Nanotechnological solutions for controlling transmission and emergence of antimicrobial-resistant bacteria, future prospects, and challenges: a systematic review

Ssekatawa, Kenneth; Byarugaba, Dennis K.; Kato, Charles Drago; Ejobi, Francis; Tweyongyere, Robert; Lubwama, Michael; Kirabira, John Baptist; Wampande, Eddie M.

doi:10.1007/s11051-020-04817-7

Cited by 32 publications

(23 citation statements)

References 137 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently nanotechnology is applied to combat the current COVID-19 pandemic [55][56][57]. Nanoparticles (NPs) have been applied previously for the treatment of several viral infections with promising results [27,54,[58][59][60] and they were also demonstrated a good antibacterial activity against multidrug resistant bacteria [61][62][63]. Therefore, the previously isolated and characterized Rha(s) mixture was used to produce Rha(s) nano-micelles containing different concentrations of rhamnolipids (1, 5 and 10 mg mL −1 ).…”

Section: Discussionmentioning

confidence: 99%

Rhamnolipids Nano-Micelles as a Potential Hand Sanitizer

et al. 2021

View full text Add to dashboard Cite

COVID-19 is a pandemic disease caused by the SARS-CoV-2, which continues to cause global health and economic problems since emerging in China in late 2019. Until now, there are no standard antiviral treatments. Thus, several strategies were adopted to minimize virus transmission, such as social distancing, face covering protection and hand hygiene. Rhamnolipids are glycolipids produced formally by Pseudomonas aeruginosa and as biosurfactants, they were shown to have broad antimicrobial activity. In this study, we investigated the antimicrobial activity of rhamnolipids against selected multidrug resistant bacteria and SARS-CoV-2. Rhamnolipids were produced by growing Pseudomonas aeruginosa strain LeS3 in a new medium formulated from chicken carcass soup. The isolated rhamnolipids were characterized for their molecular composition, formulated into nano-micelles, and the antibacterial activity of the nano-micelles was demonstrated in vitro against both Gram-negative and Gram-positive drug resistant bacteria. In silico studies docking rhamnolipids to structural and non-structural proteins of SARS-CoV-2 was also performed. We demonstrated the efficient and specific interaction of rhamnolipids with the active sites of these proteins. Additionally, the computational studies suggested that rhamnolipids have membrane permeability activity. Thus, the obtained results indicate that SARS-CoV-2 could be another target of rhamnolipids and could find utility in the fight against COVID-19, a future perspective to be considered.

show abstract

Section: Discussionmentioning

confidence: 99%

Rhamnolipids Nano-Micelles as a Potential Hand Sanitizer

et al. 2021

View full text Add to dashboard Cite

show abstract

“…An alternative approach is to modify existing drugs to enhance their efficacy. In this regard, nanotechnology-based nanomedicine is a promising approach in eradicating bacterial infections (Mukheem et al 2018 ; Ssekatawa et al 2020 ). Nanoparticles (NPs) have demonstrated antibacterial activity against a variety of MDR bacteria by increasing the surface area, enhancing release of drug, reducing the dose required, and improving solubility and bioavailability of drugs (Wang et al 2017 ; Anwar et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Zinc oxide nanoparticles conjugated with clinically-approved medicines as potential antibacterial molecules

