Nanostructure of the aqueous form of lung surfactant of different species visualized by cryo‐transmission electron microscopy

Waisman, Dan; Danino, Dganit; Weintraub, Zalman; Schmidt, Judith; Talmon, Yeshayahu

doi:10.1111/j.1475-097x.2007.00763.x

Cited by 20 publications

(29 citation statements)

References 24 publications

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“…including those internalized in larger ones. More generally, cryo-TEM Curosurf® vesicles are similar to those reported in the literature [23,26,45] and comparable to those of endogenous surfactants [46]. Nanoparticle tracking analysis (NTA) performed on a 10 -3 g L -1 dispersion revealed a vesicle number distribution peaked around 150 nm, in fair agreement with the cryo-TEM results (S6).…”

Section: Iii1 -Curosurf® Structuresupporting

confidence: 86%

On the rheology of pulmonary surfactant: Effects of concentration and consequences for the surfactant replacement therapy

Thai

Mousseau

Oikonomou

et al. 2019

Colloids and Surfaces B: Biointerfaces

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The role of pulmonary surfactant is to reduce the surface tension in the lungs and to facilitate breathing. Surfactant replacement therapy (SRT) aims at bringing a substitute by instillation into the airways, a technique that has proven to be efficient and lifesaving for preterm infants. Adapting this therapy to adults requires to scale the administered dose to the patient body weight and to increase the lipid concentration, whilst maintaining its surface and flow properties similar. Here, we exploit a magnetic wire-based microrheology technique to measure the viscosity of the exogenous pulmonary surfactant Curosurf® in various experimental conditions. The Curosurf® viscosity is found to increase exponentially with lipid concentration following the Krieger-Dougherty law of colloids. The Krieger-Dougherty behavior also predicts a divergence of the viscosity at the liquid-to-gel transition. For Curosurf® the transition concentration is found close to the concentration at which it is formulated (117 g L -1 versus 80 g L -1 ). This outcome suggests that for SRT the surfactant rheological properties need to be monitored and kept within a certain range. The results found here could help in producing suspensions for respiratory distress syndrome adapted to adults. The present work also demonstrates the potential of the magnetic wire microrheology technique as an accurate tool to explore biological soft matter dynamics.

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Section: Iii1 -Curosurf® Structuresupporting

confidence: 86%

On the rheology of pulmonary surfactant: Effects of concentration and consequences for the surfactant replacement therapy

Thai

Mousseau

Oikonomou

et al. 2019

Colloids and Surfaces B: Biointerfaces

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“…9c). Similar structures were observed on surfactant substitutes 28 and on polymer-liposome complexes. 29 Note that on the figure the Al 2 O 3 particles are not visible, probably because of their weak contrast as compared to that of the stained vesicles.…”

Section: Figure 7: Integrals Over the Mixing Ratio ! Between The Scatsupporting

confidence: 77%

“…6,26 Bulk samples were examined using freeze-fracture and (cryo)-transmission electron microscopy to determine the size and shape distribution of MLVs, and the organization of bilayers in the presence of biological or synthetic additives. [27][28][29] Previous studies focused on interfacial and rheological properties of surface layers, as it is relevant to its biophysical function in the lungs. 19,[30][31][32][33][34][35] In the alveoli however, the pulmonary surfactant forms a layer of a few hundreds of nanometers, 1,36 and after crossing the air-liquid interface the nanoparticles will also interact with the phospholipid sub-phase.…”

Section: -Introductionmentioning

confidence: 99%

Biophysicochemical Interaction of a Clinical Pulmonary Surfactant with Nanoalumina

