Nanotoxicity of Rare Earth Metal Oxide Anchored Graphene Nanohybrid: A Facile Synthesis and <i>In Vitro</i> Cellular Response Studies

De, S. S.; Mohanty, Smita; Nayak, Sanjay K.; Verma, Suresh K.; Suar, Mrutyunjay

doi:10.1142/s1793292015500915

Cited by 6 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Graphene is made up of a sp 2 bonded carbon atoms, arranged in a single layer, existing as a 2-D honeycomb structure. It shows properties which are unique such as quantum Hall effect, very high electron mobility, excellent electrical conductivity, large surface to volume ratio so it can absorb a large amount of aromatic biomolecules by π-π stacking [64] , tuneable optical properties, and high mechanical strength, which make it an ideal nanomaterial [65] , [66] for potential application in medical and biological fields as a biosensor [11] . It has been used for the detection of different varieties of biomolecules such as Deoxyribonucleic Acid (DNA), antibodies, enzymes, aptamers, etc.…”

Section: Characteristics and Classification Of Graphene And Its Derivmentioning

confidence: 99%

Graphene based sensors

Shahdeo

Roberts

Abbineni

et al. 2020

Comprehensive Analytical Chemistry

View full text Add to dashboard Cite

Section: Characteristics and Classification Of Graphene And Its Derivmentioning

confidence: 99%

Graphene based sensors

Shahdeo

Roberts

Abbineni

et al. 2020

Comprehensive Analytical Chemistry

View full text Add to dashboard Cite

“…The nanohybrid with CR moiety effectively inhibit free radicals, as proven by DPPH assay, which is thought to be the vital key in projecting superior cancer cytotoxicity effects, through counteracting and redox modulation of the built-in high ROS and inflammatory intracellular tumor environment [125]. In fact, redox-modulation cytotoxicity symbolizes the prominent pathway for both CR-based nano-formulations, and nano-toxicology materials in general as reported in previous research [121, 126–131].…”

Section: Resultsmentioning

confidence: 95%

“…Both cell lines showed distinct size and morphology alteration compared to the untreated control. Cellular shrinkage and proliferation inhibition were observed in CAG treatment groups, which increased with higher CAG concentration, suggesting a dose-dependent trend [121, 122]. Furthermore, morphological changes were studied with starting raw material GO, and traditional citrate-synthesized nanocomposite rGO-AuNPs, as a comparative approach (S2 Fig).…”

Section: Resultsmentioning

confidence: 99%

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

et al. 2019

View full text Add to dashboard Cite

Nanotechnology-based antioxidants and therapeutic agents are believed to be the next generation tools to face the ever-increasing cancer mortality rates. Graphene stands as a preferred nano-therapeutic template, due to the advanced properties and cellular interaction mechanisms. Nevertheless, majority of graphene-based composites suffer from hindered development as efficient cancer therapeutics. Recent nano-toxicology reviews and recommendations emphasize on the preliminary synthetic stages as a crucial element in driving successful applications results. In this study, we present an integrated, green, one-pot hybridization of target-suited raw materials into curcumin-capped gold nanoparticle-conjugated reduced graphene oxide (CAG) nanocomposite, as a prominent anti-oxidant and anti-cancer agent. Distinct from previous studies, the beneficial attributes of curcumin are employed to their fullest extent, such that they perform dual roles of being a natural reducing agent and possessing antioxidant anti-cancer functional moiety. The proposed novel green synthesis approach secured an enhanced structure with dispersed homogenous AuNPs (15.62 ± 4.04 nm) anchored on reduced graphene oxide (rGO) sheets, as evidenced by transmission electron microscopy, surpassing other traditional chemical reductants. On the other hand, safe, non-toxic CAG elevates biological activity and supports biocompatibility. Free radical DPPH inhibition assay revealed CAG antioxidant potential with IC 50 (324.1 ± 1.8%) value reduced by half compared to that of traditional citrate-rGO-AuNP nanocomposite (612.1 ± 10.1%), which confirms the amplified multi-potent antioxidant activity. Human colon cancer cell lines (HT-29 and SW-948) showed concentration- and time-dependent cytotoxicity for CAG, as determined by optical microscopy images and WST-8 assay, with relatively low IC 50 values (~100 μg/ml), while preserving biocompatibility towards normal human colon (CCD-841) and liver cells (WRL-68), with high selectivity indices (≥ 2.0) at all tested time points. Collectively, our results demonstrate effective green synthesis of CAG nanocomposite, free of additional stabilizing agents, and its bioactivity as an antioxidant and selective anti-colon cancer agent.

show abstract

“…A high-profile research is carried out on graphene and its derivatives in biological applications. [24][25][26] In some in vivo and in vitro studies both graphene and GO did not show cytotoxicity to bones. 27 In another in vitro study done on glioma Cells, it is found that GO is less toxic than rGO.…”

Section: Introductionmentioning

confidence: 96%

<p>Synthesis of Graphene Oxide Using Atmospheric Plasma for Prospective Biological Applications</p>

Alam

Park

et al. 2020

IJN

View full text Add to dashboard Cite

Introduction This paper presents a novel technique for the synthesis of graphene oxide (GO) with various surface features using high-density atmospheric plasma deposition. Furthermore, to investigate the use of hydrophobic, super-hydrophobic, and hydrophilic graphene in biological applications, we synthesized hydrophobic, super-hydrophobic, and hydrophilic graphene oxides by additional heat treatment and argon plasma treatment, respectively. In contrast to conventional fabrication procedures, reduced graphene oxide (rGO) formed under low pressure and high-temperature environment using a new synthesis method—developed and described in this study—offers a convenient deposition method on any kind surface with controlled wettability. Methods High density at atmospheric plasma is used for the synthesis of rGO and GO and its biocompatibility based on various wetting properties was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the viability of cells in response to rGO and GO with various surface features was investigated. Structural integrity was characterized by Raman spectroscopy, FESEM and FE-TEM. Wettability was measured via contact angle method and confirmed with XPS analysis. Results We found that GO coating with a hydrophilic feature is more biocompatible than other surfaces as observed in case of fibroblast cells. We have shown that wettability—controlled by GO deposition—influences biocompatibilities and antibacterial effect of biomaterial surfaces. Discussion Measuring the contact angle, it is found that contact angle for hydrophobic is increased to 150.590 and reduced to 11.580 by heat and argon plasma treatment, respectively, from 75.880 that was initially in the case of hydrophobic surface. XPS analysis confirmed various oxygen-containing functional groups transforming as deposited hydrophobic surface into superhydrophobic and hydrophilic surface. Thus, we have proposed a new, direct, cost-effective, and highly productive method for the synthesis of rGO and GO—with various surface properties—for biological applications. Similarly, for the dental implant application, the Streptococcus mutans was used as an antibacterial effect and found that S. mutans grows slowly on hydrophilic surface. Thus, antibacterial effect was prominent on GO with hydrophilic surface.

show abstract

Nanotoxicity of Rare Earth Metal Oxide Anchored Graphene Nanohybrid: A Facile Synthesis and In Vitro Cellular Response Studies

Cited by 6 publications

References 52 publications

Graphene based sensors

Graphene based sensors

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

<p>Synthesis of Graphene Oxide Using Atmospheric Plasma for Prospective Biological Applications</p>

Contact Info

Product

Resources

About