Nanozyme amplification mediated on-demand multiplex lateral flow immunoassay with dual-readout and broadened detection range

Liu, Sijie; Dou, Leina; Yao, Xiaolin; Zhang, Wentao; Zhao, Man; Yin, Xuechi; Sun, Jing; Zhang, Daohong; Wang, Jianlong

doi:10.1016/j.bios.2020.112610

Cited by 95 publications

(53 citation statements)

References 29 publications

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“…After assay optimization, LODs of about 20 and 34 CFU mL −1 were obtained for Salmonella enteritidis and E. coli O157:H7, respectively. A LFIA based on PB nanozyme was also proposed by Liu et al [102]. The authors reported about a magnetic-PB nanozyme which enables a colorimetric simultaneous detection of ractopamine (RAC) and clenbuterol (CLE).…”

Section: Nanozymesmentioning

confidence: 99%

“…Indeed, the LOD obtained by visual approach was 1.0 ng mL −1 both for RAC and CLE, while it decreased to 0.12 and 0.20 ng mL −1 for RAC and CLE, respectively by measuring the colorimetric intensity of the lines. A LFIA based on PB nanozyme was also proposed by Liu et al [102]. The authors reported about a magnetic-PB nanozyme which enables a colorimetric simultaneous detection of ractopamine (RAC) and clenbuterol (CLE).…”

Section: Nanozymesmentioning

confidence: 99%

“…A LFIA based on PB nanozyme was also proposed by Liu et al [ 102 ]. The authors reported about a magnetic-PB nanozyme which enables a colorimetric simultaneous detection of ractopamine (RAC) and clenbuterol (CLE).…”

Section: Nanozymesmentioning

confidence: 99%

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Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Calabria

Calabretta

Zangheri

et al. 2021

Sensors

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Paper-based lateral-flow immunoassays (LFIAs) have achieved considerable commercial success and their impact in diagnostics is continuously growing. LFIA results are often obtained by visualizing by the naked eye color changes in given areas, providing a qualitative information about the presence/absence of the target analyte in the sample. However, this platform has the potential to provide ultrasensitive quantitative analysis for several applications. Indeed, LFIA is based on well-established immunological techniques, which have known in the last year great advances due to the combination of highly sensitive tracers, innovative signal amplification strategies and last-generation instrumental detectors. All these available progresses can be applied also to the LFIA platform by adapting them to a portable and miniaturized format. This possibility opens countless strategies for definitively turning the LFIA technique into an ultrasensitive quantitative method. Among the different proposals for achieving this goal, the use of enzyme-based immunoassay is very well known and widespread for routine analysis and it can represent a valid approach for improving LFIA performances. Several examples have been recently reported in literature exploiting enzymes properties and features for obtaining significative advances in this field. In this review, we aim to provide a critical overview of the recent progresses in highly sensitive LFIA detection technologies, involving the exploitation of enzyme-based amplification strategies. The features and applications of the technologies, along with future developments and challenges, are also discussed.

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Section: Nanozymesmentioning

confidence: 99%

Section: Nanozymesmentioning

confidence: 99%

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Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Calabria

Calabretta

Zangheri

et al. 2021

Sensors

View full text Add to dashboard Cite

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“…The evidence of Fe3O4 nanozyme for enhanced detection Ebola virus with 100 times enhancement compared to the conventional AuNPs-based LFI, revealing the signal amplification ability of nanozyme [120]. Various fascinating nanozyme, such as AuPt nanoflowers [121], Pt nanocatalyst [122], Pt-Ni(OH)2 nanosheets [123], Prussian blue NPs (PBNPs) [124], have been used to construct LFI, and realized their widely application in food safety. For For colorimetric LFI, AuNPs are the common signal labeled material for visual output through non-covalent electrostatic adsorption of antibodies or antigens [113].…”

Section: Nanozyme For Signal Amplificationmentioning

confidence: 99%

Advances in Visual Immunoassays for Sensitive Detection of Mycotoxins in Food—A Review

Liang

2021

The 1st International Electronic Conference on Chemical Sensors and Analytical Chemistry

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Mycotoxins are the toxic secondary metabolites naturally produced by fungi; their contamination in agricultural products and food severely threatens food safety and public health worldwide. The reliable, efficient, and sensitive quantification of mycotoxins in food has become increasingly challenging to tackle due to the complexity of food matrices and their low level. Visual detection has emerged as a popular trend toward miniaturization and simplification of mycotoxins assays yet is constrained with their limited sensitivity. This review mainly focuses on the various sensitive visual immunoassays for signal amplified detection of mycotoxins. These signal amplified immunoassays for the improved sensitivity of mycotoxins detection in food through nanomaterials for encapsulation enzyme, enzyme-mediated nanomaterials as the amplified signal readout, and nanozyme. Furthermore, the underlying principle and the advantages of visual immunoassays for mycotoxins have been proposed. And the challenges and perspectives have been proposed to develop improved efficient visual immunoassays for mycotoxins in food.

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“…Third, simultaneous detection of SARS-CoV-2/FluA in the ICA platform can be easily realized by setting two test (T) lines on the strip, thereby improving the detection efficiency. However, traditional ICA method has two inherent defects including limited sensitivity and poor quantitative ability due to the use of colloidal gold (AuNP)-based colorimetric analysis [ 25 , 26 ]. These defects hinder the large-scale implementation of ICA for respiratory virus diagnosis.…”

Section: Introductionmentioning

confidence: 99%