Nanotoxicity of Magnesium Oxide on Human Neuroblastoma SH-SY5Y Cell Lines

Abstract

The use of magnesium oxide (MgO) nanoparticles in industrial applications has been raised over the last decade. However, there is limited toxicology information available regarding the effects of MgO nanoparticles. In this study, cytotoxicity and neurotoxicity of this nanoparticle on SH-SY5Y cell lines was investigated. In order to assess the cytotoxicity effect, SH-SY5Y cells were exposed to three different types of MgO nanoparticles (MgO 5, MgO 10 and MgO 24) for 24, 48 and 72 h. The concentration of nanoparticles ranges from 1nM to 1mM. Cell viability was determined by MTS assay. Neurotoxicity test was performed to determine the effects of MgO nanoparticles on human neural cells. Results indicated that MgO nanoparticles are not toxic to both undifferentiated and differentiated SH-SY5Y cells. With further exploration, the safety and health concern regarding exposure of MgO nanoparticles can be verified since the increasing of using this nanoparticle in industry over the time.

Biochemical and molecular evidences on the protection by magnesium oxide nanoparticles of chlorpyrifos-induced apoptosis in human lymphocytes

“…About MgO NPs, in that respect are some articles, which have indicated toxic properties[ 22 34 ] and also other articles, which have exhibited the positive effects of MgO NPs on different organisms by changing the concentration and size of MgO NPs. [ 18 23 35 ] Hence, in this work we prospected increasing maintenance of the human lymphocytes by testing different concentrations of MgO NPs.…”

“…Magnesium oxide nanoparticle (MgO NP) is one of the metal oxide NPs, which is extensively used in several industries such as pharmaceuticals, cosmetics, food packaging, electrical insulation, desiccant, and medicine. [ 18 ] It has the advantage of nontoxic, high thermal stability, biocompatible, low cost, and has considerable potential as an antibacterial agent. [ 19 ] A number of studies have also been performed on MgO NPs and have evaluated different mechanistic endpoints.…”

“…(2013) indicated that MgO NPs have no toxicity on human neuroblastoma SH-SY5Y cell lines. [ 18 ] All these researches mentioned above indicated that the beneficial or detrimental biological effects of MgO NPs were dependent on particle composition, particle concentration, particle size, exposure time, and the nature of the biological target.…”

In vivo Toxicity Investigation of Magnesium Oxide Nanoparticles in Rat for Environmental and Biomedical Applications

“…The applications of MgO nanoparticles and also the issues on its feasible toxicity are increasing (16). Unfortunately, there is a paucity of knowledge about the effect of the prolonged exposure to nanoparticles on human health and the environment.…”

“…Additionally, the cytotoxicity and neurotoxicity of MgO nanoparticles on SH-SY5Y cell line have also been investigated, and the results showed that MgO nanoparticles are not toxic in concentration ranges from 1nM to 1mM for 24, 48, and 72 h to both undifferentiated and differentiated SH-SY5Y cells. MTS method was used for cell viability (16).…”