Nanostructured cerium oxide film for triglyceride sensor

Solanki, Pratima R.; Dhand, Chetna; Kaushik, Ajeet; Ansari, Anees A.; Sood, K.N.; Malhotra, Bansi D.

doi:10.1016/j.snb.2009.05.034

Cited by 91 publications

(46 citation statements)

References 23 publications

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“…The value of current response decreases by about 14, 7, and 4 % upon the addition of AA, urea, and LA, respectively which confirms that the bioelectrode is highly selective. These phenomena are in good accordance with the previous report [63,64].…”

Section: Selectivity In Terms Of Interference Studysupporting

confidence: 94%

Nano-CeO2 decorated graphene based chitosan nanocomposites as enzymatic biosensing platform: fabrication and cellular biocompatibility assessment

Mohanty

Nayak

2015

Bioprocess Biosyst Eng

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The present study summarizes the designing of a green transducer phase based on nano-cerium oxide (CeO2) decorated reduced graphene oxide (RGO) reinforced chitosan nanocomposites as an effective enzyme immobilizer and bio-sensing matrix for glucose analyte. Also, it scrutinizes the biocompatibility and cell viability of the synthesized nanohybrid with human fibroblastic macrophage cell line. CeO2 nanoparticles (NPs) were successfully grown on graphene nanosheet in the presence of cationic surfactant followed by facile hydrothermal treatment. The eventual growth of synthesized CeO2 nanocrystals on the graphene layer was confirmed from X-ray diffraction (XRD), transmission electron microscopy (TEM) and Raman analysis. The biocompatibility of the synthesized nanohybrid was also evident from the MTT assay. Glucose oxidase (GOx) was employed on the green polymer nanocomposites modified FTO electrode to fabricate an enzymatic bioelectrode. The electroanalytical response of the GOx/nano-CeO2/RGO/CS/FTO bioelectrode towards electrooxidation of glucose analyte was investigated by electrochemical impedance (EIS) and cyclic voltammetry (CV) study. The resulting biosensor exhibited a good electrochemical response to glucose within the linear detection range of 0.05-6.5 mM with a low detection limit of 2 μM and a sensitivity of 7.198 μA mM(-1) cm(-2). The bioelectrode also showed good shelf life (~10 weeks) and negligible interfering ability under controlled environment. The obtained results indicate that nano-CeO2/RGO nanohybrid based chitosan nanocomposites achieve a biocompatible biosensing platform for effective enzyme immobilization due to the excellent synergistic effects between the CeO2 nanoparticles and graphene sheet.

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Section: Selectivity In Terms Of Interference Studysupporting

confidence: 94%

Nano-CeO2 decorated graphene based chitosan nanocomposites as enzymatic biosensing platform: fabrication and cellular biocompatibility assessment

Mohanty

Nayak

2015

Bioprocess Biosyst Eng

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“…Besides this, presence of acid bioanalytes i.e. uric acid, lactic acid, ascorbic acid etc can interfere with the triglyceride response [23].…”

mentioning

confidence: 99%

Polyaniline/Single‐Walled Carbon Nanotubes Composite Based Triglyceride Biosensor

et al. 2010

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Nanocomposite film comprising of polyaniline (PANI) and single walled carbon nanotubes (SWCNT) has been fabricated onto indium-tin-oxide (ITO) coated glass plate using electrophoretic technique. Co-immobilization of glycerol dehydrogenase (GDH) and lipase (LIP) has been done via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry to explore its application for triglyceride (tributyrin) sensing. Response studies have been done using linear sweep voltammetry revealing that LIP-GDH/PANI-SWCNT-TB/ITO bioelectrode can detect tributyrin in the range of 50 to 400 mg dL À1 with low Michaelis-Menten constant of 1.138 mM, improved response time of 12 s, high sensitivity as 4.28 10 À4 mA mg À1 dL and storage stability of about 13 weeks.

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“…Th is biosensor exhibits good linearity, low detection limit, long shelf-life and low K m (22.27 mg dL -1 ). A lipase-based biolectrode of nanostructured CeO 2 and indium tin oxide (ITO) prepared by Solanki et al [47] similarly shows high affi nity for tributyrin [47]. Eff orts should be made to utilize these electrodes in NMO-based biosensing devices for estimation of triglyceride concentrations in blood.…”

Section: Lipasementioning

confidence: 99%

Nanostructured metal oxide-based biosensors

et al. 2011

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A mong the various types of nanomaterials that have been developed, nanostructured metal oxides (NMOs) have recently aroused much interest as immobilizing matrices for biosensor development ( Figure 1) [1][2][3][4][5][6][7][8][9]. Nanostructured oxides of metals such as zinc, iron, cerium, tin, zirconium, titanium, metal and magnesium have been found to exhibit interesting nanomorphological, functional biocompatible, non-toxic and catalytic properties. Th ese materials also exhibit enhanced electron-transfer kinetics and strong adsorption capability, providing suitable microenvironments for the immobilization of biomolecules and resulting in enhanced electron transfer and improved biosensing characteristics. Various morphologies of NMOs have been obtained using a variety of methods, including soft templating for the preparation of nanorods and nanofi bers [10], sol-gel methods for the production of three-dimensional (3D) ordered rough nanostructures [6,11], radiofrequency sputtering for rough nanostructures [4] and hydrothermal deposition for nanoparticles with controlled shape [12]. All of these NMOs have been reported to have potential applications in biosensors. Recently, the optical, electrical and magnetic properties of NMOs have been reported to be enhanced through the incorporation of nanoparticles of conducting or semiconducting materials such as carbon nanotubes, graphene, gold and silver, as well as quantum dots of various semiconductors, with advantages for improved biosensor characteristics [13][14][15][16]. It is expected that the judicious application of an NMO may lead to the fabrication of novel biosensing devices with enhanced signal amplifi cation and coding strategies for bioaffi nity assays and effi cient electrical communication with redox biomolecules/ enzymes that may address future diagnostic needs.Th e unique properties of NMOs off er excellent prospects for interfacing biological recognition events with electronic signal transduction and for designing a new generation of bioelectronics devices that may exhibit novel functions. Th e controlled preparation of an NMO is considered to play a signifi cant role in the development of biosensors. Eff orts are being made to explore the prospects and future challenges of NMOs for the development of biosensing devices and the evolution of new strategies for bioaffi nity assays and effi cient electrical communication. In this review, we focus on developments over the past fi ve years in NMO-based biosensors for clinical and non-clinical applications (Figure 2). Fundamentals of nanostructured metal oxides for biosensingAmong the various immobilizing matrices that have been developed, NMOs have exceptional optical and electrical properties due to electron and phonon confi nement, high surface-to-volume ratios, modifi ed surface work function, high surface reaction activity, high catalytic effi ciency and strong adsorption ability. For these reasons, NMOs have been utilized to increase the loading of biomolecules per Nanostructured metal oxide-based b...

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Nanostructured cerium oxide film for triglyceride sensor

Cited by 91 publications

References 23 publications

Nano-CeO2 decorated graphene based chitosan nanocomposites as enzymatic biosensing platform: fabrication and cellular biocompatibility assessment

Nano-CeO2 decorated graphene based chitosan nanocomposites as enzymatic biosensing platform: fabrication and cellular biocompatibility assessment

Polyaniline/Single‐Walled Carbon Nanotubes Composite Based Triglyceride Biosensor

Nanostructured metal oxide-based biosensors

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