Nanostructured Carbon Beads—Properties and Biomedical Applications

Stanishevsky, Andrei; Styres, Courtney; Yockell-Lelièvre, Hélène; Yusuf, Nabiha

doi:10.1166/jnn.2011.3500

Cited by 4 publications

(2 citation statements)

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“…The bands between 1513 and 1485 cm −1 were attributed to phenyl and pterin rings (Zhang et al 2008). In turn, the FTIR spectrum of DOX indicated characteristic bands at 1730 cm −1 (C=O stretch, ketone), 1630 cm −1 (C=O stretch), 1282.7 cm −1 (C−O−C, stretch), 1114 cm −1 (C−O stretch, tertiary alcohol), 1070 cm −1 (C−O stretch, secondary alcohol), and 988 cm −1 (C−O stretch, primary alcohol) (Kanwal et al 2019;Stanishevsky et al 2011). The FTIR spectrum of the QD-FA nanocarriers presented in Fig.…”

Section: Qualitative Features Of the Ftir Spectra Of The Qd-fa Nanocamentioning

confidence: 99%

Quantum dots as targeted doxorubicin drug delivery nanosystems in human lung cancer cells

et al. 2021

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Background Lung cancer is one of the most frequently diagnosed cancers all over the world and is also one of the leading causes of cancer-related mortality. The main treatment option for small cell lung cancer, conventional chemotherapy, is characterized by a lack of specificity, resulting in severe adverse effects. Therefore, this study aimed at developing a new targeted drug delivery (TDD) system based on Ag–In–Zn–S quantum dots (QDs). For this purpose, the QD nanocrystals were modified with 11-mercaptoundecanoic acid (MUA), L-cysteine, and lipoic acid decorated with folic acid (FA) and used as a novel TDD system for targeting doxorubicin (DOX) to folate receptors (FARs) on adenocarcinomic human alveolar basal epithelial cells (A549). NIH/3T3 cells were used as FAR-negative controls. Comprehensive physicochemical, cytotoxicity, and genotoxicity studies were performed to characterize the developed novel TDDs. Results Fourier transformation infrared spectroscopy, dynamic light scattering, and fluorescence quenching confirmed the successful attachment of FA to the QD nanocrystals and of DOX to the QD–FA nanocarriers. UV–Vis analysis helped in determining the amount of FA and DOX covalently anchored to the surface of the QD nanocrystals. Biological screening revealed that the QD–FA–DOX nanoconjugates had higher cytotoxicity in comparison to the other forms of synthesized QD samples, suggesting the cytotoxic effect of DOX liberated from the QD constructs. Contrary to the QD–MUA–FA–DOX nanoconjugates which occurred to be the most cytotoxic against A549 cells among others, no such effect was observed for NIH/3T3 cells, confirming FARs as molecular targets. In vitro scratch assay also revealed significant inhibition of A549 cell migration after treatment with QD–MUA–FA–DOX. The performed studies evidenced that at IC50 all the nanoconjugates induced significantly more DNA breaks than that observed in nontreated cells. Overall, the QD–MUA–FA–DOX nanoconjugates showed the greatest cytotoxicity and genotoxicity, while significantly inhibiting the migratory potential of A549 cells. Conclusion QD–MUA–FA–DOX nanoconjugates can thus be considered as a potential drug delivery system for the effective treatment of adenocarcinomic human alveolar basal epithelial cells.

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Section: Qualitative Features Of the Ftir Spectra Of The Qd-fa Nanocamentioning

confidence: 99%

Quantum dots as targeted doxorubicin drug delivery nanosystems in human lung cancer cells

et al. 2021

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“…The bands between 1513 and 1485 cm − 1 are attributed to band of the phenyl and pterin ring. 23 In turn, the FTIR spectrum of DOX indicated characteristics bands at 1730 cm − 1 (C = O stretch, ketone), 1630 cm − 1 (C = O stretch), 1282.7 cm − 1 (C − O−C, stretch), 1114 cm − 1 (C − O stretch, tertiary alcohol), 1070 cm − 1 (C − O stretch, secondary alcohol), and 988 cm − 1 (C − O stretch, primary alcohol). 24,25 The FTIR spectrum of QD-FA nanocarriers presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

Quantum dots as targeted doxorubicin drug delivery nanosystems in human lung cancer cells

Ruzycka-Ayoush

Kowalik

Kowalczyk

et al. 2020

Preprint

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Background Lung cancer is one of the most frequently diagnosed cancer all over the world and a leading cancer-related mortality. The therapy of lung cancer includes surgery, chemotherapy and radiatherapy and mailny depends on the type and stage of lung cancer characterized based on WHO guidelines. Althought the conventional chemotherapy is the main treatment option for small cell lung cancer (SCLC) and a common treatment for non-SCLC it is characterized with lack of specificity resulting to severe toxicities of normal cells and harmful side effects. Therefore, targeted drug delivery (TDD) systems have been used to reduce the systemic toxicity of some conventional chemotherapies in lung cancer. Quantum dots (QDs) are fascinating nanoscale crystals that can serve as nanocarriers in TDD due to their unique physicochemical properties. Therefore, in this paper, the as-desiged QDs, Ag-In-Zn-S-based nanoconjugates for selective doxorubicin (DOX) targeting to lung cancer cells were developed. The QD nanocrystals were modified with 11-mercaptoundecanoic acid (MUA), L-cysteine (Cys) and lipoic acid (LA) used as drug carriers for targeted delivery of DOX to A549 cells through conjugated folic acid (FA) a self-navigation molecule that docks to the folate receptors on cancer cells. The comprechensive physicochemical, cytotoxicity and genotoxicity studies were performed to characterise the novel QD-based nanocaries and their anticancer cargos. Results The results from FTIR, DLS and fluorescence quenching evidenced the successful attachment of FA to the QDs nanocrystals and DOX to the QDs-FA nanocarriers. UV-vis analysis determined the amount of FA and DOX covalently anchored to the QDs nanocrystal surface. Biological screeining revealed that QDs-FA-DOX nanoconjugates showed higher cytotoxicity in comparison to other forms of the synthesized QD samples, suggesting the cytotoxic effect of liberated DOX from the QD constructs. QD-MUA-FA-DOX occurred to be the most cytotoxic against A549 cells among nanoconjugates. In vitro scratch assay also revealed significant inhibition of A549 migration only due to treatment with QD-MUA-FA-DOX. Studies evidenced that all the nanoconjugates at IC 50 induced significantly more DNA breaks than that observed in non-treated cells. All in all, significant and the greatest cytotoxicity, genotoxicity together with inhibition of migratory potential of A549 cells was observed for QD-MUA-FA-DOX. Conclusion The studies show the therapeutic efficacy of DOX-loaded QD-based cargos suggesting their promising role as novel drug delivery systems navigating to folate receptors in lung cancer cells.

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