Nasal ectomesenchymal stem cells: Multi-lineage differentiation and transformation effects on fibrin gels

Zhang, Zhijian; He, Q.; Deng, Wenwen; Chen, Qian; Hu, Xinyuan; Gong, Aihua; Cao, Xia; Yu, Jiangnan; Xu, Ximing

doi:10.1016/j.biomaterials.2015.01.057

Cited by 40 publications

(51 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here we report an all‐in‐one, multifunctional niche built solely by a living cell material, ectomesenchymal stem cells (EMSCs), for directed differentiation of NSCs. EMSCs are a peculiar stem cell population originating from the cranial neural crest (NC), a transient structure during embryonic development . EMSCs could be isolated from several adult tissues, such as dental pulp and the olfactory mucosa .…”

mentioning

confidence: 99%

EMSCs Build an All‐in‐One Niche via Cell–Cell Lipid Raft Assembly for Promoted Neuronal but Suppressed Astroglial Differentiation of Neural Stem Cells

Deng

Shao

et al. 2019

Advanced Materials

Self Cite

View full text Add to dashboard Cite

The therapeutic efficiency of allogenic/intrinsic neural stem cells (NSCs) after spinal cord injury is severely compromised because the hostile niche at the lesion site incurs massive astroglial but not neuronal differentiation of NSCs. Although many attempts are made to reconstruct a permissive niche for nerve regeneration, solely using a living cell material to build an all‐in‐one, multifunctional, permissive niche for promoting neuronal while inhibiting astroglial differentiation of NSCs is not reported. Here, ectomesenchymal stem cells (EMSCs) are reported to serve as a living, smart material that creates a permissive, all‐in‐one niche which provides neurotrophic factors, extracellular matrix molecules, cell–cell contact, and favorable substrate stiffness for directing NSC differentiation. Interestingly, in this all‐in‐one niche, a corresponding all‐in‐one signal‐sensing platform is assembled through recruiting various niche signaling molecules into lipid rafts for promoting neuronal differentiation of NSCs, and meanwhile, inhibiting astrocyte overproliferation through the connexin43/YAP/14‐3‐3θ pathway. In vivo studies confirm that EMSCs can promote intrinsic NSC neuronal differentiation and domesticating astrocyte behaviors for nerve regeneration. Collectively, this study represents an all‐in‐one niche created by a single‐cell material—EMSCs for directing NSC differentiation.

show abstract

mentioning

confidence: 99%

EMSCs Build an All‐in‐One Niche via Cell–Cell Lipid Raft Assembly for Promoted Neuronal but Suppressed Astroglial Differentiation of Neural Stem Cells

Deng

Shao

et al. 2019

Advanced Materials

Self Cite

View full text Add to dashboard Cite

show abstract

“…5d). Based on these results above, this upregulated expression of PBMSCs in gel and FP may be attributed to the ideal microenvironment for osteogenic differentiation provided by the three-dimensional space 22 . FP showed the highest protein expression levels.…”

Section: Osteogenic Differentiation Of Pbmscs In Fp Scaffoldsmentioning

confidence: 69%

“…Bioactive properties of biopolymers (collagen, hydrogels, chitosan, and fibrin, etc. ), such as high hydrophilicity and biocompatibility, are conducive to cell proliferation and migration 18,21,22 . Moreover, most of these biopolymers can be induced to form gels under certain conditions.…”

Section: Introductionmentioning

confidence: 99%

“…The two materials were processed by specific methods and integrated to from a fibrin gel/PLGA microsphere (FP) scaffold. Fibrin easily self-assembles to form a gel 22 , and the fibrin gel possesses a structure similar to the extracellular matrix in vivo. The PLGA microspheres were prepared under a double emulsion-evaporation procedure and then stacked into a scaffold with high porosity and interconnected pores distributed within the scaffold.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pre-vascularization in fibrin Gel/PLGA microsphere scaffolds designed for bone regeneration

