Nanostructured Materials as Biosensor Transducers: Achievements and Future Developments

Starodub, Nickolaj F.; Shavanova, K.; Shpyrka, N. F.; Melnichenko, M. M.; Viter, Roman

doi:10.1002/9781119314196.ch10

Cited by 2 publications

(1 citation statement)

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“…It is known, that UV band of ZnO-NRs can be tailored by the variation of depleted layer width, which is controlling photo-induced charge separation in near interphase between ZnO and solution or air [25]. As it is reported previously in our recent reviews on metal oxide biosensors, there is still no very solid explanation for the mechanism of interaction between biomolecules and ZnO surface [15,18,23,28]. Therefore, the interaction mechanism at some extent can be assessed by the evaluation of direct and polymer assisted immobilization of biomolecules on the ZnO-NRs surface.…”

Section: Testing Of Optical Immunosensor Based On Zno-nrsmentioning

confidence: 97%

Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

Viter

Savchuk

Стародуб

et al. 2019

Sensors and Actuators B: Chemical

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Bovine leukaemia virus (BLV) proteins gp51, which are serving as antigens for specific antibodies against BLV proteins (anti-gp51), were applied as biological recognition part in the design of immunosensor devoted for the determination of anti-gp51. The efficiency of the immobilization of BLV proteins gp51 on ZnO nanorod (ZnO-NR) modified glass (ZnO-NR/glass) surface was evaluated. The formation of antigen-antibody complex on the ZnO/glass modified by the BLV proteins gp51 (gp51/ZnO-NR/glass) was investigated by the determination of changes in ZnO photoluminescence. The applicability of gp51/ZnO-NR/glass in the design of photoluminescence based immunosensor was evaluated. Bovine serum albumin (BSA) was applied for the modification of sensing gp51 layer in order to form gp51&BSA layer with advanced selectivity. Polyallylamine hydrochloride (PAH) was applied in order to improve the immobilization of gp51 and BSA based sensing layer (gp51&BSA) on the surface of ZnO-NR/glass. PAH was applied during the formation of gp51&BSA/PAH/ZnO-NR/glass structure. Some aspects of the mechanism of interaction between biomolecules (gp51, BSA and anti-gp51) and ZnO-NR during the preparation and action of gp51&BSA/ZnO-NR/glass-and gp51&BSA/PAH/ZnO-NR/glass-based immunosensors have been discussed.

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