Naringin Supplementation during Pregnancy Induces Sex and Region-Specific Alterations in the Offspring’s Brain Redox Status

Abstract

Research has shown the beneficial effects of naringin supplementation to adult rodents, which can ameliorate oxidative stress in disease models. However, evidence has demonstrated that polyphenol supplementation induced detrimental effects when consumed during sensitive periods of development, such as pregnancy. Therefore, we investigated the effect of maternal naringin supplementation during pregnancy on the offspring’s cerebral redox status. Pregnant Wistar rats were divided into control and naringin groups and supplemented from gestational day 15 to gestational day 21. On postnatal days 1, 7, and 21, offspring were euthanized, and the prefrontal cortex, hippocampus, striatum, and cerebellum dissected. On postnatal day 1, maternal naringin supplementation positively modulated the pups’ brain redox status. On postnatal day 7, a pro-oxidative milieu was observed in the offspring’s striatum and cerebellum in a sex-dependent manner, even though the prefrontal cortex and hippocampus were not negatively affected. Besides, the alterations observed on postnatal day 7 did not persist up to weaning. Our findings demonstrated that the effect induced by naringin supplementation in the brain redox status differed according to the period of development in which naringin was consumed since the beneficial effects usually found in the adult rodents became detrimental when the supplementation was applied during pregnancy.

Late pregnancy maternal naringin supplementation affects the mitochondria in the cerebellum of Wistar rat offspring via sirtuin 3 and AKT

“…Naringin's ability to influence mitochondrial health highlights its potential to be used as a therapy in pregnancy‐related diseases and prevent offspring neuronal damage due to mitochondrial dysfunction and oxidative stress. In fact, recent findings have shown that both naringin and naringenin consumption during pregnancy may affect the offspring's brain redox status during postnatal life and induce energetic metabolism by modulating the electron transport system (ETS) and tricarboxylic acid (TCA) cycle enzymes in the pups' cerebellum early in life (August et al, 2020, 2021; August & dos Santos, 2020; Gindri dos Santos et al, 2021). However, the mechanisms by which naringin and naringenin affect offspring's mitochondria and whether such alterations might induce neurodevelopmental and behavioral defects that might prevent its use during pregnancy are still largely unknown.…”

Effect of naringin on sodium fluoride‑induced neurobehavioral deficits in Wistar rats

“…Naringin is isolated from citrus family plants such as lemons and oranges. Naringin is a 4',5,7-trihydroxyflavanone-7-rhamnoglucoside that tastes bitter and has shown beneficial effects in conditions such as diabetes, hepatic diseases and neurological diseases (7)(8)(9)(10)(11). It possesses antioxidant and anti-inflammatory properties, along with hypolipidemic activities (12).…”