Chen

et al. 2018

NPG Asia Mater

View full text Add to dashboard Cite

Blood supply plays a central role in alveolar bone regeneration within a large bone defect filled with a cell-laden scaffold material, as it provides sufficient oxygen and nutrition to cells inside the scaffold. To address the issue of insufficient vascularization within scaffolds designed to promote bone regeneration, we developed a pre-vascularized scaffold to enable the repair of large alveolar bone defects. Peripheral blood-derived mesenchymal stem cells (PBMSCs) and endothelial colony-forming cells (ECFCs) were collected from peripheral blood and incorporated into fibrin gel, which was then mixed with poly(lactic-co-glycolic acid) (PLGA) microspheres to form a fibrin gel/PLGA microsphere (FP) scaffold. The induction of osteogenic differentiation of PBMSCs and the pre-vascularization in the FP scaffold were achieved separately under different conditions. PBMSCs seeded into the FP scaffolds with fibrin gel tended to migrate to the surface of PLGA microspheres and express high levels of osteogenic markers. ECFCs cocultured with PBMSCs in FP scaffolds were inclined to form a capillary-like structure in the gel substrate. These capillary-like structures penetrated the space among the microspheres and are supposed to anastomosis with blood capillaries in vivo. Together these results indicate that the pre-vascularized FP scaffold may overcome the shortages of oxygen and nutrition inside conventional scaffolds, leading to a better clinical effect.

show abstract

“…The ability to manipulate cellular microenvironments becomes critically important in miniaturized 3D cell cultures, which include the specific arrangement of cell types, composition of extracellular matrices (ECMs), gradients of soluble factors (growth and differentiation factors), and cell encapsulation materials (natural hydrogels and synthetic polymers) [ 43 , 46 ]. In particular, additives in hydrogels such as ECMs, including elastin, collagen, fibronectin, proteoglycans, laminin and hyaluronic acid can influence cell viability, proliferation and differentiation by controlling signaling pathways in the cells [ 47 ]. The ECM-cell interactions are required to better mimic 3D microenvironments [ 35 ].…”

Section: Factors To Be Considered When Selecting Hydrogels For Micmentioning

confidence: 99%

Biocompatible Hydrogels for Microarray Cell Printing and Encapsulation

2015

View full text Add to dashboard Cite

Conventional drug screening processes are a time-consuming and expensive endeavor, but highly rewarding when they are successful. To identify promising lead compounds, millions of compounds are traditionally screened against therapeutic targets on human cells grown on the surface of 96-wells. These two-dimensional (2D) cell monolayers are physiologically irrelevant, thus, often providing false-positive or false-negative results, when compared to cells grown in three-dimensional (3D) structures such as hydrogel droplets. However, 3D cell culture systems are not easily amenable to high-throughput screening (HTS), thus inherently low throughput, and requiring relatively large volume for cell-based assays. In addition, it is difficult to control cellular microenvironments and hard to obtain reliable cell images due to focus position and transparency issues. To overcome these problems, miniaturized 3D cell cultures in hydrogels were developed via cell printing techniques where cell spots in hydrogels can be arrayed on the surface of glass slides or plastic chips by microarray spotters and cultured in growth media to form cells encapsulated 3D droplets for various cell-based assays. These approaches can dramatically reduce assay volume, provide accurate control over cellular microenvironments, and allow us to obtain clear 3D cell images for high-content imaging (HCI). In this review, several hydrogels that are compatible to microarray printing robots are discussed for miniaturized 3D cell cultures.

show abstract

Nasal ectomesenchymal stem cells: Multi-lineage differentiation and transformation effects on fibrin gels

Cited by 40 publications

References 40 publications

EMSCs Build an All‐in‐One Niche via Cell–Cell Lipid Raft Assembly for Promoted Neuronal but Suppressed Astroglial Differentiation of Neural Stem Cells

EMSCs Build an All‐in‐One Niche via Cell–Cell Lipid Raft Assembly for Promoted Neuronal but Suppressed Astroglial Differentiation of Neural Stem Cells

Pre-vascularization in fibrin Gel/PLGA microsphere scaffolds designed for bone regeneration

Biocompatible Hydrogels for Microarray Cell Printing and Encapsulation

Contact Info

Product

Resources

About