Nanostructured TiO2 Surfaces Promote Human Bone Marrow Mesenchymal Stem Cells Differentiation to Osteoblasts

Vercellino, Marco; Ceccarelli, Gabriele; Cristofaro, Francesco; Balli, Martina; Bertoglio, Federico; Bruni, Gianna; Benedetti, Laura; Avanzini, Maria Antonietta; Imbriani, Marcello; Visai, Livia

doi:10.3390/nano6070124

Cited by 24 publications

(25 citation statements)

References 51 publications

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“…this work we have studied the hBM-MSCs differentiation process assessing the possibility to control the space and fate rate by exogenously applied nHap. The addition of nHap in the cell culture increased the synthesis and deposition of ALP, OCN, DCNand Col-III, promoting a greater deposition of these proteins, which are strictly related to the ECM mineralization 16. To confirm the close relationship between bone matrix proteins and mineral deposits, OCN and DCN resulted more concentrated in specific areas of the samples, likely in correspondence to the hydroxyapatite deposits as already reported by other authors 32.…”

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confidence: 71%

Heterogeneous and self-organizing mineralization of bone matrix promoted by hydroxyapatite nanoparticles

et al. 2017

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The mineralization process is crucial to the load-bearing characteristics of the bone extracellular matrix. In this work, we have studied the spatiotemporal dynamics of mineral deposition by human bone marrow mesenchymal stem cells differentiating toward osteoblasts promoted by the presence of exogenous hydroxyapatite nanoparticles. At the molecular level, the added nanoparticles positively modulated the expression of bone-specific markers and enhanced calcified matrix deposition during osteogenic differentiation. The nucleation, growth and spatial arrangement of newly deposited hydroxyapatite nanocrystals have been evaluated using scanning micro X-ray diffraction and scanning micro X-ray fluorescence. As leading results, we have found the emergence of a complex scenario where the spatial organization and temporal evolution of the process exhibit heterogeneous and self-organizing dynamics. At the same time the possibility of controlling the differentiation kinetics, through the addition of synthetic nanoparticles, paves the way to empower the generation of more structured bone scaffolds in tissue engineering and to design new drugs in regenerative medicine.

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confidence: 71%

Heterogeneous and self-organizing mineralization of bone matrix promoted by hydroxyapatite nanoparticles

et al. 2017

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“…Therefore, biomaterial interactions with stem cells are critical for the long-term success of medical devices. In practice the most widely used source of stem cells is bone marrow and the stromal vascular fraction (SVF) of adipose tissue [40]. The collection procedure of bone marrow is painful and provides a limited amount of multipotent cells [41][42][43], however, the second collection procedure has gained more and more attention.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Studies on Nanoporous, Nanotubular and Nanosponge-Like Titania Coatings, with the Use of Adipose-Derived Stem Cells

et al. 2020

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In vitro biological research on a group of amorphous titania coatings of different nanoarchitectures (nanoporous, nanotubular, and nanosponge-like) produced on the surface of Ti6Al4V alloy samples have been carried out, aimed at assessing their ability to interact with adipose-derived mesenchymal stem cells (ADSCs) and affect their activity. The attention has been drawn to the influence of surface coating architecture and its physicochemical properties on the ADSCs proliferation. Moreover, in vitro co-cultures: (1) fibroblasts cell line L929/ADSCs and (2) osteoblasts cell line MG-63/ADSCs on nanoporous, nanotubular and nanosponge-like TiO2 coatings have been studied. This allowed for evaluating the impact of the surface properties, especially roughness and wettability, on the creation of the beneficial microenvironment for co-cultures and/or enhancing differentiation potential of stem cells. Obtained results showed that the nanoporous surface is favorable for ADSCs, has great biointegrative properties, and supports the growth of co-cultures with MG-63 osteoblasts and L929 fibroblasts. Additionally, the number of osteoblasts seeded and cultured with ADSCs on TNT5 surface raised after 72-h culture almost twice when compared with the unmodified scaffold and by 30% when compared with MG-63 cells growing alone. The alkaline phosphatase activity of MG-63 osteoblasts co-cultured with ADSCs increased, that indirectly confirmed our assumptions that TNT-modified scaffolds create the osteogenic niche and enhance osteogenic potential of ADSCs.

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“…The morphology of TiO 2 nanomaterials (i.e., nanotubes) is, nevertheless, the most important factor in improving cell adhesion, proliferation, and differentiation [35,36]. A scaffold composed of polylactic-co-glycolic acid (PLGA) and TiO 2 nanoparticles as well as decorated glass with TiO 2 nanoparticles can improve the amount of precipitated calcium for bone regeneration compared to the scaffold without TiO 2 nanoparticles [206,207]. The adhesion and spreading of osteoblast cells with a complete integration can also be attained with composites made of polylactic acid (PLA), poly-ε-caprolactone (PCL), and TiO 2 particles or nanofiber meshes mimicking the bone regeneration properties [37,38].…”

Section: Tissue Regeneration and Chronic Wound Healingmentioning

confidence: 99%

Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine

Kafshgari

Goldmann

2020

Nano-Micro Lett.

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HIGHLIGHTS • Multifunctional TiO 2 nanostructures hold promise for advancing a wide range of biomedical applications due to a feasible integration of distinct theranostic features. • Fabrication and post-fabrication strategies implemented to generate multifunctional TiO 2 nanostructures for a broad range of biomedical applications are briefly outlined. The opportunities and challenges of TiO 2 nanomaterials are highlighted in order to open the possibility of clinical translation. ABSTRACT Titanium dioxide (TiO 2 ) nanostructures exhibit a broad range of theranostic properties that make them attractive for biomedical applications. TiO 2 nanostructures promise to improve current theranostic strategies by leveraging the enhanced quantum confinement, thermal conversion, specific surface area, and surface activity. This review highlights certain important aspects of fabrication strategies, which are employed to generate multifunctional TiO 2 nanostructures, while outlining post-fabrication techniques with anemphasis on their suitability for nanomedicine. The biodistribution, toxicity, biocompatibility, cellular adhesion, and endocytosis of these nanostructures, when exposed to biological microenvironments, are examined in regard to their geometry, size, and surface chemistry. The final section focuses on recent biomedical applications of TiO 2 nanostructures, specifically evaluating therapeutic delivery, photodynamic and sonodynamic therapy, bioimaging, biosensing, tissue regeneration, as well as chronic wound healing.

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Nanostructured TiO2 Surfaces Promote Human Bone Marrow Mesenchymal Stem Cells Differentiation to Osteoblasts

Cited by 24 publications

References 51 publications

Heterogeneous and self-organizing mineralization of bone matrix promoted by hydroxyapatite nanoparticles

Heterogeneous and self-organizing mineralization of bone matrix promoted by hydroxyapatite nanoparticles

In Vitro Studies on Nanoporous, Nanotubular and Nanosponge-Like Titania Coatings, with the Use of Adipose-Derived Stem Cells

Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine

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