The purpose. To increase selfdescriptiveness of the method of biotesting waters of surface sources of water service and drinking water due to use of fishes Danio rerio (Brachydanio). Methods. Biotesting with use of the test-reaction of fixation of fishes Poeciliia reticulata Peters and Danio rerio Hamilton-Buchanan with 8-diurnal exposure of individuals. Results. During biotesting at absence of fixation of fishes Poeciliia reticulata Peters toxicity of examined waters is not determined, and on the basis of a fixation of fishes Danio rerio Hamilton-Buchanan the low level of toxicity of waters in the range of 25-50% is fixed. Conclusions. It is expedient to make assessment of chronic toxicity of waters of surface sources of water service and drinking water by method of biotesting on fishes Danio rerio Hamilton-Buchanan, which have appeared more sensitive to low levels of pollutions (on the average on 26,3% in acute 4-days experience, and on 36,6% in chronic 8-days experience), than traditional test-object of fish Poeciliia reticulata Peters.