et al. 2015

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We report on the interaction of pulmonary surfactant composed of phospholipids and proteins with nanometric alumina (Al 2 O 3 ) in the context of lung exposure and nanotoxicity. We study the bulk properties of phospholipid/nanoparticle dispersions and determine the nature of their interactions. The clinical surfactant Curosurf®, both native and extruded, and a protein-free surfactant are investigated. The phase behavior of mixed surfactant/particle dispersions was determined by optical and electron microscopy, light scattering and zeta potential measurements. It exhibits broad similarities with that of strongly interacting nanosystems such as polymers, proteins or particles, and supports the hypothesis of electrostatic complexation. At a critical stoichiometry, micron sized aggregates arising from the association between oppositely charged vesicles and nanoparticles are formed. Contrary to the models of lipoprotein corona or of particle wrapping, our work shows that vesicles maintain their structural integrity and trap the particles at their surfaces. The agglomeration of particles in surfactant phase is a phenomenon of importance since it could change the interactions of the particles with lung cells. keywords:Pulmonary surfactant -Curosurf® -Aluminum nanoparticles -Electrostatic complexationMultilamellar vesicles Corresponding authors: jean-francois.berret@univ-paris-diderot.fr Accepted at Langmuir: Tuesday, June 16, 2015 I -Introduction Pulmonary surfactant, the fluid lining the epithelium of the lungs is a complex surface-active fluid that contains phospholipids and lipids (85% and 5%, respectively) and 10% proteins (SP-A, SP-B, SP-C, SP-D and serum proteins). 1-2 The biophysical functions of pulmonary surfactant are to prevent the collapse of small alveoli during expiration and the overexpansion of large alveoli during inspiration. It also preserves bronchiolar patency during normal and forced respiration. 1,[3][4] Furthermore, it has an important immunological role of protecting the lungs from injuries and infections caused by inhaled particles, including microorganisms, particulate matter or engineered particles. [5][6][7][8][9][10] More specifically, particles of sizes less than 100 nm end up significantly deposited in the alveoli, and are susceptible to interact with the lung fluid. [11][12] To evaluate the risks of exposure to inhaled nanomaterials, recent studies have been focusing on the interaction of particles with membranes, more specifically on model systems made of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) or DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) unilamellar vesicles. [13][14][15][16][17][18][19][20] The review of the different interaction potentials ! 2! between particles and membranes revealed the importance of the interplay between particle/vesicle attraction and bilayer bending energy. 17 For diameters lower than a critical size (order of 10 nm for silica), the particles decorate the outer surface of the membrane, and induce aggregation. 17-18 For larger particle diamet...

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“…Most current conventional techniques, used to study microparticles, cannot analyse properly and fully MPs, or vesicles and liposome morphology [7], [8]. Even flow cytometry, which is the most common method to study MPs, offers a lower detection limit of only 300–500 nm [9], [10].…”

Section: Introductionmentioning

confidence: 99%

Cryogenic Transmission Electron Microscopy Nanostructural Study of Shed Microparticles

et al. 2013

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Microparticles (MPs) are sub-micron membrane vesicles (100–1000 nm) shed from normal and pathologic cells due to stimulation or apoptosis. MPs can be found in the peripheral blood circulation of healthy individuals, whereas elevated concentrations are found in pregnancy and in a variety of diseases. Also, MPs participate in physiological processes, e.g., coagulation, inflammation, and angiogenesis. Since their clinical properties are important, we have developed a new methodology based on nano-imaging that provides significant new data on MPs nanostructure, their composition and function. We are among the first to characterize by direct-imaging cryogenic transmitting electron microscopy (cryo-TEM) the near-to-native nanostructure of MP systems isolated from different cell types and stimulation procedures. We found that there are no major differences between the MP systems we have studied, as most particles were spherical, with diameters from 200 to 400 nm. However, each MP population is very heterogeneous, showing diverse morphologies. We investigated by cryo-TEM the effects of standard techniques used to isolate and store MPs, and found that either high-g centrifugation of MPs for isolation purposes, or slow freezing to –80°C for storage introduce morphological artifacts, which can influence MP nanostructure, and thus affect the efficiency of these particles as future diagnostic tools.

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Nanostructure of the aqueous form of lung surfactant of different species visualized by cryo‐transmission electron microscopy

Cited by 20 publications

References 24 publications

On the rheology of pulmonary surfactant: Effects of concentration and consequences for the surfactant replacement therapy

On the rheology of pulmonary surfactant: Effects of concentration and consequences for the surfactant replacement therapy

Biophysicochemical Interaction of a Clinical Pulmonary Surfactant with Nanoalumina

Cryogenic Transmission Electron Microscopy Nanostructural Study of Shed Microparticles

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