Nanotechnologies for biomedical science and translational medicine

Heath, James R.

doi:10.1073/pnas.1515202112

Cited by 86 publications

(52 citation statements)

References 128 publications

(104 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of NPs offers effective solutions to a wide range of biomedical issues 29 . Their entry into target cells is a fundamental step towards boosting therapeutic efficacy.…”

Section: Resultsmentioning

confidence: 99%

An apolipoprotein-enriched biomolecular corona switches the cellular uptake mechanism and trafficking pathway of lipid nanoparticles

et al. 2017

View full text Add to dashboard Cite

Following exposure to biological milieus (e.g. after systemic administration), nanoparticles (NPs) get covered by an outer biomolecular corona (BC) that defines many of their biological outcomes, such as the elicited immune response, biodistribution, and targeting capabilities. In spite of this, the BC role in regulating the cellular uptake and the subcellular trafficking properties of NPs has remained elusive. Here, we tackle this issue by employing multicomponent (MC) lipid NPs, human plasma (HP) and HeLa cells as models for nanoformulation, biological fluid, and target cell (respectively). By confocal fluorescence microscopy experiments and image correlation analyses, we quantitatively demonstrate that the BC promotes a neat switch of cell entry mechanism and subsequent intracellular trafficking, from macropinocytosis to clathrin-dependent endocytosis. Nano liquid chromatography tandem mass spectrometry identifies Apolipoproteins as the most abundant components of the BC tested here. Interestingly, this class of proteins target the LDL receptors, which are overexpressed in clathrin-enriched membrane domains. Our results highlight the crucial role of BC as an intrinsic trigger of specific NP-cell interactions and biological responses and set the basis for a rational exploitation of the BC for targeted delivery.

show abstract

“…The use of NPs offers effective solutions to a wide range of biomedical issues 29 . Their entry into target cells is a fundamental step towards boosting therapeutic efficacy.…”

Section: Resultsmentioning

confidence: 99%

An apolipoprotein-enriched biomolecular corona switches the cellular uptake mechanism and trafficking pathway of lipid nanoparticles

et al. 2017

View full text Add to dashboard Cite

show abstract

“…All of these “omics” techniques will generate massive amounts of quantitative experimental data, which will require advanced statistical and computational methods and systems biology approaches for successful integration [42]. In addition, the rapid progress of nanotechnology will undoubtedly complement and greatly empower the “omics” fields of medical research [43]. …”

Section: Discussionmentioning

confidence: 99%

PECAM-1 Leu125Val (rs688) Polymorphism and Diabetic Nephropathy in Caucasians with Type 2 Diabetes Mellitus

Završnik

Kariž

Makuc

et al. 2016

Analytical Cellular Pathology

View full text Add to dashboard Cite

Objectives. Platelet endothelial cell adhesion molecule-1 (PECAM-1) plays a key role in the transendothelial migration of circulating leukocytes during inflammation and in the maintenance of vascular endothelial integrity. We hypothesized that genetic variation in PECAM-1 gene could be associated with diabetic nephropathy (DN) and with the level of soluble PECAM-1 in Caucasians with type 2 diabetes mellitus (T2DM). Design and Methods. We analyzed the rs688 single nucleotide polymorphism of PECAM-1 gene C373G (Leu125Val) at exon 3, which encodes the first extracellular Ig-like domain that mediates the homophilic binding of PECAM-1, in 276 T2DM subjects with documented DN (cases) and 375 T2DM subjects without DN (controls), using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) strategy. Level of plasma soluble PECAM-1 (sPECAM-1) was measured by ELISA in a subpopulation of 120 diabetics with DN. Results. We found no association between the Leu125Val polymorphism and DN in subjects with T2DM. Likewise, the Leu125Val polymorphism was not associated with serum sPECAM-1 levels in a subpopulation of 120 diabetics with DN. Conclusion. The Leu125Val polymorphism of PECAM-1 and the level of sPECAM-1 are not associated with DN in T2DM subjects of Slovenian origin.

show abstract

“…Further progress is to be expected in these as well as in additional areas with major disruptive potential like single-cell biology and immunotherapy [126]. Since nanosystems operate in the same dimensional range as the fundamental units of life (from macromolecules to cells) and afford an exceptional flexibility of design and functions, it has been argued that Nanotechnology is the future of medicine [111].…”

Section: Nanotechnologies For Tissue Engineering and Wearables For Hementioning

confidence: 99%

Deployment and exploitation of nanotechnology nanomaterials and nanomedicine

Matteucci¹,

Giannantonio²,

Calabi³

et al. 2018

AIP Conference Proceedings

View full text Add to dashboard Cite

Abstract. Since around 50 years ago, the academic world is developing knowledge and technology to understand and to control matter at the nanoscale in order to exploit the peculiar mechanical, electrical, optical and magnetic properties emerging when a discrete number of atoms is assembled in structures which must be described according to the weird rules of quantum mechanics. This huge know how, commonly named Nanotechnology, was nucleated according to deployment strategies mainly defined and funded worldwide by governmental institutions in order to create the base for their further industrial exploitation and to provide an expectedly large socioeconomic impact. In the following, we will give a brief overview of the main applications of nanomaterials and an estimate of their value, based on the forecasts provided by some market research companies. In particular, we will briefly disclose the application of nanomaterials in the fields of Electronics & ICT, Energy and Environment, and, in particular, in the highly rewarding field of Nanomedicine. Further, we will highlight some key aspects of the deployment policies undertaken around the world which still are a key prerequisite for a full exploitation of the potential of Nanotechnology.

show abstract

Nanotechnologies for biomedical science and translational medicine

Cited by 86 publications

References 128 publications

An apolipoprotein-enriched biomolecular corona switches the cellular uptake mechanism and trafficking pathway of lipid nanoparticles

An apolipoprotein-enriched biomolecular corona switches the cellular uptake mechanism and trafficking pathway of lipid nanoparticles

PECAM-1 Leu125Val (rs688) Polymorphism and Diabetic Nephropathy in Caucasians with Type 2 Diabetes Mellitus

Deployment and exploitation of nanotechnology nanomaterials and nanomedicine

Contact Info

Product

Resources

About