Narciclasine: an Antimitotic Substance from Narcissus Bulbs

Ceriotti, G

doi:10.1038/213595a0

Cited by 185 publications

(132 citation statements)

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“…The A maryllidaceae alkaloids studied here have a potent antitumor activity [ 14,15]. The results presented in this paper show that haemanthamine, lycorine, pretazettine and pseudolycorine inhibit protein synthesis in yeast cell-free systems by blocking the peptide bond formation step, as previously reported with the chemically and biologically related substance narciclasine [9].…”

Section: Discussionsupporting

confidence: 65%

Yeast ribosomal sensitivity and resistance to the Amaryllidaceae alkaloids

1975

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Section: Discussionsupporting

confidence: 65%

Yeast ribosomal sensitivity and resistance to the Amaryllidaceae alkaloids

1975

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“…It demonstrates a wide range of inhibitory effects on plant growth and development. The mode of action of NCS on the susceptible plant cells is not known although it has been shown to inhibit protein synthesis (Carrasco et al 1975) and exhibit antimitotic (Ceriotti 1967) and antiviral activity (Gabrielsen et al 1992). Our previous study (Bi et al 1998) indicated that NCS inhibited the growth of excised radish cotyledons.…”

Section: Discussionmentioning

confidence: 99%

Narciclasine alters chloroplast membrane structure and inhibits 5‐aminolevulinic acid and chlorophyll binding protein accumulation in wheat (Triticum aestivum) leaves

Zhang

Guo

et al. 2003

New Zealand Journal of Crop and Horticultural Science

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Narciclasine (NCS), an Amaryllidaceae alkaloid from Narcissus tazetta bulbs, is a potent plant growth inhibitor. It shows a broad range of inhibitory effects on seed germination and seedling growth in wheat (Triticum aestivum). In this study, the chlorophyll content of light-grown wheat seedlings was markedly reduced in the presence of NCS. In etiolated wheat leaf sections, the greening process initiated upon illumination was inhibited by NCS. The block of chlorophyll accumulation in the presence of NCS was most probably due to the block of the formation of 5-aminolevulinic acid, an essential chlorophyll precursor. Western blot analysis showed that the formation of light harvesting chlorophyll a/b binding protein (LHCII) was also inhibited by NCS. Electron microscopy studies provided direct observation that the formation of chloroplast and internal membrane † Corresponding author. systems were blocked when etioplasts were illuminated in the presence of NCS. Thus, our study showed that NCS inhibited chlorophyll and chloroplast protein accumulation, concomitantly chloroplast formation.

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“…It has been shown that narciclasine exhibits a wide range of inhibitory effects on plant growth including seed germination, seedling growth, growth and plastid development of excised radish cotyledons (inhibiting the transition of glyoxysomes and peroxisomes), and chloroplast development (9). The mode of action of narciclasine is not known, although it has been shown to inhibit protein synthesis (10), isocitrate lyase, and hydroxypyruvate reductase activities in glyoxysomes and peroxisomes (9), respectively, and to exhibit antimitotic (11) and antiviral (12) activities. McLachlan et al (13) showed that pancratistatin, the chemical structure of which is very close to that of narciclasine, induces rapid apoptosis in SHSY-5Y neuroblastoma cells but at pharmacologic doses.…”

Section: Introductionmentioning

confidence: 99%

Narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma cells

Lefranc

Sauvage

Goietsenoven

et al. 2009

Molecular Cancer Therapeutics

107

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Cell motility and resistance to apoptosis characterize glioblastoma multiforme growth and malignancy. Narciclasine, a plant growth modulator, could represent a powerful new weapon targeting the Achilles' heel of glioblastoma multiforme and may offer the potential to better combat these devastating malignancies. The in vitro effects of narciclasine on cell proliferation, morphology, actin cytoskeleton organization, and the Rho/Rho kinase/LIM kinase/cofilin pathway and its antitumor activity in vivo have been determined in models of human glioblastoma multiforme. Narciclasine impairs glioblastoma multiforme growth by markedly decreasing mitotic rates without inducing apoptosis. The compound also modulates the Rho/Rho kinase/LIM kinase/cofilin signaling pathway, greatly increasing GTPase RhoA activity as well as inducing actin stress fiber formation in a RhoA-dependent manner. Lastly, the treatment of human glioblastoma multiforme orthotopic xenograft-bearing mice with nontoxic doses of narciclasine significantly increased their survival.Narciclasine antitumor effects were of the same magnitude as those of temozolomide, the drug associated with the highest therapeutic benefits in treating glioblastoma multiforme patients. Our results show for the first time that narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma multiforme cells and significantly increases the survival of human glioblastoma multiforme preclinical models. This statement is made despite the recognition that to date, irrespective of treatment, no single glioblastoma multiforme patient has been cured.

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Narciclasine: an Antimitotic Substance from Narcissus Bulbs

Cited by 185 publications

References 1 publication

Yeast ribosomal sensitivity and resistance to the Amaryllidaceae alkaloids

Yeast ribosomal sensitivity and resistance to the Amaryllidaceae alkaloids

Narciclasine alters chloroplast membrane structure and inhibits 5‐aminolevulinic acid and chlorophyll binding protein accumulation in wheat (Triticum aestivum) leaves

Narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma cells

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