et al. 2021

View full text Add to dashboard Cite

At present, antibiotic resistance is one of the most pressing issues in healthcare globally. The development of new medicine for clinical applications is significantly less than the emergence of multiple drug-resistant bacteria, thus modification of existing medicines is a useful avenue. Among several approaches, nanomedicine is considered of potential therapeutic value. Herein, we have synthesized Zinc oxide nanoparticles (ZnO-NPs) conjugated with clinically-approved drugs (Quercetin, Ceftriaxone, Ampicillin, Naringin and Amphotericin B) with the aim to evaluate their antibacterial activity against several Gram-positive (Methicillin resistant Staphylococcus aureus, Streptococcus pneumoniae and Streptococcus pyogenes) and Gram-negative (Escherichia coli K1, Serratia marcescens and Pseudomonas aeruginosa) bacteria. The nanoparticles and their drug conjugates were characterized using UV-visible spectrophotometry, dynamic light scattering, Fourier transform infrared spectroscopy and atomic force microscopy. Antibacterial activity was performed by dilution colony forming unit method and finally 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were performed to determine their cytotoxic effects against human cell lines. ZnO-NPs revealed maxima surface plasmon resonance band at 374 and after conjugation with beta-cyclodextrin at 379 nm, polydispersity with size in range of 25–45 nm with pointed shaped morphology. When conjugated with ZnO-NPs, drug efficacy against MDR bacteria was enhanced significantly. In particular, Ceftriaxone- and Ampicillin-conjugated ZnO-NPs exhibited potent antibacterial effects. Conversely, ZnO-NPs and drugs conjugated NPs showed negligible cytotoxicity against human cell lines except Amphotericin B (57% host cell death) and Amphotericin B-conjugated with ZnO-NPs (37% host cell death). In conclusion, the results revealed that drugs loaded on ZnO-NPs offer a promising approach to combat increasingly resistant bacterial infections.

show abstract

“…Drug delivery systems with antimicrobial activity such as chitosan will not only shield antibiotics from the bacterial hydrolytic enzymes but also re-potentiate the antibiotic by conferring the drug delivery system-antibiotics complex synergistic bactericidal effect 19 . Furthermore, drug delivery systems augment sensitivity of MDR pathogens to antibiotics by circumventing resistance mechanisms such as impenetrability to antibiotics due to modification of the outer membrane porin proteins since the drug delivery system-antibiotic complex presents changed conformation compared with the non-encapsulated antibiotic 20 . Thus, isolation of chitosan from readily available resources with potential future applications as antimicrobials and chitosan-based drug delivery system to combat antimicrobial resistance is necessary.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of chitosan from Ugandan edible mushrooms, Nile perch scales and banana weevils for biomedical applications

Ssekatawa

Byarugaba

Wampande

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

Of recent, immense attention has been given to chitosan in the biomedical field due to its valuable biochemical and physiological properties. Traditionally, the chief source of chitosan is chitin from crab and shrimp shells. Chitin is also an important component of fish scales, insects and fungal cell walls. Thus, the aim of this study was to isolate and characterize chitosan from locally available material for potential use in the biomedical field. Chitosan ash and nitrogen contents ranged from 1.55 to 3.5% and 6.6 to 7.0% respectively. Molecular weight varied from 291 to 348KDa. FTIR spectra revealed high degree of similarity between locally isolated chitosan and commercial chitosan with DD ranging from 77.8 to 79.1%. XRD patterns exhibited peaks at 2θ values of 19.5° for both mushroom and banana weevil chitosan while Nile perch scales chitosan registered 3 peaks at 2θ angles of 12.3°, 20.1° and 21.3° comparable to the established commercial chitosan XRD pattern. Locally isolated chitosan exhibited antimicrobial activity at a very high concentration. Ash content, moisture content, DD, FTIR spectra and XRD patterns revealed that chitosan isolated from locally available materials has physiochemical properties comparable to conventional chitosan and therefore it can be used in the biomedical field.

show abstract

Nanotechnological solutions for controlling transmission and emergence of antimicrobial-resistant bacteria, future prospects, and challenges: a systematic review

Cited by 32 publications

References 137 publications

Rhamnolipids Nano-Micelles as a Potential Hand Sanitizer

Rhamnolipids Nano-Micelles as a Potential Hand Sanitizer

Zinc oxide nanoparticles conjugated with clinically-approved medicines as potential antibacterial molecules

Isolation and characterization of chitosan from Ugandan edible mushrooms, Nile perch scales and banana weevils for biomedical applications

Contact Info

Product

Resources

About