated.

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“…At the fibrillar level, direct mechanical measurements have only recently become possible by atomic force microscopy (AFM) and microelectromechanical systems (MEMS). The mechanical properties of single collagen fibrils have been measured using AFM-based tensile [7][8][9][10], nanoindentation [11][12][13][14][15][16][17][18][19], and bending [20][21][22] tests, and MEMS-based tensile [23][24][25] tests.…”

Section: Introductionmentioning

confidence: 99%

Tensile Strength of Single Collagen Fibrils Isolated from Tendons

Yamamoto¹

2017

EJB

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Abstract:Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 µm, respectively.

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Nanoscale Swelling Heterogeneities in Type I Collagen Fibrils

Cited by 39 publications

References 54 publications

Unraveling the role of Calcium ions in the mechanical properties of individual collagen fibrils

Unraveling the role of Calcium ions in the mechanical properties of individual collagen fibrils

Nanometrology of Biomass for Bioenergy: The Role of Atomic Force Microscopy and Spectroscopy in Plant Cell Characterization

Tensile Strength of Single Collagen Fibrils Isolated from Tendons

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