Nanostructured TNFα protein targets the zebrafish (Danio rerio) immune system through mucosal surfaces and improves the survival after Mycobacterium marinum lethal infection

Ji, Jie; Torrealba, Débora; Thwaite, Rosemary; Gómez, Andromeda-Celeste; Parra, David; Roher, Nerea

doi:10.1016/j.aquaculture.2019.05.050

Cited by 9 publications

(7 citation statements)

References 45 publications

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“…Many variables for vaccine efficacy are present and should be considered when conducting immersion vaccination trials [2]. These include vaccine (antigen) dose, duration of immersion, particulate/soluble antigen uptake during immersion immunization of fish [3], adjuvant performance [4][5][6][7][8][9], temperature [10], fish size (age) [10,11], osmolarity, prime boost strategy [1], mucosal integrity [12,13], replicative vs. non-replicative vaccines [1] and how the experimental pathogen challenges are carried out (e.g., virulence of the challenge pathogen, high or low pathogen pressure/load).…”

Section: Introductionmentioning

confidence: 99%

“…This modality increased vaccine efficacy compared to what was obtained using naked vaccine antigens [17]. The other one used TNF alpha (TNF-α) nanoparticles which hold promise as an adjuvant for immersion vaccination [4]. Further on, recent studies suggest that nanoliposomes [18], recombinant live viruses expressing protective antigens, attenuated live vaccines [19][20][21][22] and microbubbles [13] may be used to increase the vaccine efficacy of immersion vaccines.…”

Section: Introductionmentioning

confidence: 99%

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Review on Immersion Vaccines for Fish: An Update 2019

Bøgwald

Dalmo

2019

Microorganisms

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Immersion vaccines are used for a variety of aquacultured fish to protect against infectious diseases caused by bacteria and viruses. During immersion vaccination the antigens are taken up by the skin, gills or gut and processed by the immune system, where the resulting response may lead to protection. The lack of classical secondary responses following repeated immersion vaccination may partly be explained by the limited uptake of antigens by immersion compared to injection. Administration of vaccines depends on the size of the fish. In most cases, immersion vaccination is inferior to injection vaccination with regard to achieved protection. However, injection is problematic in small fish, and fry as small as 0.5 gram may be immersion vaccinated when they are considered adaptively immunocompetent. Inactivated vaccines are, in many cases, weakly immunogenic, resulting in low protection after immersion vaccination. Therefore, during recent years, several studies have focused on different ways to augment the efficacy of these vaccines. Examples are booster vaccination, administration of immunostimulants/adjuvants, pretreatment with low frequency ultrasound, use of live attenuated and DNA vaccines, preincubation in hyperosmotic solutions, percutaneous application of a multiple puncture instrument and application of more suitable inactivation chemicals. Electrostatic coating with positively charged chitosan to obtain mucoadhesive vaccines and a more efficient delivery of inactivated vaccines has also been successful.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Review on Immersion Vaccines for Fish: An Update 2019

Bøgwald

Dalmo

2019

Microorganisms

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“…Those authors proved that the M. marinum infection model in adult zebrafish was suitable for preclinical screening of tuberculosis immune's responses and vaccines. It was also a promising new model for tuberculosis vaccine research, including the pre-clinical identification of vaccine antigens [16,17,36,41,61,67];). Other species of Mycobacterium have also been studied, such as M. bovis [52,73] and M. abscessos [7].…”

Section: Animals and Human Vaccinesmentioning

confidence: 99%

Zebrafish as an alternative animal model in human and animal vaccination research

et al. 2020

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Much of medical research relies on animal models to deepen knowledge of the causes of animal and human diseases, as well as to enable the development of innovative therapies. Despite rodents being the most widely used research model worldwide, in recent decades, the use of the zebrafish (Danio rerio) model has exponentially been adopted among the scientific community. This is because such a small tropical freshwater teleost fish has crucial genetic, anatomical and physiological homology with mammals. Therefore, zebrafish constitutes an excellent experimental model for behavioral, genetic and toxicological studies which unravels the mechanism of various human diseases. Furthermore, it serves well to test new therapeutic agents, such as the safety of new vaccines. The aim of this review was to provide a systematic literature review on the most recent studies carried out on the topic. It presents numerous advantages of this type of animal model in tests of efficacy and safety of both animal and human vaccines, thus highlighting gains in time and cost reduction of research and analyzes.

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“…Proteins from the host immune system, such as antimicrobial peptides, cytokines, chemokines, and hormones, have been explored as immunomodulators due to their efficacy in modulating immune pathways [ 15 , 16 , 17 ]. Antimicrobial peptides (AMPs) play a role in host defense and are promising therapeutic agents [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…In salmonids, hepcidin has shown antimicrobial activity against pathogens such as Piscirikettsia salmonis , A. salmonicida , Aeromonas hydrophila , and Pseudomonas aeruginosa [ 26 , 27 ]. Cytokines as IBs have been previously evaluated in fish, where TNFα was tested as an immunostimulant in rainbow trout and zebrafish ( Danio rerio ) [ 15 , 16 ], and in pigs, where IL1, IL6, IL8, and TNFα were tested as stimulators of the intestinal mucosa [ 31 ]. To the best of our knowledge, although AMP-based IBs have been described [ 18 ], no work on teleost IBs of AMPs has been published.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells

Torrealba,

López,

Zelada

et al. 2024

Animals

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Recent studies have demonstrated that immune-related recombinant proteins can enhance immune function, increasing host survival against infectious diseases in salmonids. This research evaluated inclusion bodies (IBs) of antimicrobial peptides (CAMPIB and HAMPIB) and a cytokine (IL1βIB and TNFαIB) as potential immunostimulants in farmed salmonids. For this purpose, we produced five IBs (including iRFPIB as a control), and we evaluated their ability to modulate immune marker gene expression of three IBs in the RTS11 cell line by RT–qPCR. Additionally, we characterized the scale-up of IBs production by comparing two different scale systems. The results showed that CAMPIB can increase the upregulation of tnfα, il1β, il8, and il10, HAMPIB significantly increases the upregulation of tnfα, inos, and il10, and IL1βIB significantly upregulated the expression of tnfα, il1β, and cox2. A comparison of IL1βIB production showed that the yield was greater in shake flasks than in bioreactors (39 ± 1.15 mg/L and 14.5 ± 4.08 mg/L), and larger nanoparticles were produced in shake flasks (540 ± 129 nm and 427 ± 134 nm, p < 0.0001, respectively). However, compared with its shake flask counterpart, the IL1βIB produced in a bioreactor has an increased immunomodulatory ability. Further studies are needed to understand the immune response pathways activated by IBs and the optimal production conditions in bioreactors, such as a defined medium, fed-batch production, and mechanical bacterial lysis, to increase yield.

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Nanostructured TNFα protein targets the zebrafish (Danio rerio) immune system through mucosal surfaces and improves the survival after Mycobacterium marinum lethal infection

Cited by 9 publications

References 45 publications

Review on Immersion Vaccines for Fish: An Update 2019

Review on Immersion Vaccines for Fish: An Update 2019

Zebrafish as an alternative animal model in human and animal vaccination research

Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells

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