NAPE-PLD regulates specific baseline affective behaviors but is dispensable for inflammatory hyperalgesia

Chen, I‐Jen; Murdaugh, L. S.; Miliano, Cristina; Dong, Yan; Gregus, Ann M.; Buczynski, Matthew W.

doi:10.1016/j.ynpai.2023.100135

Cited by 8 publications

(5 citation statements)

References 83 publications

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“…OFT was performed 2 h after completion of vapor exposure as we have described previously 22 . The mouse’s position was assessed by AnyMaze software using an overhead camera to measure the total distance traveled (m), time spent immobile (s), time spent in edge or center zone, and number of center entries.…”

Section: Methodsmentioning

confidence: 99%

“…LDT was performed at 2 and 24 h after completion of vapor exposure as we have described previously 22 . Time spent on either side (s), latency to exit from the dark chamber (s), and average dark visits (s) were recorded.…”

Section: Methodsmentioning

confidence: 99%

“…The Splash test protocol was conducted as we described previously 22 2 h after the last vapor exposure using tap water. The total time spent grooming was recorded.…”

Section: Methodsmentioning

confidence: 99%

“…The sucrose preference test was performed as we described previously 22 by exposing the mice to two bottles with either tap water or 1% sucrose, in a 2-h session at 2 and 24 h after cessation of vapor exposure. Each bottle was weighed before and after testing, and sucrose preference was calculated as the % sucrose consumed relative to the total liquid intake ((sucrose consumed (g)/total liquid consumed (g)) × 100) for each session.…”

Section: Methodsmentioning

confidence: 99%

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Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice

Murdaugh,

Miliano,

Chen

et al. 2024

Sci Rep

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Nicotine use is a leading cause of preventable deaths worldwide, and most of those who attempt to quit will relapse. While electronic cigarettes and other electronic nicotine delivery systems (ENDS) were presented as a safer alternative to traditional cigarettes and promoted as devices to help traditional tobacco smokers reduce or quit smoking, they have instead contributed to increasing nicotine use among youths. Despite this, ENDS also represent a useful tool to create novel preclinical animal models of nicotine exposure that more accurately represent human nicotine use. In this study, we validated a chronic, intermittent, ENDS-based passive vapor exposure model in mice, and then measured changes in multiple behaviors related to nicotine abstinence. First, we performed a behavioral dose curve to investigate the effects of different nicotine inter-vape intervals on various measures including body weight, locomotor activity, and pain hypersensitivity. Next, we performed a pharmacokinetic study to measure plasma levels of nicotine and cotinine following chronic exposure for each inter-vape interval. Finally, we utilized a behavior test battery at a single dosing regimen that produces blood levels equivalent to human smokers in order to characterize the effects of chronic nicotine, vehicle, or passive airflow and identified nicotine-induced impairments in cognitive behavior.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The Splash test protocol was conducted as we described previously 22 2 h after the last vapor exposure using tap water. The total time spent grooming was recorded.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice

Murdaugh,

Miliano,

Chen

et al. 2024

Sci Rep

Self Cite

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“…Morris Water Maze 19,20 , Barnes Maze 16 ) and recognition tasks (e.g. novel object recognition [21][22][23] ) have been implemented to measure cognitive function in large cohorts of mice 12,[24][25][26][27][28] , including chemically-induced rodent pain models such as Chemotherapy Induced Peripheral Neuropathy [29][30][31] and Complete Freund's Adjuvant 32 which exhibit a limited period of pain sensitivity. However, these paradigms rely on hippocampalbased spatial memory and the HPA-driven stress-influenced motivated behavior that provide an experimental challenge for evaluating executive function and other subdomains of cognitive behavior.…”

Section: Introductionmentioning

confidence: 99%

Examining Cognitive Performance in Mice using the Open-Source Operant Feeding Device FED3

Murdaugh,

Brown,

Chen

et al. 2024

Preprint

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Cognitive impairments are prevalent in various neurological disorders, including chronic pain conditions, and pose significant therapeutic challenges. Preclinical rodent models serve as valuable tools for investigating the underlying mechanisms of and treatments for cognitive dysfunction. However, factors such as stress, age, sex, and disease duration present challenges to reliably capturing cognitive deficits in rodents. Here, we present a comprehensive and high-throughput protocol utilizing the open-source operant Feeding Experimentation Device 3 (FED3) for assessing cognitive performance in mice. We developed a data pipeline to streamline data compilation and analysis, and established operating conditions for a six-test cognitive battery which can be completed in as few as 20 days. We validated our testing procedures using bilateral orbitofrontal cortical lesions to capture deficits in executive function, and demonstrated the feasibility of assessing cognitive function in aged mice of both sexes to identify genotypic and sex-specific effects. Overall, our findings demonstrate that the FED3 is a versatile tool for evaluating cognitive function in mice, offering a low-cost, high-throughput approach for preclinical studies of neurological disorders. We anticipate that this protocol will facilitate broader implementation of cognitive testing in rodent models and contribute to the understanding and treatment of cognitive dysfunction in neurological diseases.

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12/15-Lipoxygenases mediate neuropathic-like pain hypersensitivity in female mice

Brown,

Chen,

Miliano

et al. 2024

Preprint

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It is estimated that chronic neuropathic pain conditions exhibit up to 10% prevalence in the general population, with increased incidence in females. However, nonsteroidal inflammatory drugs (NSAIDs) are ineffective, and currently indicated prescription treatments such as opioids, anticonvulsants, and antidepressants provide only limited therapeutic benefit. In the current work, we extended previous studies in male rats utilizing a paradigm of central Toll-like receptor 4 (TLR4)-dependent, NSAID-unresponsive neuropathic-like pain hypersensitivity to male and female C57BL/6N mice, uncovering an unexpected hyperalgesic phenotype in female mice following intrathecal (IT) LPS. In contrast to previous reports in female C57BL/6J mice, female C57BL/6N mice displayed tactile and cold allodynia, grip force deficits, and locomotor hyperactivity in response to IT LPS. Congruent with our previous observations in male rats, systemic inhibition of 12/15-Lipoxygenases (12/15-LOX) in female B6N mice with selective inhibitors - ML355 (targeting 12-LOX-p) and ML351 (targeting 15-LOX-1) - completely reversed allodynia and grip force deficits. We demonstrate here that 12/15-LOX enzymes also are expressed in mouse spinal cord and that 12/15-LOX metabolites produce tactile allodynia when administered spinally (IT) or peripherally (intraplantar in the paw, IPLT) in a hyperalgesic priming model, similar to others observations with the cyclooxygenase (COX) metabolite Prostaglandin E2 (PGE2). Surprisingly, we did not detect hyperalgesic priming following IT administration of LPS, indicating that this phenomenon likely requires peripheral activation of nociceptors. Collectively, these data suggest that 12/15-LOX enzymes contribute to neuropathic-like pain hypersensitivity in rodents, with potential translatability as druggable targets across sexes and species using multiple reflexive and non-reflexive outcome measures.

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NAPE-PLD regulates specific baseline affective behaviors but is dispensable for inflammatory hyperalgesia

Cited by 8 publications

References 83 publications

Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice

Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice

Examining Cognitive Performance in Mice using the Open-Source Operant Feeding Device FED3

12/15-Lipoxygenases mediate neuropathic-like pain hypersensitivity in female mice

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