Naoxintong inhibits myocardial infarction injury by VEGF/eNOS signaling-mediated neovascularization

Wang, Hong; Qiu, Li-Zhen; Ma, Ya-ke; Zhang, Lusha; Chen, Lu; Li, Chunxiao; Geng, Xiao; You, Xingyu; Gao, Xiumei

doi:10.1016/j.jep.2017.06.040

Cited by 25 publications

(16 citation statements)

References 47 publications

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“…NO was synthesized from the amino acid L-arginine by either the constitutive endothelial NOS (eNOS) or the inducible NOS (iNOS) [26]. It has been reported in previous studies that VEGF can increase the expression of eNOS and secretion of NO [7,8,27,28]. In the current study, we observed that 630 mg/kg and 1260 mg/kg YC significantly increased in the expression of eNOS in testes of the CPtreated mice in vivo, and 0.1 and 1 mg/mL YCE also significantly increased the secretion of NO and the expressions of eNOS in MLTC-1 cells in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…It was regarded as important candidates for the regulation of physiological and pathophysiological angiogenesis [6]. e VEGF was also an endothelium-specific secreted protein that potently stimulated vasodilation and microvascular hyperpermeability [7,8]. Among the angiogenic factors, VEGF is a multifunctional protein, and it played a key role in new blood vessel formation.…”

Section: Introductionmentioning

confidence: 99%

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Yangjing Capsule Can Improve the Function of the Testicular Angiogenesis through Activating VEGFA/eNOS Signaling Pathway

Jin

Sun

Dong

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. The testicular microcirculation was an important aspect of testicular physiology and it offered a stable environment for the transport of nutrients and secretary products in the testis. Yangjing capsule (YC), a traditional Chinese compound herbal prescription, has been proved as an effective drug to ameliorate spermatogenesis, promote testosterone synthesis in vivo, and cure spermatogenesis in clinical practice. Objective. This study was aimed at understanding the potential mechanisms of YC exerting angiogenic effects in the mouse spermatogenesis dysfunction model induced by cyclophosphamide (CP) and MLTC-1 cells. Materials and Methods. Balb/c mice were randomly divided into five groups: control, CP, CP plus YC (630 mg/kg), CP plus YC (1260 mg/kg), and CP plus YC (2520 mg/kg). After 30 days, mice were sacrificed and the expressions of endothelial marker CD34+, angiogenic marker VEGFA, VEGFR1, VEGFR2, and eNOS in the testes of the mice were examined; moreover, Leydig cell line MLTC-1 cells were cultured and treated with different concentrations of YC extracts (YCE), and the expressions of VEGFA, VEGFR1, VEGFR2, and eNOS, as well as the secretion of NO, were evaluated. Results. We observed that YC significantly increased the expressions of VEGFA, VEGFR1, VEGFR2, and eNOS in testes of CP-treated mice; moreover, YCE has led to increased expressions of VEGFA, VEGFR1, VEGFR2, and eNOS and secretion of NO in MLTC-1 in vitro. These data suggested that the YC might be an alternative treatment for the dysfunction of testicular microcirculation by promoting the angiogenesis in the testis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Yangjing Capsule Can Improve the Function of the Testicular Angiogenesis through Activating VEGFA/eNOS Signaling Pathway

Jin

Sun

Dong

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…herbs Olibanum (Ruxiang) and Myrrha (Moyao), and 3 animal herbs Pheretima (Dilong), Scorpio (Quanxie) and Hirudo (Shuizhi) [1]. NXTC is also one of commercial medicinal products approved by the China Food and Drug Administration and listed in the Chinese Pharmacopoeia 2015 with the effect of invigorating qi and activating blood circulation, removing blood stasis and dredging collaterals [2].…”

