Nanosilk Increases the Strength of Diabetic Skin and Delivers CNP-miR146a to Improve Wound Healing

Niemiec, Stephen; Louiselle, Amanda; Hilton, Sarah A.; Dewberry, Lindel C.; Zhang, Liping; Azeltine, Mark; Xu, Jie; Singh, Sushant; Sakthivel, T.; Seal, Sudipta; Liechty, Kenneth W.; Zgheib, Carlos

doi:10.3389/fimmu.2020.590285

Cited by 44 publications

(33 citation statements)

References 39 publications

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“…There is no doubt that the specific mechanism of HUC-Exos on endothelial cells in our study needs to be further studied. According to recent reports, the proliferation and tubulogenesis of vascular endothelial cells transfected with miR-20b-5p from peripheral blood exosomes in diabetic patients were significantly reduced, and the apoptosis rate was significantly increased (Niemiec et al, 2020). In addition, exosome-derived miR-146a significantly decreased phosphorylated IκB-α and NF-κB, thereby regulating the function of vascular endothelial cells (Xiong et al, 2020).…”

Section: Discussionmentioning

confidence: 97%

Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Accelerate Diabetic Wound Healing via Ameliorating Oxidative Stress and Promoting Angiogenesis

Yan

Lin

et al. 2022

Front. Bioeng. Biotechnol.

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Diabetic wounds remain a great challenge for clinicians due to the multiple bacterial infections and oxidative damage. Exosomes, as an appealing nanodrug delivery system, have been widely applied in the treatment of diabetic wounds. Endovascular cells are important component cells of the vascular wall. Herein, we investigated the effects of HUCMSCs and HUC-Exos (exosomes secreted by HUCMSCs) on diabetic wound healing. In this study, HUVECs were coincubated with HUCMSCs, and HUC-Exos were utilized for in vitro and in vivo experiments to verify their roles in the regulation of diabetic wound healing. Our results demonstrated that HUCMSCs have the ability to regulate oxidative stress injuries of endothelial cells through exosomes and accelerate diabetic cutaneous wound healing in vitro. The present study suggests that HUC-Exos accelerate diabetic cutaneous wound healing, providing a promising therapeutic strategy for chronic diabetic wound repair.

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Section: Discussionmentioning

confidence: 97%

Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Accelerate Diabetic Wound Healing via Ameliorating Oxidative Stress and Promoting Angiogenesis

Yan

Lin

et al. 2022

Front. Bioeng. Biotechnol.

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“…Little to no immunogenicity keeps inflammation low, reducing the risk of adverse reactions [ 7 ]. Additionally, it can work synergistically when used in conjunction with other biomaterials such as chitosan or microRNA-conjugated cerium oxide nanoparticles (CNP) [ 14 ].…”

Section: Physicochemical Properties Of Silk Fibroinmentioning

confidence: 99%

“…Nanosilk is an SF-based solution which our group applied to human diabetic skin samples to improve its biochemical strength [ 14 , 70 ]. Skin samples were treated with a topical application of either 7% nanosilk or a control solution.…”

Section: Sf Solutionmentioning

confidence: 99%

Silk Fibroin-Based Therapeutics for Impaired Wound Healing

et al. 2022

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Impaired wound healing can lead to local hypoxia or tissue necrosis and ultimately result in amputation or even death. Various factors can influence the wound healing environment, including bacterial or fungal infections, different disease states, desiccation, edema, and even systemic viral infections such as COVID-19. Silk fibroin, the fibrous structural-protein component in silk, has emerged as a promising treatment for these impaired processes by promoting functional tissue regeneration. Silk fibroin’s dynamic properties allow for customizable nanoarchitectures, which can be tailored for effectively treating several wound healing impairments. Different forms of silk fibroin include nanoparticles, biosensors, tissue scaffolds, wound dressings, and novel drug-delivery systems. Silk fibroin can be combined with other biomaterials, such as chitosan or microRNA-bound cerium oxide nanoparticles (CNP), to have a synergistic effect on improving impaired wound healing. This review focuses on the different applications of silk-fibroin-based nanotechnology in improving the wound healing process; here we discuss silk fibroin as a tissue scaffold, topical solution, biosensor, and nanoparticle.

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“…CNP-miR146a improved wound healing in a murine and porcine diabetic wound model ( Zgheib et al, 2019 ). CNP-miR146a combined with an efficient delivery system, such as nanosilk ( Niemiec et al, 2020 ) or self-healable zwitterionic cryogels ( Sener et al, 2020 ), demonstrated efficacy in accelerating diabetic wound healing.…”

Section: Antioxidant Therapy In Diabetic Wound Healingmentioning

confidence: 99%

“…Nanosilk, characterized by a high strength-to-density ratio and an ability to exhibit strain hardening, shows significant application in diabetic wound healing. Niemiec et al (2020) prepared nanosilk-based materials on silk fibroin, a biocompatible polymer that can be fabricated into a nanostructure. The Nanosilk preparation showed effective delivery of CNP-miR146a to the wound bed, which downregulated pro-inflammatory signaling and promoted pro-fibrotic processes, ultimately accelerating diabetic wound healing.…”

Section: Antioxidant Therapy In Diabetic Wound Healingmentioning

confidence: 99%

Antioxidant Therapy and Antioxidant-Related Bionanomaterials in Diabetic Wound Healing

Zhang

Chen

Xiong

et al. 2021

Front. Bioeng. Biotechnol.

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Ulcers are a lower-extremity complication of diabetes with high recurrence rates. Oxidative stress has been identified as a key factor in impaired diabetic wound healing. Hyperglycemia induces an accumulation of intracellular reactive oxygen species (ROS) and advanced glycation end products, activation of intracellular metabolic pathways, such as the polyol pathway, and PKC signaling leading to suppression of antioxidant enzymes and compounds. Excessive and uncontrolled oxidative stress impairs the function of cells involved in the wound healing process, resulting in chronic non-healing wounds. Given the central role of oxidative stress in the pathology of diabetic ulcers, we performed a comprehensive review on the mechanism of oxidative stress in diabetic wound healing, focusing on the progress of antioxidant therapeutics. We summarize the antioxidant therapies proposed in the past 5 years for use in diabetic wound healing, including Nrf2- and NFκB-pathway-related antioxidant therapy, vitamins, enzymes, hormones, medicinal plants, and biological materials.

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Nanosilk Increases the Strength of Diabetic Skin and Delivers CNP-miR146a to Improve Wound Healing

Abstract: CNP-miR146a to improve diabetic wound healing through inhibition of pro-inflammatory gene signaling and promotion of pro-fibrotic processes.

Cited by 44 publications

References 39 publications

Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Accelerate Diabetic Wound Healing via Ameliorating Oxidative Stress and Promoting Angiogenesis

Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Accelerate Diabetic Wound Healing via Ameliorating Oxidative Stress and Promoting Angiogenesis

Silk Fibroin-Based Therapeutics for Impaired Wound Healing

Antioxidant Therapy and Antioxidant-Related Bionanomaterials in Diabetic Wound Healing

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