Narrowbore high-performance liquid chromatography for the simultaneous determination of sildenafil and its metabolite UK-103,320 in human plasma using column switching

Jeong, Chang Kyun; Lee, Hee-Yong; Jang, Min-Sun; Kim, Won; Lee, Hye Suk

doi:10.1016/s0378-4347(00)00536-3

Cited by 32 publications

(15 citation statements)

References 10 publications

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“…Several ml of biological fluids can be directly injected on this extraction material without loss of performances [115,116]. Capcell Pak MF [115][116][117][118][119][120][121][122][123][124][125][126][127][128][129][130][131][132][133] and Capcell Pak SCX [134] materials were suitable tools for the direct determination of drug-containing biofluids.…”

Section: Mixed-functional Materialmentioning

confidence: 99%

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

Souverain

Rudaz

Veuthey

2004

Journal of Chromatography B

250

124

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Section: Mixed-functional Materialmentioning

confidence: 99%

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

Souverain

Rudaz

Veuthey

2004

Journal of Chromatography B

250

124

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“…Phosphate buffer (70:30). Jeong 2001 (Jeong, et al, 2001) detected SC and its metabolite in human plasma using column switching HPLC method. Daraghmeh 2001(Daraghmeh, et al, 2001 estimated the SC in commercial and market formulation using ACN and Ammonium acetate as mobile phase.…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC Method Development for Estimation of Sildenafil Citrate in Tablets and in Seminal Fluid

Sharma¹

2012

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A RP HPLC method for estimation of sildenafil citrate (SC) in tablet dosage form and seminal fluid was developed and validated. Best resolution was obtained with column Waters Spherisorb® C 18 bonded silica, (5 µm, 4.6 x 250 mm) at 230 nm with retention time of 5.01 min. The mobile phase used was TEA (0.2%) pH adjusted at 3 with OPA and ACN (60:40) with flow rate of 1.0 mL/min. The method for estimation of sildenafil citrate in tablet dosage form was found to be linear, accurate, precise, sensitive and selective. Whereas the bioanalytical estimation of SC in seminal fluid was found to be in the range of 100 ng/mL to 10µg/mL. Method was found to be highly sensitive as LOD and LOQ were found to 0.3 µg/ml and 0.9µg/ml. The repeatability and reproducibility were within the range i.e. less than 2%.The accuracy of the method was 99.3%. The percentage purity was calculated for market formulation was 102.8%. The internal standard used for bioanalytical methods was Diclofenac sodium. The drug was extracted from seminal fluid by protein precipitation using ACN as precipitating agent. The linearity range was from 100.0ng/mL to 2.0µg/mL. The LOD and LOQ were found to 0.03µg/mL and 0.1µg/mL. The repeatability and reproducibility were within the range i.e. % RSD less than 15%. The accuracy of the method was 90.36%.and the extraction efficiency was found to be 98.25%. The stability of drug was found to be within the range i.e. less the 15%. INTRODUCTIONSildenafil citrate marketed as Viagra TM (Pfizer) was approved as a drug for treating male erectile dysfunction (ED) by the US Food and Drug Administration on 27 March 1998 (Anonymous, 1998). SC is 1- [[3-(6,7-dihydro-1-methyl-7-oxo-3-propyl-1H pyrazolo[4,3-d] pyrimidin-5-yl) -4-ethoxyphenyl] sulfonyl]-4-methyl piperazine citrate is a PDE5 inhibitor used for treatment of ED (Anonymous, 2010). Nitric Oxide (NO) is released with sexual stimulation from nerve endings and endothelial cells in the spongy erectile tissue, the corpus cavernosum of the penis. This release of NO activates the enzyme guanylate cyclase. The enzyme guanylate cyclase then converts guanosine triphosphate (GTP) into cGMP causing the smooth muscle to relax, which causes an inflow of blood, which then leads to an erection. Cyclic guanosine monophosphate (cGMP) is then hydrolysed back to the inactive GMP by phosphodiesterase type 5 (PDE5). The level of cGMP is therefore controlled by the activation of cyclic nucleotide cyclase and the breakdown by PDE5.

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“…In column switching, the choice of washing solvent and columnswitching time is crucial in order to obtain complete recovery and clean chromatograms [14]. It was therefore, tested various combinations of the mobile phase composition and pH using acetonitrile and 30 mM sodium phosphate in order to decide the optimum column-switching time.…”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

Sensitive column-switching high-performance liquid chromatography method for determination of propiverine in human plasma

Ban¹,

Maeng²,

Woo³

et al. 2006

Journal of Chromatography B

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Narrowbore high-performance liquid chromatography for the simultaneous determination of sildenafil and its metabolite UK-103,320 in human plasma using column switching

Cited by 32 publications

References 10 publications

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

RP-HPLC Method Development for Estimation of Sildenafil Citrate in Tablets and in Seminal Fluid

Sensitive column-switching high-performance liquid chromatography method for determination of propiverine in human plasma

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