Section: Introductionmentioning

confidence: 99%

“…Promoting blood circulation and removing blood stasis is the basic pharmacological action of NXTC in the treatment of cardiovascular and cerebrovascular diseases [5]. The active chemical ingredients of NXTC mainly include avonoids, avonoid glycosides, phenanthraquinones, phenolic acids, terpenoids, and polysaccharide, which are related to the pharmacological functions of NXTC on inhibition of platelet aggregation, anti-in ammation, reduction of apoptosis and ROS production, activation of lipid metabolism, promotion of angiogenesis and lesion plaque stability, attenuation of vascular calci cation [1,[6][7][8][9]. Many researchers have been trying to explore and identify the major and bioactive constituents of NXTC, and many chemical constituents in NXTC in vitro were quanti ed [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics comparison of four major bio-active components in normal and blood stasis rats after administration of Naoxintong Capsule by UPLC-TQ/MS

Li¹,

Chen²,

Zhang³

et al. 2020

Preprint

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Background Blood stasis is one major cause of cardiovascular and cerebrovascular diseases. Naoxintong capsule (NXTC), a Chinese patent medicine, has been widely employed in the prevention and treatment against cardiovascular and cerebrovascular diseases. However, the pharmacokinetics comparison of NXTC in normal and blood stasis rats were remain obscured. Methods Acute blood stasis rats were induced by being placed in ice-cold water during the interval between two injections of adrenaline hydrochloride. Normal and blood stasis rats were administrated of NXTC suspension at the dosage of 5 g⋅kg -1 , and blood was collected at different time points after then. Concentrations of four main components including caffeic acid, ferulic acid, formononetin and tanshinone IIA in rat plasma were quantified by UPLC-TQ/MS. The pharmacokinetic parameters were calculated by Phoenix WinNonlin v6.2 software. Results It was found that C max , AUC all , AUC INF_obs , Vz_F_obs and MRT last of ferulic acid, AUC all, Vz_F_obs and MRT last of caffeic acid in blood stasis rats were significantly different ( P < 0.05) from normal rats. Compared with normal rats, C max of ferulic acid and formononetin decreased significantly in the plasma of acute blood stasis rats, AUC all of caffeic acid and ferulic acid decreased notably, AUC INF_obs of ferulic acid decreased remarkably, Vz_F_obs and MRT last of ferulic acid and caffeic acid increased reversely. It is suggested that the absorption of the four components of NXTC in acute blood stasis rats was weakened, and the elimination time was prolonged. Conclusions The significant difference in some different parameters of the 4 NXTC components in normal and blood stasis rats might be caused by increasing of blood viscosity and slowing down of blood flow in acute blood stasis rats. The pharmacokinetic study under pathological condition provided important information for more rational use of NXTC in clinical situations.

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“…For several thousand years, preparations of earthworms (dilong) in raw form have been widely used in traditional medicine in China for the treatment of vascular disorders . In Chinese medicine, it is given in the form of decoctions such as Bu Yang Huan Wu and Naoxintong in treating ischemic injury . Fibrinolytic enzymes from earthworms are generally very stable and are effective in treating thrombosis associated with myocardial and cerebral infarction.…”

Section: Introductionmentioning

confidence: 99%

Pheretima aspergillum extract attenuates high‐KCl‐induced mitochondrial injury and pro‐fibrotic events in cardiomyoblast cells

Huang

Shibu

Kuo

et al. 2019

Environmental Toxicology

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Naoxintong inhibits myocardial infarction injury by VEGF/eNOS signaling-mediated neovascularization

Cited by 25 publications

References 47 publications

Yangjing Capsule Can Improve the Function of the Testicular Angiogenesis through Activating VEGFA/eNOS Signaling Pathway

Yangjing Capsule Can Improve the Function of the Testicular Angiogenesis through Activating VEGFA/eNOS Signaling Pathway

Pharmacokinetics comparison of four major bio-active components in normal and blood stasis rats after administration of Naoxintong Capsule by UPLC-TQ/MS

Pheretima aspergillum extract attenuates high‐KCl‐induced mitochondrial injury and pro‐fibrotic events in cardiomyoblast cells

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