Nanotextured titanium surfaces for enhancing skin growth on transcutaneous osseointegrated devices

Puckett, Sabrina; Lee, Phin Peng; Ciombor, Deborah McK.; Aaron, Roy K.; Webster, Thomas J.

doi:10.1016/j.actbio.2009.12.016

Cited by 92 publications

(69 citation statements)

References 61 publications

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“…Several studies reported elongated cell morphology on TNTs of similar dimensions [48] and fibroblast-like shape on nanotextured titania produced by etching and other methods. [51][52][53] These cellular behaviors are considered to be related to the size of the cell-surface contact [54] and to the amount of adsorbed proteins which can be recognized by integrin transmembrane receptors. In this study, the diameter of the nanotubes was ≈70 nm, which was reported to prevent integrin clustering.…”

Section: Cell Morphologymentioning

confidence: 99%

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

Zhukova

Hiepen

Knaus

et al. 2017

Adv Healthcare Materials

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Surface structuring of titanium-based implants is known to modulate the behavior of adherent cells, but the influence of different nanotopographies is poorly understood. The aim is to investigate preosteoblast proliferation, adhesion, morphology, and migration on surfaces with similar surface chemistry but distinct nanotopographical features. Sonochemical treatment and anodic oxidation are employed to fabricate disordered, mesoporous titania (TMS) and ordered titania nanotubular (TNT) topographies on titanium, respectively. Morphological evaluation reveals that cells are polygonal and well-spread on TMS, but display an elongated, fibroblast-like morphology on TNT surfaces, while they are much flatter on glass. Both nanostructured surfaces impair cell adhesion, but TMS is more favorable for cell growth due to its support of cell attachment and spreading in contrast to TNT. A quantitative wound healing assay in combination with live-cell imaging reveals that cell migration on TMS surfaces has a more collective character than on other surfaces, probably due to a closer proximity between neighboring migrating cells on TMS. The results indicate distinctly different cell adhesion and migration on ordered and disordered titania nanotopographies, providing important information that can be used in optimizing titanium-based scaffold design to foster bone tissue growth and repair while allowing for the encapsulation of drugs into porous titania layer

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Section: Cell Morphologymentioning

confidence: 99%

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

Zhukova

Hiepen

Knaus

et al. 2017

Adv Healthcare Materials

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“…16,[20][21][22] This material has served as a carrier for several agents such as proteins, antibacterial compounds, and other drugs. 18,[22][23][24][25] In addition, titania nanotubes are highly ordered with different diameters and lengths that can be easily fabricated to specification.…”

Section: Introductionmentioning

confidence: 99%

“…These methods include altering surgical techniques, modifying the implant design, and coating its surface. 16 Coating approaches have been shown to increase the antibacterial ability of implants by using fibroblast growth factor 2-embedded apatite composites 17 and silver nanoparticles. 18,19 Although these coatings reduced the presence of bacteria compared to their uncoated counterparts, the infection rate was still high and there was no reported effect on biologic seal formation.…”

Section: Introductionmentioning

confidence: 99%

Increased fibroblast functionality on CNN2-loaded titania nanotubes

Wei¹,

Wu²,

Feng³

et al. 2012

IJN

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Infection and epithelial downgrowth are major problems associated with maxillofacial percutaneous implants. These complications are mainly due to the improper closure of the implant-skin interface. Therefore, designing a percutaneous implant that better promotes the formation of a stable soft tissue biologic seal around percutaneous sites is highly desirable. Additionally, the fibroblast has been proven to play an important role in the formation of biologic seals. In this study, titania nanotubes were filled with 11.2 kDa C-terminal CCN2 (connective tissue growth factor) fragment, which could exert full CCN2 activity to increase the biological functionality of fibroblasts. This drug delivery system was fabricated on a titanium implant surface. CCN2 was loaded into anodized titania nanotubes using a simplified lyophilization method and the loading efficiency was approximately 80%. Then, the release kinetics of CCN2 from these nanotubes was investigated. Furthermore, the influence of CCN2-loaded titania nanotubes on fibroblast functionality was examined. The results revealed increased fibroblast adhesion at 0.25, 0.5, 1, 2, 4, and 24 hours, increased fibroblast viability over the course of 5 days, as well as enhanced actin cytoskeleton organization on CCN2-loaded titania nanotubes surfaces compared to uncoated, unmodified counterparts. Therefore, the results from this in vitro study demonstrate that CCN2-loaded titania nanotubes have the ability to increase fibroblast functionality and should be further studied as a method of promoting the formation of a stable soft tissue biologic seal around percutaneous sites.

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“…[5][6][7][8][9][10] Less literature is available on the activities of inflammatory cells on defined, nanostructured surfaces, [11][12][13] even though these cells are among the first to encounter an implanted device and play a decisive role in the acceptance of the implant. It has also been suggested that nanostructured materials play a role in bacterial adhesion.…”

Section: Introductionmentioning

confidence: 99%

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

Svensson¹,

Forsberg²,

Hulander³

et al. 2014

IJN

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The role of material surface properties in the direct interaction with bacteria and the indirect route via host defense cells is not fully understood. Recently, it was suggested that nanostructured implant surfaces possess antimicrobial properties. In the current study, the adhesion and biofilm formation of Staphylococcus epidermidis and human monocyte adhesion and activation were studied separately and in coculture in different in vitro models using smooth gold and well-defined nanostructured gold surfaces. Two polystyrene surfaces were used as controls in the monocyte experiments. Fluorescent viability staining demonstrated a reduction in the viability of S. epidermidis close to the nanostructured gold surface, whereas the smooth gold correlated with more live biofilm. The results were supported by scanning electron microscopy observations, showing higher biofilm tower formations and more mature biofilms on smooth gold compared with nanostructured gold. Unstimulated monocytes on the different substrates demonstrated low activation, reduced gene expression of pro- and anti-inflammatory cytokines, and low cytokine secretion. In contrast, stimulation with opsonized zymosan or opsonized live S. epidermidis for 1 hour significantly increased the production of reactive oxygen species, the gene expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10, as well as the secretion of TNF-α, demonstrating the ability of the cells to elicit a response and actively phagocytose prey. In addition, cells cultured on the smooth gold and the nanostructured gold displayed a different adhesion pattern and a more rapid oxidative burst than those cultured on polystyrene upon stimulation. We conclude that S. epidermidis decreased its viability initially when adhering to nanostructured surfaces compared with smooth gold surfaces, especially in the bacterial cell layers closest to the surface. In contrast, material surface properties neither strongly promoted nor attenuated the activity of monocytes when exposed to zymosan particles or S. epidermidis .

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Nanotextured titanium surfaces for enhancing skin growth on transcutaneous osseointegrated devices

Cited by 92 publications

References 61 publications

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

Increased fibroblast functionality on CNN2-loaded titania nanotubes

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

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