Nanotechnologies for society. New designs and applications of nanosensors and nanobiosensors in medicine and environmental analysis

Chomoucká, Jana; Drbohlavová, Jana; Masařík, Michal; Ryvolová, Markéta; Húska, Dalibor; Prášek, Jan; Horna, Aleš; Trnková, Libuše; Provazník, Ivo; Adam, Vojtěch; Hubálek, Jaromír; Kizek, René

doi:10.1504/ijnt.2012.046752

Cited by 19 publications

(6 citation statements)

References 220 publications

(208 reference statements)

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“…The development of biocompatible, highly fluorescent nanoparticles including quantum dots (QDs) for chemical and biochemical labeling, immunoanalysis, molecular imaging, and/or targeted therapy is a field attracting an extensive attention . The main reason is that nanometer‐sized particles have functional and structural properties that are not available from either discrete molecules or bulk materials . When conjugated with biomolecular affinity ligands, such as antibodies, peptides, or small molecules, these nanoparticles can be used to target specific molecules such as DNA, proteins, and/or even cells .…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic study of peptide‐mediated quantum dot‐human immunoglobulin bioconjugation

et al. 2013

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The bioconjugation of quantum dots (QDs) is a key process in their application for bioanalysis as well as imaging. The coupling of QDs with biologically active molecules such as peptides, nucleic acids, and/or antibodies enables their fluorescent labeling, and therefore, selective and sensitive tracking during the bioanalytical process, however, the efficiency of the labeling and preservation of the biological activity of the bioconjugate have to be considered. In this study, a new approach of the bioconjugation of CdTe-QDs and human immunoglobulin employing a small peptide is described. The heptapeptide (HWRGWVC) was synthesized and characterized by mass spectrometry, liquid chromatography, and capillary electrophoresis. Moreover, the peptide was used as a capping agent for QDs synthesis. The CdTe-QDs were synthesized by microwave synthesis (600 W, 20 min) using 3.2 mM CdCl2 and 0.8 mM Na2TeO3. The bioconjugation of QDs capped by this peptide with immunoglobulin was investigated by capillary electrophoresis and magnetic immunoextraction coupled with electrochemical detection by differential pulse voltammetry. Furthermore, the applicability of prepared bioconjugates for fluorescent immunodetection was verified using immobilized goat antihuman IgG antibody.

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Section: Introductionmentioning

confidence: 99%

Electrophoretic study of peptide‐mediated quantum dot‐human immunoglobulin bioconjugation

et al. 2013

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“…[3][4][5] Nanobiosensors, which afford rapid detection at a potentially low cost, usually contain a capture agent on the sensor surface to bind the target biomolecule in a selective and specic manner. [6][7][8] The effectiveness of these biosensing devices depends on a number of factors including the surface immobilization chemistry and micro-/nano-fabrication techniques used. Substrate functionalization plays a crucial role here because the orientation and functionality of bioreceptor molecules have a strong inuence on the overall performance of the biosensor.…”

Section: Introductionmentioning

confidence: 99%

Dendrimeric antigen–silica particle composites: an innovative approach for IgE quantification

Vida¹,

Montañez²,

Collado³

et al. 2013

J. Mater. Chem. B

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“…The published results suggest that the CNT/Cu composite electrode detector displays enhanced sensitivity compared with detectors based on copper or CNTs alone. This result could be related to specific properties of copper on the surfaces of the various types of electrodes . One can conclude that significant improvements in the performance of a CE microchip with an electrochemical detector are observed using a CNT‐modified working electrode.…”

Section: Detection Enhancementmentioning

confidence: 95%

CE and nanomaterials – Part II: Nanomaterials in CE

Adam

Vaculovičová

2017

Electrophoresis

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The scope of this two-part review is to summarize publications dealing with CE and nanomaterials together. This topic can be viewed from two broad perspectives, and this article is trying to highlight these two approaches: (i) CE of nanomaterials, and (ii) nanomaterials in CE. The second part aims at summarization of publications dealing with application of nanomaterials for enhancement of CE performance either in terms of increasing the separation resolution or for improvement of the detection. To increase the resolution, nanomaterials are employed as either surface modification of the capillary wall forming open tubular column or as additives to the separation electrolyte resulting in a pseudostationary phase. Moreover, nanomaterials have proven to be very beneficial for increasing also the sensitivity of detection employed in CE or even they enable the detection (e.g., fluorescent tags of nonfluorescent molecules).

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Nanotechnologies for society. New designs and applications of nanosensors and nanobiosensors in medicine and environmental analysis

Abstract: One of the areas rapidly gaining popularity in all parts of scientific research is Nanotechnology. The pioneering mechanical and electrical engineering has been overtaken by life sciences including chemistry and biology as well as biophysics and biomedicine. The attractiveness of

Cited by 19 publications

References 220 publications

Electrophoretic study of peptide‐mediated quantum dot‐human immunoglobulin bioconjugation

Electrophoretic study of peptide‐mediated quantum dot‐human immunoglobulin bioconjugation

Dendrimeric antigen–silica particle composites: an innovative approach for IgE quantification

CE and nanomaterials – Part II: Nanomaterials in CE

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