Nanoselenium prevents eimeriosis-induced inflammation and regulates mucin gene expression in mice jejunum

Abstract

BackgroundAlthough elemental selenium has been found to be effective against Eimeria, no study has yet investigated the effects of selenium nanoparticles (SeNPs) on the Eimeria parasite. The aim of this study, therefore, was to evaluate the ameliorative effect of SeNPs compared with elemental selenium on mice jejunum infected with sporulated oocysts of Eimeria papillata.MethodsThe mice were divided into 4 groups, with the first being the non-infected, control group, and the second, third, and fourth groups being orally inoculated with 1,000 sporulated oocysts of E. papillata. The third and fourth groups also received, respectively, an oral dose of 0.1 mg/kg sodium selenite and 0.5 mg/kg SeNPs daily for 5 consecutive days.ResultsThe infection induced severe histopathological jejunal damage, reflected in the form of destroyed jejunal mucosa, increased jejunal oxidative damage, a reduction in the number of jejunal goblet cells, and increased production of pro-inflammatory cytokines, quantified by real-time polymerase chain reaction. Treatment of mice with SeNPs significantly decreased the oocyst output in the feces by ~80%. Furthermore, the number of parasitic stages counted in stained jejunal paraffin sections was significantly decreased after the mice were treated with SeNPs. In addition, the number of goblet cells increased from 42.6±7.3 to 95.3±8.5 cells/10 villus-crypt units after treatment. By day 5 post-infection with E. papillata, SeNPs could be seen to have significantly increased the activity of glutathione peroxidase from 263±10 to 402.4±9 mU/mL. Finally, SeNPs were able to regulate the gene expression of mucin 2, interleukin 1β, interleukin 6, interferon-γ, and tumor necrosis factor α in the jejunum of mice infected with E. papillata.ConclusionThe results collectively showed that SeNPs are more effective than sodium selenite with regard to their anti-coccidial, anti-oxidant, and anti-inflammatory role against eimeriosis induced in the jejunum of mice.

Selenium and nano-selenium ameliorations in two breeds of broiler chickens exposed to heat stress

“…However, the Ross broilers exhibited greater adverse effects than Arbor broilers that were supplied with the same dose of traditional commercial Se. These results were inconsistent with those of Alkhudhayri et al (2018), who reported significant and improved histological outcomes from NS supplementation in the face of environmental challenges and infection. Mousa and Ali (2018) studied the influence of nano-boron on the liver of African ostrich chicks after E. coli infection and observed the ability of nano-particles to reduce the stress factors with increased resistance.…”

Chitosan‐stabilized selenium nanoparticles attenuate acrylamide‐induced brain injury in rats

“…Nanotechnology is an emerging and rapidly growing field that has raised high expectations for a variety of applications in the food and food‐related industries, including agriculture, food processing, food packaging, and dietary supplements (He & Hwang, 2016). Recent studies have listed the various benefits of nanoparticles in the food field, which are antimicrobials, increased bioavailability, and antioxidants, as well as improving some of the rheological and phenotypic properties of foods such as color and consistency (Alkhudhayri, Dkhil, & Al‐Quraishy, 2018; Dkhil et al., 2020).…”

Food for thought: nano-selenium in poultry nutrition and health

“…Indeed, nano-Se was shown to have antimicrobial activities against Trichophyton rubrum (Yip et al ., 2014), Candida albicans (Kheradmand et al ., 2014), Pseudomonas aeruginosa, Proteus mirabilis (Shakibaie et al ., 2015), Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli O157:H7, Salmonella Typhimurium, S. Enteritidis (Khiralla and El-Deeb, 2015), S. aureus (Nguyen et al ., 2017), and Toxoplasma gondii (Keyhani et al ., 2020). Interestingly, nano-Se is more effective than sodium selenite with regard to its anti-coccidial, anti-oxidant, and anti-inflammatory role against coccidiosis induced in the jejunum of mice (Alkhudhayri et al ., 2018). Nano-Se was also shown to restrict the growth of nanobacteria (Sardarabadi et al ., 2019).…”