Naringin protects myocardial cells from doxorubicin-induced apoptosis partially by inhibiting the p38MAPK pathway

Abstract

Doxorubicin (DOX) has been widely used to treat cancers as a first-line antitumor drug. However, it causes severe, irreversible, dose-dependent cardiotoxicity. To evaluate the protective effects of naringin (NRG) on cardiotoxicity, the authors investigated the molecular mechanism of the p38MAPK signaling pathway. H9c2 cells were treated for 24 h by using 5 µmol/l DOX without or with being pretreated by 1 µM NRG for 150 min or by 3 µM SB203580 for 60 min. Cell viability was detected by cell counting kit-8 assay. Intracellular reactive oxygen species (ROS) levels were detected based on the oxidative conversion of 2′,7′-dichlorfluorescein-diacetate (cell-permeable) to dichlorofluorescein (fluorescent). The expression of p38MAPK was determined by western blotting. The expression level of p-p38MAPK in H9c2 cells, which was significantly increased by exposure to 5 µM DOX for 60 min (P<0.01), was significantly decreased by pretreatment with 1 µM NRG for 150 min beforehand (P<0.01). The viability of H9c2 cells pretreated for 150 min with 1 µM NRG was significantly enhanced compared with that using DOX directly (P<0.01). Intracellular ROS levels were significantly reduced by being pretreated with 1 µM NRG for 150 min or with 3 µM SB203580 for 60 min before the cells were exposed to 5 µM DOX. Collectively, NRG protected H9c2 cells against the cardiotoxicity induced by DOX through suppressing the expression and activity of the p38MAPK pathway. The findings provided valuable evidence for the possible use of NRG to relieve DOX-induced cardiotoxicity.

Protective effects of S‐carvedilol on doxorubicin‐induced damages to human umbilical vein endothelial cells and rats

“…ROS levels have been reported to be involved in DOX‐induced cell apoptosis (Jian et al, ; Namazi, Sepehri, Delphi, & Moridi, ). Superoxide and peroxynitrite are the major ROS generated by DOX (Mihm, Yu, Weinstein, Reiser, & Auer, ; Luanpitpong et al, ).…”

Astragaloside IV ameliorates endoplasmic reticulum stress‑induced apoptosis of Aβ25‑35‑treated PC12 cells by inhibiting the p38�MAPK signaling pathway

“…Jian et al reported that treatment with SB203580, a p38 MAPK inhibitor, inhibited ROS generation in myocardial cells, thus protecting the cells from doxorubicin-induced apoptosis (25). The results of the present study also demonstrated that treatment with 20 mM Aβ 25-35 induced p38 MAPK activation, consistent with recent studies (26,27).…”

Ameliorative effect of rosiglitazone, a peroxisome proliferator gamma agonist on adriamycin‐induced cardio toxicity via suppressing oxidative stress and apoptosis

“…It was further dried out, implanted on squares of paraffin, and cut into the pieces of around 4–6 mm thickness pursued by hematoxylin and eosin (H&E) staining. At that point, the samples were seen under the microscope by histopathologist …”